Relationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions

Verwilghen, Denis; Busoni, Valéria; Gangl, Monika; Franck, Thierry; Lejeune, Jean-Philippe; Vanderheyden, Laurent; Detilleux, Johann; Grulke, Sigrid; Deberg, Michelle; Henrotin, Yves; Serteyn, Didier

doi:10.1016/j.rvsc.2009.02.002

Cited by 28 publications

(25 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study showed that there was a statistically significant difference (p<0.05) as regard mean S.Coll2-1 levels being higher in group ΙI than group Ι. This was consistent with Henrotin et al (3) who reported that the serum concentrations of Coll2-1 and Coll2-1 NO2 were significantly higher (P<0.05) in knee OA patients with KL III/IV compared to KL I/II patients also; this was in agreement with Denis et al (17). Meanwhile, this was in great discrepancy with Deberg et al (13) who found no relationship between the levels of Coll 2-1 and Coll 2-1 NO2 in the serum and the radiological OA severity.…”

Section: Discussionsupporting

confidence: 90%

Decrease of serum biomarker of type II Collagen degradation (Coll2-1) by intra-articular injection of an autologous plasma-rich-platelet in patients with unilateral primary knee osteoarthritis

2017

View full text Add to dashboard Cite

Objective: This study aimed to evaluate the effect of one dose of intra-articular injection of (PRP) in the knee joint on a specific osteoarthritis (OA) serum biomarker of cartilage degeneration, Collagen 2-1 (Coll2-1), over a short period of 3 months. The aim extended to clarify the effect of PRP on the functional status of the osteoarthritic knee joint. Material and Methods:Sixty patients with primary unilateral knee OA were enrolled in this study. They were subdivided according to Kellgren-Lawrence grading scale (KL) into (Group Ι): including patients with KL grade < 3 and (Group ΙΙ): including patients with KL grade ≥3. Patients were asked to complete the Western Ontario and McMaster Universities Arthritis Index (WOMAC) Score. PRP was prepared and injected immediately into the affected knee. Serum Coll2-1 (S.Coll2-1) concentration was measured by enzyme-linked immunosorbent assay (ELISA) kit pre and 3 months after PRP injection.Results: Significant reduction in S.Coll2-1 concentration in primary knee OA patients; (p<0.001) and (p<0.05) in group Ι and group ΙΙ respectively as well as significant improvements in WOMAC total and WOMAC sub-scores values were noted after single intra-articular PRP injection with maximal functional improvements were achieved after 3 months (p<0.001). Mild cases experienced favorable results with no remarkable adverse reactions were observed. Conclusion:Reduction in specific OA biomarker S.Coll2-1 following intra-articular PRP injection emphasize that PRP could be a promising safe and tolerable effective therapeutic option which improves function from basal states in primary knee OA patients.

show abstract

Section: Discussionsupporting

confidence: 90%

Decrease of serum biomarker of type II Collagen degradation (Coll2-1) by intra-articular injection of an autologous plasma-rich-platelet in patients with unilateral primary knee osteoarthritis

2017

View full text Add to dashboard Cite

show abstract

“…The present study reveals higher levels of cartilage degradation makers in tarsocrural joints affected with osteochondrosis, which underscores the current concept of prophylactic removal of these osteochondral fragments before athletic activity is engaged. In a previous study using serum Coll2-1 in horses, no significant difference was found in horses of a comparable age that were affected with OCD compared to control animals (Verwilghen et al 2009). However, Coll2-1 was only measured in the blood and not directly at the synovial level, which is close to the pathology.…”

Section: Discussionmentioning

confidence: 83%

“…Meaning, this marker can not only differentiate pathologic from non-pathologic joints but also reflect the degree and extent of OA in the affected joint. Yet, plasmatic Coll2-1 concentration was recently introduced as a marker of type-II collagen degradation in horses with osteochondral lesions Verwilghen et al 2009). Nevertheless, measurement of the concentration of this biomarker in synovial fluid of joints affected with OCD has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Coll2-1, Coll2-1NO2 and myeloperoxidase concentrations in the synovial fluid of equine tarsocrural joints affected with osteochondrosis

