Relationship between antibiotic resistance, biofilm formation, genes coding virulence factors and source of origin of &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; clinical strains

Ratajczak, Magdalena; Kamińska, Dorota; Nowak-Malczewska, Dorota M; Schneider, Anna; Długaszewska, Jolanta

doi:10.26444/aaem/122682

Cited by 24 publications

(20 citation statements)

References 31 publications

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“…In contrast, we defined the antibiotic susceptibility of the different isolates before biofilm production, and associated the susceptibility profile with the biofilm-forming capacity. Similar findings were reported in other bacterial species ( Arciola et al, 2005 ; Klingenberg et al, 2005 ; de Araujo et al, 2006 ; Agarwal and Jain, 2012 ; Yekani et al, 2017 ; Ratajczak et al, 2021 ). For example, Staphylococcus epidermidis strains that produced exopolysaccharide had a significantly higher prevalence of resistance to aminoglycosides, sulfamethoxazole and ciprofloxacin, compared to non-producing isolates.…”

Section: Discussionsupporting

confidence: 88%

Antibiotic Resistance and Biofilm Production Capacity in Clostridioides difficile

Rahmoun

Azrad

Peretz

2021

Front. Cell. Infect. Microbiol.

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BackgroundClostridioides difficile (C. difficile) is one of the primary pathogens responsible for infectious diarrhea. Antibiotic treatment failure, occurring in about 30% of patients, and elevated rates of antibiotic resistance pose a major challenge for therapy. Reinfection often occurs by isolates that produce biofilm, a protective barrier impermeable to antibiotics. We explored the association between antibiotic resistance (in planktonic form) and biofilm-production in 123 C. difficile clinical isolates.ResultsOverall, 66 (53.6%) out of 123 isolates produced a biofilm, with most of them being either a strong (44%) or moderate (34.8%) biofilm producers. When compared to susceptible isolates, a statistically higher percentage of isolates with reduced susceptibility to metronidazole or vancomycin were biofilm producers (p < 0.0001, for both antibiotics). Biofilm production intensity was higher among tolerant isolates; 53.1% of the metronidazole-susceptible isolates were not able to produce biofilms, and only 12.5% were strong biofilm-producers. In contrast, 63% of the isolates with reduced susceptibility had a strong biofilm-production capability, while 22.2% were non-producers. Among the vancomycin-susceptible isolates, 51% were unable to produce biofilms, while all the isolates with reduced vancomycin susceptibility were biofilm-producers. Additionally, strong biofilm production capacity was more common among the isolates with reduced vancomycin susceptibility, compared to susceptible isolates (72.7% vs. 18.8%, respectively). The distribution of biofilm capacity groups was statistically different between different Sequence-types (ST) strains (p =0.001). For example, while most of ST2 (66.7%), ST13 (60%), ST42 (80%) isolates were non-producers, most (75%) ST6 isolates were moderate producers and most of ST104 (57.1%) were strong producers.ConclusionsOur results suggest an association between reduced antibiotic susceptibility and biofilm production capacity. This finding reinforces the importance of antibiotic susceptibility testing, mainly in recurrence infections that may be induced by a strain that is both antibiotic tolerant and biofilm producer. Better adjustment of treatment in such cases may reduce recurrences rates and complications. The link of biofilm production and ST should be further validated; if ST can indicate on isolate virulence, then in the future, when strain typing methods will be more available to laboratories, ST determination may aid in indecision between supportive vs. aggressive treatment.

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Section: Discussionsupporting

confidence: 88%

Antibiotic Resistance and Biofilm Production Capacity in Clostridioides difficile

Rahmoun

Azrad

Peretz

2021

Front. Cell. Infect. Microbiol.

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“… 4 In this study, 54 carbapenem-resistant Pseudomonas aeruginosa strains were isolated from patients in the burn ward. P. aeruginosa poses complex acquired and intrinsic carbapenem resistance mechanisms, 17 , 18 which significantly limits the clinician’s choices for antimicrobial selection and treatments. 19 Therefore, burn patients infected by carbapenem-resistant P. aeruginosa are at higher risk of infection complications.…”

Section: Discussionmentioning

confidence: 99%

Emergence of Carbapenem-Resistant ST244, ST292, and ST2446 Pseudomonas aeruginosa Clones in Burn Patients in Yunnan Province

