Relation of Age, Race, and Allotype to Immunoglobulin Subclass Concentrations

Shackelford, Penelope G.; Granoff, Dan M.; Nahm, Moon H.; Scott, Mitchell G.; Suarez, Brian K.; Pandey, Janardan P.; Nelson, Susan J.

doi:10.1203/00006450-198508000-00014

Cited by 45 publications

(25 citation statements)

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“…G2m(23) is an allotype on IgG2 molecules found predominantly in Caucasians (2 1). The frequency of this allotype in Caucasians is approximately 57-76% (17,22). In previous studies, Caucasian adults who were negative for G2m(23) had lower serum IgG2 concentrations than those who were G2m(23) positive (3,23).…”

Section: Resultsmentioning

confidence: 93%

Clinical and Immunologic Characteristics of Healthy Children with Subnormal Serum Concentrations of IgG2

et al. 1990

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ABSTRACT. To understand the relevance of subnormal serum concentrations of IgG2, we measured IgG2 in serum of 575 healthy children and identified 11 with concentrations >2 SD less than the mean for age. The levels of IgG2 present were similar to those found in symptomatic children with IgG2 subclass deficiency associated with antibody deficiency. The 11 children ranged in age from 1 to 14 y (mean = 5.7). Detailed clinical information was available on 10 of the 11 children and each was matched for age with two controls. The median number of visitsly to the doctor for infectious illnesses was identical for the two groups (1.0). Nine of the children with subnormal IgG2 were followed for 1 to 5 y (mean = 2.3). All nine children had normal serum concentrations of IgA, IgG1, IgG3, and IgG4 but seven had persistently subnormal or low-normal serum IgG2 concentrations. One of these seven children also had a subnormal serum concentration of IgG, and one had subnormal IgM. Antibody responses to Haemophilus b polysaccharide vaccine were normal in five of six who were immunized. In vitro secretion of Ig by mitogen-stimulated peripheral blood mononuclear cells was measured in six of seven children with persistently subnormal or lownormal IgG2; five showed decreased secretion of IgG2, and two of the five also had subnormal secretion of IgGl and IgG3. An important implication of this study is that the subnormal concentrations of serum IgG2 found in infectionprone children are not a sufficient explanation for their increased susceptibility to infection. The healthy children with low serum concentrations of IgG2 differ from symptomatic children with subnormal IgG2 in that the former have otherwise normal serum Ig concentrations and have normal antibody responses to Hib P S vaccine.

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Section: Resultsmentioning

confidence: 93%

Clinical and Immunologic Characteristics of Healthy Children with Subnormal Serum Concentrations of IgG2

et al. 1990

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“…many studies have reported IgG subclass normal ranges in children and adults [4][5][6][7][8][9][10][11][12][13][14]. and Beard et al [15] have made an interesting comparison of Ihc age-related mean and lower normal limits of IgG subclasses of six studies [6.7,9-!…”

Section: Discussionmentioning

confidence: 99%

“…Sex difference was noted by Beard ef al. [15] in the 50th percentile values of IgG2 and lgG4, Shackelford et al [9] in IgG I and Plebani et al [13] in lgG4. DiiTerence.s in relative proportions of males and females could account for some of the interstudy variations.…”

Section: Discussionmentioning

confidence: 99%

“…The measurement of serum immunoglobulin G subclasses and its interpretation in paediatric age groups has been complicated by both the dilliculty in standardization of" methodologies and reagents [1][2][3] as well as fluctuations in concentrations of IgG subclasses with age [4][5][6][7][8][9][10][11][12][13][14]. The problem of standardization has been thoroughly reviewed and meaningful comparisons between laboratories are still not possible [1.3].…”

Section: Introductionmentioning

confidence: 99%

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Percentile ranges for serum IgG subclass concentrations in healthy Chinese children

Lau

Jones

et al. 1993

Clinical and Experimental Immunology

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SUMMARY[n order lo establish normal reference ranges of serum immunoglobulin G subclasses for different age groups in Chinese, we measured the four IgG subclasses by radial immunodiffusion using poiyclonal antisera in 350 normal healthy subjects (148 males and 202 females) recruited Irom ihe community. There was no signifieant sex difference for all the four IgG subclasses. Using Box-Cox transformation, we constructed smooth age-dependent percentile curves lor the four IgG subclasses. For the 50th percenliie values, the plateaus Tor igGl lo lgG4 are respectively 970 nig/dl at 13 years old. 481 mg/dl al 18. 48 mg/dl at 17 and 80 mg/dl at 13. Methodology, reagents, environmental and genetic factors might be responsible for the observed difference among the various reports. The IgG2 level in our Chinese population seemed to be higher than that in Caucasians, which might account for the very low incidence of invasive Haemophilus influenzae type b disease among the Chinese. Our IgGl level also plateaued later and at a higher level than that in other studies.

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“…If these results are confirmed in larger sample sizes, a number of intriguing possibilities would be raised: could the changes noted be due to immunogenetic differences between CF patients and normals? Immunoglobulin isotype levels are affected not only by antigenic exposure and age, but also gender, race, and genetic differences detectable with immunoglobulin allotypic markers (44). Could the changes observed reflect suboptimal responses to other IgG2-restricted polysaccharide antigens, indicating a subtle underlying humoral immune deficiency?…”

Section: Discussionmentioning

confidence: 99%

Altered Antibody Isotype in Cystic Fibrosis. Possible Role in Opsonic Deficiency

et al. 1986

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ABSTRACT'. Patients with cystic fibrosis (CF) whose respiratory tracts are colonized with Pseudomonas aeruginosa (PA) may develop a specific opsonic deficiency for alveolar macrophage phagocytosis of PA. We examined the possible role of altered antibody (Ab) isotype in this phenomenom by measuring serum levels and distribution of IgG and IgG subclass Ab (IgGl, IgG2, IgG3, and IgG4) to the major opsonic immunodeterminant, serotype-specific lipopolysaccharide (LPS), by means of enzyme-linked immunosorbent assays employing monoclonal secondary antibodies, and comparing these results to the serum opsonic capacity in an in vitro murine alveolar macrophage phagocytic assay. Twenty-one patients with CF who were colonized with PA had approximately a 30-fold elevation of PA LPS IgG Ab levels and higher IgG subclass 1-4 Ab compared to 10 uncolonized patients with CF and 11 healthy controls (p < 0.05-0.0005 depending on the isotype). Colonized patients with CF had a shift in PA LPS Ab distribution toward IgG3 compared to uncolonized patients with CF (p < 0.02). A surprising finding was that uncolonized patients with CF had lower levels (p < 0.05) and proportion (p < 0.002) of PA LPS IgG2 Ab than controls, with an apparent shift to higher levels and proportion of PA LPS IgG4 (p < 0.01). Serum from colonized patients with CF showed diminished opsonic capacity for phagocytosis of PA compared to uncolonized patients and controls (p < 0.005), with 42% showing inhibitory activity.

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Relation of Age, Race, and Allotype to Immunoglobulin Subclass Concentrations

Cited by 45 publications

References 4 publications

Clinical and Immunologic Characteristics of Healthy Children with Subnormal Serum Concentrations of IgG2

Clinical and Immunologic Characteristics of Healthy Children with Subnormal Serum Concentrations of IgG2

Percentile ranges for serum IgG subclass concentrations in healthy Chinese children

Altered Antibody Isotype in Cystic Fibrosis. Possible Role in Opsonic Deficiency

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