Relation of Mucoid Growth of
            <i>Staphylococcus aureus</i>
            to Clumping Factor Reaction, Morphology in Serum-Soft Agar, and Virulence

Yoshida, Kenichi; Ekstedt, Richard D.

doi:10.1128/jb.96.4.902-908.1968

Cited by 73 publications

(47 citation statements)

References 5 publications

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“…Medium SM 110A was originally formulated to enhance capsule or slime layer production (Yoshida & Ekstedt 1968). It was therefore worth investigating whether there was a correlation between capsule production and low cell surface hydrophobicity.…”

Section: A P S U L E P R O D U C T I O N a N D Decreased H Y D R O supporting

confidence: 94%

“…All runs were carried out at 18°C in 0.02 mol/l sodium phosphate buffer, pH 64, giving a current voltage of 800 V. a, Strain 1319; b, strain 3407; c, strain Smith diffuse; d, strain Smith compact. (Yoshida & Ekstedt 1968;Caputy & Costerton 1984;Rozgonyi & Seltmann 1985). Our findings, however, suggest that auto-aggregating mastitis and other strains expressing high hydrophobicity maintain very high cell surface hydrophobicity even under conditions which favour production of a hydrophilic capsule or slime layer.…”

Section: Discussionmentioning

confidence: 99%

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Cell surface hydrophobicity and charge of Staphylococcus aureus and coagulase‐negative staphylococci from bovine mastitis

Mamo

Rozgonyi

Brown

et al. 1987

Journal of Applied Bacteriology

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The effects of seven growth media on cell surface hydrophobicity of a collection of Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis were compared in the salt-aggregation test. Thirty-three per cent of Staph. aureus strains showed extremely high cell surface hydrophobicity (auto-aggregated) and 28% were moderately hydrophobic while 26% were hydrophilic after growth on horse blood agar at 37 degrees C for 18 h. There were great variations in the proportion and degree of the hydrophobicity depending on the medium used. Cultivations on/in capsule-inducing media caused a shift from a high to a low degree of hydrophobicity, although a microscopically detectable capsule or slime layer was seen in only one strain. This strain and encapsulated reference strains had a hydrophilic cell surface and migrated faster in free zone electrophoresis than cells of unencapsulated strains. Cells of strains grown on staphylococcus medium 110 agar migrated faster than those grown on horse blood agar regardless of their capsule production. Coagulase-negative staphylococci showed uniformly hydrophilic cell surface after cultivation on horse blood agar, but not when grown in tryptic soy broth or proteose peptone broth. It was concluded that most of the Staph. aureus strains from bovine mastitis under a variety of growth conditions in stationary phase culture constantly expressed hydrophobic cell surface.

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Section: A P S U L E P R O D U C T I O N a N D Decreased H Y D R O supporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Cell surface hydrophobicity and charge of Staphylococcus aureus and coagulase‐negative staphylococci from bovine mastitis

Mamo

Rozgonyi

Brown

et al. 1987

Journal of Applied Bacteriology

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“…Staphylococcal slime is water-soluble and can be removed from the bacterial surface by washing with water [60,61]. Growth on media solidified with agar, preferably under liquid, was at first recommended for the production of extracellular staphylococcal slime, perhaps because the slimy appearance of the bacteria is then very obvious [62,63]. Dry conditions appeared to decrease slime elaboration, and harvesting slime from liquid media was complicated by difficulties in handling large volumes of fluid.…”

Section: Studies With Complex Media and The Use Of Agar As A Gelling mentioning

confidence: 99%

The slime of coagulase-negative staphylococci: Biochemistry and relation to adherence

Hussain

Wilcox

White

1993

FEMS Microbiology Letters

128

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In recent years, infections of implanted plastic devices by coagulase‐negative staphylococci have become a major cause of septicaemia in human patients. The causal bacterial species is usually Staphylococcus epidermidis and these organisms grow as a biofilm adherent to a solid surface. Several methods have been introduced to assess the mass of adherent bacteria and the slimy matrix in which they are embedded. Some methods measure total biofilm, others measure the organisms or the slime alone. In vitro, the type of medium, the atmosphere during incubation, and the nature of the solid surface, affect the quantity of biofilm that is formed. In most studies on the chemistry of the slime, the material used was formed on complex media solidified with agar. Contamination by ingredients of the media or by agar may not always have been recognised. Recent work with chemically defined medium (liquid or solidified with silica gel) shows that the slime is a mixture of about 80% (w/w) teichoic acid and 20% protein. Growth as a biofilm may protect the staphylococci from antibiotics. At present, the greatest success in preventing infection has come from improved surgical techniques during the insertion of implants.

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“…Capsule formation was detected by the wet-film method of Duguid (1951) and by a rapid subculture technique (Opdebeeck et al 1987), using modified medium 110 (Yoshida & Ekstedt 1968). The initial inoculum was from an overnight culture in heart infusion broth at 37°C.…”

Section: P R O D U C T I O N Of Capsules Mucoid Growth a N D C L U Mmentioning

confidence: 99%

Differentiation of Staphylococcus aureus from freshly slaughtered poultry and strains ‘endemic’to processing plants by biochemical and physiological tests

Mead¹,

Norris²,

Bratchell³

1989

Journal of Applied Bacteriology

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A comparison was made of 27 'endemic' strains of Staphylococcus aureus and 35 strains from freshly slaughtered birds, isolated at five commercial slaughterhouses processing chickens or turkeys. Of 112 biochemical and physiological tests used, 74 gave results which differed among the strains. Cluster analysis revealed several distinct groupings which were influenced by strain type, processing plant and bird origin; these included a single group at the 72% level of similarity containing most of the 'endemic' strains. In comparison with strains from freshly slaughtered birds, a higher proportion of 'endemic' strains produced fibrinolysin, alpha-glucosidase and urease and were beta-haemolytic on sheep-blood agar. The 'endemic' type also showed a greater tendency to coagulate human but not bovine plasma, and to produce mucoid growth and clumping. The last two properties, relevant to colonization of processing equipment, were less evident in heart infusion broth than in richer media or process water collected during defeathering of the birds.

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Relation of Mucoid Growth of Staphylococcus aureus to Clumping Factor Reaction, Morphology in Serum-Soft Agar, and Virulence

Cited by 73 publications

References 5 publications

Cell surface hydrophobicity and charge of Staphylococcus aureus and coagulase‐negative staphylococci from bovine mastitis

Cell surface hydrophobicity and charge of Staphylococcus aureus and coagulase‐negative staphylococci from bovine mastitis

The slime of coagulase-negative staphylococci: Biochemistry and relation to adherence

Differentiation of Staphylococcus aureus from freshly slaughtered poultry and strains ‘endemic’to processing plants by biochemical and physiological tests

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