et al. 2011

Self Cite

View full text Add to dashboard Cite

The measurement of biomarkers that reflect cartilage breakdown is a powerful tool for investigating joint damage caused by disease or injury. Particularly in cases of osteochondrosis, synovial concentrations of these biomarkers may reveal the presence of osteoarthritic changes. Coll2-1, Coll2-1 NO2 and myeloperoxidase have recently been introduced in equine osteoarticular research but comparison between the concentrations of these markers in OCD affected and healthy joints has not been made. Therefore, this study aimed at reporting the synovial concentrations of these biomarkers in joints affected with osteochondral fragments in the tarsocrural joint compared to unaffected joints. Myeloperoxidase and Coll2-1NO2 revealed to have similar levels between affected joints and controls. However, in contrast to previous studies using C2C the present study demonstrated that synovial levels of Coll2-1 were significantly elevated in tarsocrural joints affected with osteochondrosis. Thus, Coll2-1 may be an earlier marker of cartilage degeneration than other cartilage degradation markers that have been previously used in equine medicine.

show abstract

“…Moreover, these findings provide a scientific basis for the traditional uses of Agelanthus dodoneifolius. One of these uses is related to the treatment of asthma, an allergic disease, which is also found in horses (47,48). We can thus expect that this plant could have beneficial effects in the treatment of neutrophils-mediated inflammatory diseases.…”

Section: Discussionmentioning

confidence: 99%

Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity

Boly¹,

Dessy²,

Kohnen³

et al. 2011

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Agelanthus dodoneifolius DC Danser (Loranthaceae)is used for the treatment of various diseases including asthma. The aqueous and hydroalcoholic extracts have been reported to have anti-inflammatory, spasmolytic and bronchorelaxant activities. The present study investigates the effects of the aqueous decoction and the diethyl ether, ethyl acetate and butanolic fractions of Agelanthus dodoneifolius DC Danser (Loranthaceae) on reactive oxygen species (ROS) production and myeloperoxidase (MPO) release by phorbol 12-myristate 13-acetate (PMA)-stimulated equine neutrophils and on purified equine MPO activity. ROS production and MPO release by the PMA-stimulated neutrophils were measured by the lucigenin-enhanced chemiluminescence and ELISA assays, respectively. Specific immunological extraction followed by enzymatic detection (SIEFED) was used to specifically measure the equine MPO activity. Identification and quantification of the individual and total phenolic and flavonoid compounds were performed using UPLC-MS/MS equipment and colorimetric methods involving Folin-Ciocalteu and AlCl 3 , respectively. All the tested extracts displayed dose-dependent inhibitory effects on the oxidant activities of neutrophils; a stronger effect was observed with the organic fractions than the aqueous decoction. These findings could be correlated with a high content of phenolic and flavonoid compounds. The results confirm the previously shown anti-inflammatory effect of Agelanthus dodoneifolius and its potential use for the treatment of neutrophil-dependent inflammatory diseases. IntroductionPolymorphonuclear neutrophils (PMNs) are the primary effector cells in host responses to injury and infection (1-4). The antimicrobial function of stimulated PMNs is based on their phagocytic capacity and their ability to release oxidant and proteolytic enzymes as well as reactive oxygen species (ROS) involved in the destruction of microorganisms. ROS play important roles in the pathogenesis of various serious diseases, such as neurodegenerative disorders, aging, cancer, cataracts, inflammation and cardiovascular diseases including atherosclerosis (5,6).Myeloperoxidase (MPO), a heme peroxidase enzyme stored in the azurophilic granules of neutrophils, plays a crucial role in the destruction of bacteria inside PMNs. The bacterial killing process mainly uses hydrogen peroxide (H 2 O 2 ) and the chloride anion (Cl -) to produce hypochlorous

show abstract

Relationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions

Cited by 28 publications

References 45 publications

Decrease of serum biomarker of type II Collagen degradation (Coll2-1) by intra-articular injection of an autologous plasma-rich-platelet in patients with unilateral primary knee osteoarthritis

Decrease of serum biomarker of type II Collagen degradation (Coll2-1) by intra-articular injection of an autologous plasma-rich-platelet in patients with unilateral primary knee osteoarthritis

Coll2-1, Coll2-1NO2 and myeloperoxidase concentrations in the synovial fluid of equine tarsocrural joints affected with osteochondrosis

Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity

Contact Info

Product

Resources

About