Relationship between eosinophil cationic protein and infection intensity in a schistosomiasis endemic community in Ghana

Asuming-Brempong, Elias K.; Gyan, Ben; Amoah, Abena S.; Puije, William van der; Bimi, Langbong; Boakye, Daniel; Ayi, Irène

doi:10.2147/rrtm.s51713

Cited by 2 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experimental data identify eosinophils and their granular proteins as significant participants in the inflammatory process in bronchial asthma [20] and in the interactions between parasite and host [21]. Secretion of the granular contents leads to damage of the bronchial epithelium and late asthmatic reactions, as well as to the killing and disintegration of larvae of various helminths [8,10]. Since the discovery of ECP in 1977, a number of diagnostic kits have been developed to determine its level in biological fluids in various diseases.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Study of eosinophil cationic protein serum levels in patients with toxocariasis

Kaneva¹,

Harizanov²,

Rainova³

et al. 2020

Iberoam J Med

View full text Add to dashboard Cite

Introduction: Toxocariasis is a zoonotic helminth infection with difficult diagnosis. Determination of specific IgG antibodies alone does not allow to establish the disease stage and to evaluate the treatment efficacy. Therefore it is necessary to identify additional markers that will assist the diagnosis. The purpose of our study was to identify and monitor eosinophil cationic protein (ECP) levels in patients with toxocariasis confirmed by serology and to compare our data with the literature to determine the relevance of this protein as an indicator for recent infection and the effectiveness of the therapy. Material and methods: ELISA (CUSABIO) commercial kit was used for determination of ECP concentration. Sixty serum samples were studied from individuals previously tested and confirmed for toxocariasis by the presence of specific anti-Toxocara IgG antibodies in ELISA (Toxocara IgG Rbiopharm) and the presence of specific bands in Western blot as confirmatory test (LD BIO). Twenty serum samples from clinically healthy blood donors were used as a control group. Results: The mean concentration of serum ECP in the patients with toxocariasis was significantly higher than in clinically healthy subjects. Seventy-two percent of patients affected by toxicariasis showed increased serum concentration of ECP. However, no statistically significant differences were observed in terms of age (p = 0.451) and sex (p = 0.682) of the patients or clinical form of the disease. ECP levels among patients with visceral toxocariasis were relatively higher (mean 22.99 ng / ml ± 13.16 SD) in comparison to those with ocular involvement (15.60 ng/ml ± 9.92 SD). Correlation between the presence of peripheral eosinophilia and the concentration of serum ECP was not also established. Conclusion: Data from our study give us reason to believe that serum levels of ECP could serve as an additional marker indicating recent infection, especially in patients without marked increase in the blood eosinophils.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Schistosoma mansoni larvae were incubated with 10 μM (190 μg/ml) ECP and 60% of them died. Concentrations of 50 μM (950 μg/ml) ECP killed 40% of Trypanosoma cruzi in about 6 hours and 90% of Brugia malayi in 48 hours [8][9][10]. Toxocariasis is a parasitic disease caused by the migration of nematode larvae of Toxocara spp.…”

Section: Introductionmentioning

confidence: 99%

Study of eosinophil cationic protein serum levels in patients with toxocariasis

Kaneva¹,

Harizanov²,

Rainova³

et al. 2020

Iberoam J Med

View full text Add to dashboard Cite

show abstract

“…Eosinophil cationic protein (ECP), involved in the body's immune response to foreign pathogens, has also been found to be significantly elevated in the urine of individuals infected with a variety of helminth species including S. haematobium, S. mansoni, O. volvulus , W. bancrofti , and hookworm (Tischendorf et al ., 1999; Tischendorf et al ., 2000; Klion and Nutman, 2004; Fayez et al ., 2010; Asuming-Brempong et al ., 2015). In addition to GDF15, when assessing the use of transrenal ECP as a biomarker for infection with S. haematobium , a positive correlation between expelled ECP and urine egg count was found, suggesting urinal ECP may too increase with infection intensity and may therefore potentially be used to assess disease severity and worm burden (Leutscher et al ., 2008; Leutscher et al ., 2000).…”

Section: Novel Biomarkersmentioning

confidence: 99%

“…In addition to GDF15, when assessing the use of transrenal ECP as a biomarker for infection with S. haematobium , a positive correlation between expelled ECP and urine egg count was found, suggesting urinal ECP may too increase with infection intensity and may therefore potentially be used to assess disease severity and worm burden (Leutscher et al ., 2008; Leutscher et al ., 2000). These findings have since been replicated not only in S. haematobium , but also in S. mansoni infections (Asuming-Brempong et al ., 2015). More recently, ECP levels in serum samples taken from individuals infected with hookworm have also been shown to positively correlate with infection intensity (Amoani et al ., 2019).…”

Section: Novel Biomarkersmentioning

confidence: 99%

An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?

et al. 2019

View full text Add to dashboard Cite

Parasitology cambridge.org/par Review Cite this article: Archer J, LaCourse JE, Webster BL, Stothard JRussell (2020). An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how? Parasitology 147, 873-888. https://doi. AbstractReliable diagnosis of human helminth infection(s) is essential for ongoing disease surveillance and disease elimination. Current WHO-recommended diagnostic assays are unreliable in lowendemic near-elimination settings and typically involve the invasive, onerous and potentially hazardous sampling of bodily fluids such as stool and blood, as well as tissue via biopsy. In contrast, diagnosis by use of non-invasive urine sampling is generally painless, more convenient and low risk. It negates the need for specialist staff, can usually be obtained immediately upon request and is better accepted by patients. In some instances, urine-based diagnostic assays have also been shown to provide a more reliable diagnosis of infection when compared to traditional methods that require alternative and more invasive bodily samples, particularly in low-endemicity settings. Given these relative benefits, we identify and review current research literature to evaluate whether non-invasive urine sampling is currently exploited to its full potential in the development of diagnostic tools for human helminthiases. Though further development, assessment and validation are needed before their routine use in control programmes, low-cost, rapid and reliable assays capable of detecting transrenal helminthderived antigens and cell-free DNA show excellent promise for future use at the point-ofcare in high-, medium-and even low-endemicity elimination settings.

show abstract

Relationship between eosinophil cationic protein and infection intensity in a schistosomiasis endemic community in Ghana

Cited by 2 publications

References 21 publications

Study of eosinophil cationic protein serum levels in patients with toxocariasis

Study of eosinophil cationic protein serum levels in patients with toxocariasis

An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?

Contact Info

Product

Resources

About