Relationship between aberrant methylation of FAS promoter and biological behavior of bladder urothelial carcinoma

Li, Wei; Ding, Xia; Wang, Yuanbao; Li, Yang; Xue, Yanli; Wu, Xin; Ye, Zhangqun

doi:10.1007/s11596-011-0679-6

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…These data further suggest that gene silencing by DNA hypermethylation and histone deacetylation commonly occur on tumor suppressor and proapoptotic genes. Fas downregulation by DNMT1 accumulation has been observed in osteosarcoma and lung carcinoma cell lines (58), and aberrant methylation of Fas promoter was observed in bladder carcinoma (48,59). It has also been reported that DNMT1 silencing upregulates DR5 expression and sensitizes human hepatoma cells to TRAIL-mediated apoptosis (60) and hypermethylation of the DR4 promoter was noted in invasive gastric carcinoma (61).…”

Section: Discussionmentioning

confidence: 95%

Radiation-induced modulation of immunogenic genes in tumor cells is regulated by both histone deacetylases and DNA methyltransferases

Çaçan

Greer

Garnett-Benson

2015

International Journal of Oncology

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Abstract. Radiation treatment is a pivotal therapy for several cancer types, including colorectal cancer. It has been shown that sublethal doses of radiation modulate gene expression, making tumor cells more susceptible to T-cell-mediated immune attack. We have recently shown that low dose radiation enhances expression of multiple death receptors (Fas, DR4 and DR5) and co-stimulatory molecules (4-1BBL and OX-40L) in colorectal cancer (CRC) cells; however, it is unclear how ionizing radiation (IR) enhances expression of these molecules mechanistically. In the present study, we elucidate the molecular mechanisms by which radiation controls expression of these molecules in CRC. Here we report that, enhanced expression of these genes following radiation treatment of CRC cells is due, in part, to changes in DNA methylation and histone acetylation. We observed that radiation (5 Gy) significantly increased histone acetylation at the promoter regions of 4-1BBL, Fas and DR5 but not OX-40L. However, radiation did not induce changes in the global levels of acetylated histone H3 suggesting specificity of IR-induced changes. Furthermore, evaluation of epigenetic controlling enzymes revealed that IR did not alter overall cellular levels of HDACs (HDAC1, HDAC2 or HDAC3) or DNMTs (DNMT1, DNMT3a, or DNMT3b). Instead, radiation decreased binding of HDAC2 and HDAC3 at the promoter regions of Fas and 4-1BBL, respectively. Radiation also resulted in reduced DNMT1 at both the Fas and 4-1BBL promoter regions but not a control gene. We conclude that single dose radiation can influence the expression of immune response relevant genes in colorectal tumor cells by altering the binding of epigenetic enzymes, and modulating histone acetylation, at specific gene promoters.

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Section: Discussionmentioning

confidence: 95%

Radiation-induced modulation of immunogenic genes in tumor cells is regulated by both histone deacetylases and DNA methyltransferases

Çaçan

Greer

Garnett-Benson

2015

International Journal of Oncology

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“…In line our results, Li W et al ., (2011) shown that the rate of FAS promoter methylation in bladder urothelial carcinoma samples is higher than normal samples ( p <0.01) ( 24 ). In addition, a vast literatures have been highlighted the aberrant promoter methylation of FAS gene in different types of cancers including Lymphomas, CXCA, melanoma, Colon, Prostatic and Lung ( 25 - 28 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of methylation and mRNA expression status of FADD and FAS genes in patients with oral squamous cell carcinoma

Dm¹,

Saberi²,

Jamali³

et al. 2014

Med Oral

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Background: Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumorigenesis. Our aims were to analyze the promoter methylation and gene expression profiles of FADD and FAS genes in risk of OSCC. Material and Methods: we analyze the promoter methylation status of FADD and FAS genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also, FADD and FAS genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse-Transcripts PCR. Results: Aberrant promoter methylation of FADD and FAS genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both FADD and FAS genes (P<0.001). The gene expression analysis showed statistically significant difference between cases and healthy controls for both FADD (p<0.02) and FAS (p<0.007) genes. Conclusions: To the best our knowledge, the data of this study are the first report regarding, the effect of promoter hypermethylation of the FADD and FAS genes in development of OSCC. To confirm the data, it is recommended doing further study in large sample sizes in various genetic populations. Key words:OSCC, FADD, FAS, DNA methylation, gene expression.

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“…The scale, prepared by Li (2008), contains five dimensions: altruistic behavior responsibility, respect and care for others, care and care for oneself, altruistic behavior performance, and selfinterested behavior and concepts. There are a total of 22 items in the scale, and the Likert 7-level score from "very inconsistent" to "very consistent" is adopted.…”

Section: Altruistic Behavior Scalementioning

confidence: 99%

Family function and adolescent altruistic behavior: the multiple mediating effects of self-affirmation and psychological resilience

Cui

Zhou

et al. 2023

Front. Psychol.

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IntroductionThe current study aimed to explore the relationship between family function and adolescent altruistic behavior, as well as the mediating effects of self-affirmation and psychological resilience in this relationship.MethodsA survey was conducted on 972 high school students in Guangdong Province using the Family APGAR, GHQSense of Adequacy, Chinese version of Connor-Davidson Resilience Scale, and Altruistic Behavior Scale.ResultsResults found that the score of psychological resilience of males was significantly higher than that of females, but the score of altruistic behavior was significantly lower than that of females. Family function had a positive predictive effect on altruistic behavior. Psychological resilience played a mediating role between family function and altruistic behavior. Self-affirmation and psychological resilience played chain mediating roles between family function and altruistic behavior.DiscussionThis study indicated that family care is crucial for the development of adolescent altruistic behavior, and that it can promote the development of altruistic behavior through the enhancement of self-affirmation and psychological resilience.

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Relationship between aberrant methylation of FAS promoter and biological behavior of bladder urothelial carcinoma

Cited by 7 publications

References 12 publications

Radiation-induced modulation of immunogenic genes in tumor cells is regulated by both histone deacetylases and DNA methyltransferases

Radiation-induced modulation of immunogenic genes in tumor cells is regulated by both histone deacetylases and DNA methyltransferases

Analysis of methylation and mRNA expression status of FADD and FAS genes in patients with oral squamous cell carcinoma

Family function and adolescent altruistic behavior: the multiple mediating effects of self-affirmation and psychological resilience

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