Relation of ATPase activities to iron uptake in rabbit and cat erythroid cells

Wise, W. C.; Archdeacon, J. W.

doi:10.1007/bf02144162

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“…Crane postulated that a mobile carrier was capable only of equilibrium. T h e asymmetry required to achieve uphill accumulation of the substance was attributed to a gradient of N a + concentration downhill into the T h e findings in the present experiments and those reported from this l a b o r a t o r y earlier (5,15,16) constitute supporting evidence that the N a -K A T P a s e influences only a small p a r t of iron transport, a n d that the N a -K A T P a s e appears to be i m p o r t a n t in the m o v e m e n t of iron from the cell m e mb r a n e (stroma) to the cell interior.…”

Section: Resultssupporting

confidence: 66%

“…The effect of ouabain in suppressing part of the iron movement from the stroma to the supernate, but with little if any effect on total uptake, implies that it is the movement from the stroma to the supernate and not the movement from the media to the stroma which is N a -K ATPase-dependent. T h e reticulocytes present in the cell suspension are known to take up the 59Fe, since rabbit erythrocytes do not take up a significant amount of iron under experimental conditions where the iron is bound to serum proteins as in these experiments (15,16). J a n d l et al have investigated the possibility that iron uptake in the red cell might be suppressed by inhibition of heme synthesis (2).…”

Section: Resultsmentioning

confidence: 99%

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Accumulation of Iron in the Rabbit Erythroid Cell As Affected by Ouabain, Sodium and Potassium Ions, and Temperature

Wise

Archdeacon

1969

The Journal of General Physiology

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AB STRA C T Reticulocytosis was induced in rabbits with phenylhydrazine. The accumulation of a small part of 59Fe in blood cells of these animals was inhibited by ouabain and related to changes in extracellular sodium and potassium concentrations. Sodium increases movement from the cell surface into the cell, whereas potassium and ouabain decrease this movement. 59Fe movement was found to be temperature-dependent. Thus, the Na-K ATPase system appears to be important in the movement of iron from the cell membrane (stroma) to the cell interior, but influences only a small part of the total iron transport. I N T R O D U C T I O NMovement of iron into the developing red cell is a complex process characterized by (a) formation of an iron-transferrin complex in the plasma, (b) attachment of this complex to a specialized cell surface receptor, (c) release of iron to form a new iron-protein complex, possibly ferritin, and finally (d) the utilization of iron in hemoglobin or other iron compounds in the cell.Katz and J a n d l have reviewed the role of transferrin in the transport of iron to the developing red cell and indicate that iron is delivered by an iron-transferrin complex that becomes attached to the cell surface (1). The metal is actively removed and incorporated in the cell and apparently the transferrin is then displaced by other iron-bearing molecules. In earlier work J a n d l and others showed that iron uptake by the reticulocyte is blocked by several metabolic inhibitors and low oxygen tension (2). Later J a n d l and Katz concluded that, like the uptake of iron, the attachment of transferrin to the surface of the reticulocyte is blocked by such agents as cyanide and 2 , 4 -D N P (3). Morgan has reported studies on interaction between rabbit, human, and rat transferrin and reticulocytes and observed that uptake of transferrin by cells takes place in two steps: first, the formation of a weak union probably by

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%