Fang¹,

Baloch²,

Zhang³

et al. 2022

IDR

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Introduction The prevalence of carbapenem-resistant Pseudomonas aeruginosa is increasing persistently, particularly in burn ward isolates. Here, we investigate the prevalence of carbapenem-resistant Pseudomonas aeruginosa in a burn ward of a provincial-level hospital at Kunming, Yunnan province, China. Methods A total of 118 P. aeruginosa strains were isolated from 57 hospitalized patients, and their MICs were measured. Carbapenem-resistant isolates were selected for multilocus sequence typing (MLST). Carbapenem-resistance mechanisms were identified by examining carbapenemase genes and OprD protein and Carba-NP testing. Representative isolates were further characterized by de novo sequencing for carbapenemase molecular background. Results Among 118 P. aeruginosa isolates, 54 (54/118,45.8%) were carbapenem-resistant Pseudomonas aeruginosa , and 3 genotypes were found (ST292, ST244, and ST2446). Non-carbapenemase-producing ST292 was the most prevalent ST, followed by ST2446 and ST244. A novel 13-bp oprD deletion was found in the ST292 clone, which formed the truncated outer membrane protein and may cause carbapenem resistance. ST244 and ST2446 harbored blaIMP-45 and blaIMP-87 , respectively. blaIMP-45 is located in a megaplasmid, together with aac(6’)-Ib3, blaOXA-1, catB3, qnrVC6, armA, msr(E), mph(E), aph(3’)-Ia, tetC / tetR, aac(6’)-Ib3, floR, mexC-mexD-oprJ, fosA and lead to extensive drug resistance. ST2446 contains a carbapenem-resistant gene blaIMP-87 on the chromosome and is acquired by a novel gene cassette array ( blaIMP-87-ant(2”)-Ia-blaOXA-10-aac(6’)-Ib3 ) of class 1 integron. Discussion For the first time, ST244, ST292 and ST2446 are reported emerging in burn patients, with distinctive carbapenem-resistance mechanisms, respectively. The obtained results highlight the need to surveillance carbapenem-resistant isolates in burn patients.

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“…A study by Elmouaden et al [ 16 ] also reported high proportions of the lasB gene among both MDR strains (99%) and MDS strains (97.6%). In addition, Ratajczak et al [ 17 ] reported the leading contribution of the lasB gene among the majority of the P. aeruginosa strains tested (93.1%). Similar findings were reported in the study conducted by Dawodeyah et al [ 18 ], which revealed that all the MDR strains contained the lasB gene.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, in their study, Sonbol et al [ 38 ] showed a correlation between the occurrence of virulence genes and the resistance mechanisms. Other reports also suggest the high plasticity of the P. aeruginosa genome, providing evidence for the replacement of genes encoding virulence factors with those showing antibiotic resistance or the opposite [ 16 , 17 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Virulence-Factor-Encoding Genes and Genotype Distribution amongst Clinical Pseudomonas aeruginosa Strains

Bogiel

Depka

Kruszewski

et al. 2023

IJMS

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Pseudomonas aeruginosa is an opportunistic pathogen encoding several virulence factors in its genome, which is well-known for its ability to cause severe and life-threatening infections, particularly among cystic fibrosis patients. The organism is also a major cause of nosocomial infections, mainly affecting patients with immune deficiencies and burn wounds, ventilator-assisted patients, and patients affected by other malignancies. The extensively reported emergence of multidrug-resistant (MDR) P. aeruginosa strains poses additional challenges to the management of infections. The aim of this study was to compare the incidence rates of selected virulence-factor-encoding genes and the genotype distribution amongst clinical multidrug-sensitive (MDS) and MDR P. aeruginosa strains. The study involved 74 MDS and 57 MDR P. aeruginosa strains and the following virulence-factor-encoding genes: lasB, plC H, plC N, exoU, nan1, pilA, and pilB. The genotype distribution, with respect to the antimicrobial susceptibility profiles of the strains, was also analyzed. The lasB and plC N genes were present amongst several P. aeruginosa strains, including all the MDR P. aeruginosa, suggesting that their presence might be used as a marker for diagnostic purposes. A wide variety of genotype distributions were observed among the investigated isolates, with the MDS and MDR strains exhibiting, respectively, 18 and 9 distinct profiles. A higher prevalence of genes determining the virulence factors in the MDR strains was observed in this study, but more research is needed on the prevalence and expression levels of these genes in additional MDR strains.

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Relationship between antibiotic resistance, biofilm formation, genes coding virulence factors and source of origin of <i>Pseudomonas aeruginosa</i> clinical strains

Cited by 24 publications

References 31 publications

Antibiotic Resistance and Biofilm Production Capacity in Clostridioides difficile

Antibiotic Resistance and Biofilm Production Capacity in Clostridioides difficile

Emergence of Carbapenem-Resistant ST244, ST292, and ST2446 Pseudomonas aeruginosa Clones in Burn Patients in Yunnan Province

Comparison of Virulence-Factor-Encoding Genes and Genotype Distribution amongst Clinical Pseudomonas aeruginosa Strains

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