Relationship between clinical phenotype, semen parameters and aneuploidy frequency in sperm nuclei of 50 infertile males

Aneuploidy in human spermatozoa: FISH analysis in men with constitutional chromosomal abnormalities, and in infertile men

“…Studies carried out to investigate the correlation between chromosome aneuploidy and morphologically abnormal spermatozoa by FISH have produced no consistent results (Table 4). No statistical correlations between the frequency of disomy for all the chromosomes analysed and sperm morphology were found in two studies (Rives et al, 1999;Vegetti et al, 2000) of 50 and 19 infertile men, respectively. These results are in agreement with the data obtained by sperm karyotyping using the human spermatozoa-hamster egg fusion technique (Martin and Rademaker, 1988), and after sperm injection into mouse oocytes (Lee et al, 1996).…”

“…Six out of seven studies (86%) did not find any significant association between disomy frequency and sperm motility. Rives et al (1999) reported no correlation between total disomy frequency for the chromosomes analysed and global sperm motility in 50 infertile patients. Aran et al (1999) did not find a significant increase in the frequency of disomy for chromosomes 18, X and Y in five aesthenozoospermic patients.…”

“…Finkelstein et al (1998) found significantly higher frequencies of spermatozoa disomic for all the chromosomes analysed in 12 men with a sperm concentration р 30 ϫ 10 6 ml -1 than in eight donors with a sperm concentration > 60 ϫ 10 6 ml -1 . Rives et al (1999) reported that the mean frequency of disomy for autosomes 1, 13, 14, 18, 21 and 22 (P < 0.001) and gonosomes (P = 0.002) in 50 infertile men increased significantly as sperm concentration decreased. Such a correlation has been confirmed by Vegetti et al (2000) in a study of chromosomes 13, 18, 21, X and Y in 15 infertile men.…”

The Role of Contemporary Andrology in Unraveling the Mystery of Unexplained Male Infertility

“…Moreover, the mean frequency of disomic sperm (presence of two copies of a chromosome) for autosomes and sex chromosomes are 0.13% and 0.37%, respectively, while the rate for diploid sperm (two copies of each chromosome) is 0.2% [39,40]. For normospermic infertile males, the corresponding figures are 0.11%, 0.44% and 0.3-1%, respectively [39,40]. The actual figures of sperm disomy and diploidy for those men with poor semen quality are even higher.…”

Estimation du taux d’aneuploïdies des spermatozoïdes épididymaires prélevés en vue d’ICSI chez des hommes à caryotype normal

“…D'autres 6tudes ont portd sur la recherche de corrdlations entre le taux d'aneuplo'~dies des spermatozo'~des et les anomalies du spermogramme (mobilit6, morphologie, mortali-t6...). Si certains travaux ont conclu ?~ une augmentation du taux d'aneuploidies pour les chromosomes analys6s chez des hommes ~t spermogramme anormal par rapport ~t des tdmoins avec spermogramme normal [1,4,8,11,18,20,22,23,25,26], d'autres n'ont pas retrouv6 de diff6rences [7,12,15]. Ces discordances semblent dues ~t la vafi6t6 des approches m6thodologiques et ~ des interprdtations diff6rentes des valeurs de normalit6 des examens de spermes.…”