Relations between extraction protocols for activated sludge extracellular polymeric substances (EPS) and EPS complexation properties

Comte, Sophie; Guibaud, Gilles; Baudu, Michel

doi:10.1016/j.enzmictec.2005.06.016

Cited by 382 publications

(234 citation statements)

References 33 publications

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“…2a) display very similar IR spectra, and no noticeable differences appear between the IR spectra of the EPS extracted with physical or control techniques. These similarities between IR spectra from EPS extracted with physical methods are in accordance with results of Comte et al (2006a) obtained with EPS from activated sludges. In contrast, IR spectra of the chemically extracted EPS display different adsorption bands compared to spectra of the control method (Fig.…”

Section: Ir Analysis Of Eps Extractssupporting

confidence: 76%

“…The disruption of the granule by the heating process facilitates their release. Moreover, this method may allow to extract bound EPS (Comte et al 2006a). For this extraction method, the nucleic acid content was determined ( Fig.…”

Section: Organic Composition Of Eps Extracts Of Anaerobic Granular Slmentioning

confidence: 99%

“…Physical or chemical techniques can be used, but there is no standard procedure. Centrifugation is commonly considered as a comparative method because it is the less degradative technique and there is no addition of chemical compounds (Comte et al 2006a). Ultrasonication (Dignac et al 1998), heating (Zhang et al 1999), and cation exchange resins (CER; Frølund et al 1996) are the main physical techniques used.…”

Section: Introductionmentioning

confidence: 99%

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Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols

d’Abzac

Bordas

Hullebusch

et al. 2009

Appl Microbiol Biotechnol

274

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The characteristics of the extracellular polymeric substances (EPS) extracted with nine different extraction protocols from four different types of anaerobic granular sludge were studied. The efficiency of four physical (sonication, heating, cationic exchange resin (CER), and CER associated with sonication) and four chemical (ethylenediaminetetraacetic acid, ethanol, formaldehyde combined with heating, or NaOH) EPS extraction methods was compared to a control extraction protocols (i.e., centrifugation). The nucleic acid content and the protein/ polysaccharide ratio of the EPS extracted show that the extraction does not induce abnormal cellular lysis. Chemical extraction protocols give the highest EPS extraction yields (calculated by the mass ratio between sludges and EPS dry weight (DW)). Infrared analyses as well as an extraction yield over 100% or organic carbon content over 1 gg −1 of DW revealed, nevertheless, a carry-over of the chemical extractants into the EPS extracts. The EPS of the anaerobic granular sludges investigated are predominantly composed of humic-like substances, proteins, and polysaccharides. The EPS content in each biochemical compound varies depending on the sludge type and extraction technique used. Some extraction techniques lead to a slightly preferential extraction of some EPS compounds, e.g., CER gives a higher protein yield.

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Section: Ir Analysis Of Eps Extractssupporting

confidence: 76%

Section: Organic Composition Of Eps Extracts Of Anaerobic Granular Slmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols

d’Abzac

Bordas

Hullebusch

et al. 2009

Appl Microbiol Biotechnol

274

View full text Add to dashboard Cite

show abstract

“…A number of physical and chemical methods have been applied to extract EPS from activated sludge (Sheng et al, 2010). Common physical methods include centrifugation, ultrasonication and heating (Liu and Fang, 2002;Comte et al, 2006;Sheng et al, 2010) while chemical methods include extractions with ethylenediamine tetraacetic acid (EDTA), formaldehyde, NaOH, NaOH-formamide, H 2 SO 4 , and NH 3 (Liu and Fang, 2002;Comte et al, 2006;Adav and Lee, 2008;Sheng et al, 2010). However, the quantities and compositions of EPS extracted by different methods varied in previous studies.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge

Guo

Liu

Xiao

2014

Journal of Biotechnology

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a b s t r a c t Extracellular polymeric substances (EPS) are susceptible to contamination by intracellular substances released during the extraction of EPS owing to the damage caused to microbial cell structures. The damage to cell walls and cell membranes in nine EPS extraction processes of activated sludge was evaluated in this study. The extraction of EPS (including proteins, carbohydrates and DNA) was the highest using the NaOH extraction method and the lowest using formaldehyde extraction. All nine EPS extraction methods in this study resulted in cell wall and membrane damage. The damage to cell walls, evaluated by 2-keto-3-deoxyoctonate (KDO) and N-acetylglucosamine content changes in extracted EPS, was the most significant in the NaOH extraction process. Formaldehyde extraction showed a similar extent of damage to cell walls to those detected in the control method (centrifugation), while those in the formaldehydeNaOH and cation exchange resin extractions were slightly higher than those detected in the control. N-acetylglucosamine was more suitable than KDO for the evaluation of cell wall damage in the EPS extraction of activated sludge. The damage to cell membranes was characterized by two fluorochromes (propidium iodide and FITC Annexin V) with flow cytometry (FCM) measurement. The highest proportion of membrane-damaged cells was detected in NaOH extraction (26.54% of total cells) while membranedamaged cells comprised 8.19% of total cells in the control.

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“…The principal components of activated sludge flocs are bacteria and extracellular polymers substances (EPS). Determination of the chemical composition of EPS has been the objective of many research papers (Frolund et al 1996;Dignac et al 1998;Sponza, 2003), thus proteins and carbohydrates have been identified as the principal components of EPS (Comte et al 2006). Considerably less attention has been devoted to the influence of macromolecular composition of sludge on anaerobic digestion.…”

mentioning

confidence: 99%

Macromolecular composition and anaerobic degradation of the sludge produced in a sequencing batch reactor

Chelme¹,

Fonseca²,

Mercado³

et al. 2008

Electron. J. Biotechnol.

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Relations between extraction protocols for activated sludge extracellular polymeric substances (EPS) and EPS complexation properties

Cited by 382 publications

References 33 publications

Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols

Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols

Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge

Macromolecular composition and anaerobic degradation of the sludge produced in a sequencing batch reactor

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