The possibility of seasonal influences upon salivary calcium levels has been mentioned in a preliminary report of Brawley and Sedwick (4). Seasonal effects on dental caries activity also have been suspected by several authors (11,8, 9,10,12,13). Since the heat regulating mechanism of the body and other general physiologic conditions are greatly affected by climatic variations, such as those of humidity, temperature, air pressre, etc., and food intake; it is quite conceivable that not only dental caries activity but also salivary composition might be affected.The results of a large series of salivary analyses accumulated over a 3 year period, which showed definite relations between the age of subjects, rate of flow, total calcium and inorganic phosphorus content, were evaluated for possible seasonal variations. Because of the large number of individuals examined it was felt that seasonal effects could be noted although different individuals were examined in each season.Age: The results of 650 salivary analyses previously reported (2, 1, 14) were sorted according to the date of analysis and the values obtained over the 3 year period combined by months. Since it was found in previous studies (1, 14) that age influences calcium and phosphorus composition this factor had to be taken into consideration in comparing the values from month to month. The monthly average ages (Table I) grouped together and separated as to sex are presented graphically in figs. lA, B and C. The highest average age (56 years) occurred in the month of August and the lowest (18 years) in October and otherwise they vary considerably.Rate of Flow: The averages of rate of flow (Table I and figs. iD, E and F) show a singular lack of variation by months and the standard deviations are often quite large. In Table II are given the month of analysis, age, and sex of the eleven individuals (2) who because of their low rates of flow (below 0.5 cc./hr.) are not included in the figures or tables and their samples were not sufficient for analysis.Calcium and Phosphorus mg.% and mg./hr.: The calcium mg.% levels shown in figs. 2A, B and C are remarkably constant throughout the year. An exception 1