Relationship among follicular apoptosis, integrin β1 and collagen type IV during early ovarian regression in the teleost Prochilodus argenteus after induced spawning

Santos, Hélio Batista dos; Sato, Yoshimi; Moro, Luciana; Bazzoli, Nilo; Rizzo, Elizete

doi:10.1007/s00441-007-0540-1

Cited by 46 publications

(29 citation statements)

References 59 publications

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“…We therefore presume that 1(IV) is synthesized in and secreted from the theca cells of the follicle to constitute the basement membrane of the follicle layer. Our results are consistent with the recent observations of Santos et al (2008) on the ovary of the curimata-pacu, Prochilodus argenteus. In several mammalian species, collagen IV has also been reported to be immunolocalized to the theca cell compartment for all follicle stages of ovarian follicles (Bortolussi et al 1989;Frojdman et al 1998;Zhao and Luck 1995;Rodgers et al 1998;Irving-Rodgers et al 2002;Iwahashi et al 2000;Berkholtz et al 2006).…”

Section: Discussionsupporting

confidence: 83%

“…The latter include studies showing the presence of collagen type IV in the basement membrane in Xenopus embryos (Bride et al 1982), zebrafish embryos (Crawford and Pilgrim 2005) and adult tissues (MacDonald et al 2009), Drosophila embryos (Medioni and Noselli 2005), and quail embryos (Song et al 2000). However, little is known about the expression and distribution of collagen type IV in the ovaries of non-mammalian species, except for the recent report on the teleost Prochilodus argenteus ovary (Santos et al 2008). That paper dealt with the relationship among follicular apoptosis, integrin β1 and collagen type IV during early ovarian regression after spawning, and documented the localization of immunoreactive materials detectable with anti-human collagen type IV antibody in the fish ovary.…”

Section: Introductionmentioning

confidence: 99%

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Expression and localization of collagen type IV α1 chain in medaka ovary

et al. 2010

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A cDNA clone coding for collagen type IV α1 chain was obtained from the ovary of the medaka, Oryzias latipes. The clone encodes a protein of 1639 amino acids including a putative 21-residue signal peptide, and the deduced amino acid sequence of the α1 chain was homologous to those of the proteins from other species. Collagen type IV α1 chain mRNA was expressed in various tissues of the adult fish. In situ hybridization analysis revealed that the α1 chain mRNA was localized in the follicle layer of all growing follicles.In the post-ovulatory follicle that had released its oocyte in ovulation, the α1 chain transcript was detected in a winding line surrounding the tissue. This localization pattern was different from that of gelatinase B, a marker gene for granulosa cells. A specific antibody was prepared for the medaka collagen type IV α1 chain. Immunohistochemical analysis using this antibody yielded results consistent with those obtained by the in situ hybridization experiment. The current results indicate that in the medaka ovary, collagen type IV is synthesized by theca cells and is localized in the basement membrane.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Expression and localization of collagen type IV α1 chain in medaka ovary

et al. 2010

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“…Immunohistochemical studies in fish have been made using mammalian antibodies, based on evolutionary conservation of proteins (Ortiz-Delgado and Sarasquete 2004;Sharma and Kinsey 2006;Câmara-Pereira et al 2009;Santos et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Distribution of laminin β2, collagen type IV, fibronectin and MMP-9 in ovaries of the teleost fish

Thomé

Santos

Sato³

et al. 2010

J Mol Hist

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Extracellular matrix in the ovarian follicle has been characterised for several mammalian species but there are no reports that describe the immunolocalisation of the extracellular matrix elements, matrix metalloproteinases, and its relation to plasma 17β estradiol levels and follicular apoptosis during the teleost's reproductive cycle. The present study used immunohistochemistry to characterise the distribution of laminin β2, collagen type IV, fibronectin and matrix metalloproteinases-9 (MMP-9). The TUNEL in situ technique was used to quantify apoptosis and indirect immunofluorimetric to determine plasma 17β estradiol levels. The TUNEL-positive reaction associated with morphological features exhibited follicular apoptosis. During postovulatory follicle involution, the drop in plasma 17β estradiol levels after spawning contributed to the intense apoptosis observed. By immunohistochemical analysis, laminin β2 and collagen type IV were identified as the major constituents of the basement membrane. The loss of integrity of the basement membrane occurred due to lyses of the major constituents, and coincides with increased follicular apoptosis. The integrity of the basement membrane is important for the survival of follicular cells. Furthermore, the MMP-9 results suggest that this enzyme is involved in final oocyte maturation and regression of postovulatory follicles. Fibronectin was observed on the surface of follicular cells of the postovulatory follicle in P. argenteus, this being important for maintaining normal cell adhesion to extracellular matrix. In conclusion, our results suggest that the structure and composition of the extracellular matrix, and plasma 17β estradiol levels related to apoptosis, play an important role during the follicular development and post-spawning involution in teleost fishes.

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“…837 When these species are subjected to induced spawning, the final oocyte maturation (resumption of meiosis) occurs, and POFs are formed and quickly reabsorbed in ovaries after spawning. 838 Assessment of autophagy in fish has been primarily made using TEM at different times of ovarian regression. 839 Due to the difficulty of obtaining antibodies specific for each fish species, immunodetection of ATG-proteins (mainly LC3 and BECN1) by immunohistochemistry associated with analyses by western blotting can be performed using antibodies that are commercially available for other vertebrates.…”

Section: 818mentioning

confidence: 99%

Guidelines for the use and interpretation of assays for monitoring autophagy

Klionsky¹,

Abdalla²,

Abeliovich³

et al. 2012

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In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

show abstract

Relationship among follicular apoptosis, integrin β1 and collagen type IV during early ovarian regression in the teleost Prochilodus argenteus after induced spawning

Cited by 46 publications

References 59 publications

Expression and localization of collagen type IV α1 chain in medaka ovary

Expression and localization of collagen type IV α1 chain in medaka ovary

Distribution of laminin β2, collagen type IV, fibronectin and MMP-9 in ovaries of the teleost fish

Guidelines for the use and interpretation of assays for monitoring autophagy

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