Relationship between expression pattern of vitamin D receptor, 1 alpha‐hydroxylase enzyme, and chemokine RANTES genes and selected serum parameters during transition period in Holstein dairy cows

Abstract

ObjectivesThe objective of the present study was to evaluate the pattern of genetic expression of vitamin D receptor (VDR), 1 alpha-hydroxylase (1α-OHase) enzyme and chemokine regulated on activation normal T-cell expressed and secreted (RANTES) in peripheral blood of Holstein dairy cows during transition period.MethodsBlood samples were collected from 16 Holstein dairy cows at 3 weeks prior expected date of delivery (EDD), at the day of parturition, and 3 weeks post-partum for assessment of expression profile of studied genes using real-time PCR and measurement of glucose, ionized calcium (Ca), parathyroid hormone (PTH), inorganic phosphorous (P), sodium (Na), potassium (K), chloride (Cl), and magnesium (Mg) levels.ResultsCompared with 3 weeks prior EDD, VDR gene expression decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The genetic expression of 1α-OHase enzyme as well as PTH, K, Na and Cl levels increased significantly at the day of parturition. The Ca level decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The P level increased significantly at the day of parturition then decreased significantly at 3 weeks post-partum. Glucose level decreased significantly at the day of parturition and at 3 weeks post-partum. RANTES gene expression showed non-significant changes among the three different time points. The expression of VDR gene had a negative correlation with the expression of 1α-OHase enzyme gene, and serum levels of glucose, PTH, P and K, but had a positive correlation with the serum Ca level. The expression of 1α-OHase enzyme gene had a positive correlation with serum levels of PTH, P and K, but had a negative correlation with the serum Ca level.ConclusionsResults of the current study indicate the importance of monitoring the genetic expression of VDR and 1α-OHase enzyme as indicators of metabolic changes during transition period, suggesting that they are candidate genes to judge the health status of dairy cows during such period.

DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes

“…Additionally, in contrast to cows with sub-clinical endometritis, the gene expression profiles of C3 , CXCL8 , LTF , TLR2 , and TRAPPC13 were temporally changed in healthy cows’ circulating WBC during the postpartum period [ 57 ]. CCL5 gene expression did not significantly alter between the three different time periods during the transition phase [ 18 ]. In goats, the LTF gene for mastitis resistance was discovered to have an up-regulated expression profile in Damascus goats [ 58 ].…”

“…It was previously believed that problems with general health at this time had a secondary impact on immunological response. However, when new cellular and molecular immunological pathways emerged, these issues and how they affected the immune system started to assume a more definite molecular reality [ 18 ]. Innate immunity supports host genetic resistance primarily, providing defense against pathogens without vaccination or exposure to illnesses [ 19 ].…”

MATERIALS of the International Scientific Conference "Actual issues of veterinary medicine", dedicated to the 100th anniversary of the Department of Clinical Diagnostics, Internal Diseases of Animals. Sineva A.V., obstetrics

“…Роль кальция и фосфора, а также витамина D в организме молочных коров хорошо изучена и достаточно полно освещена в отечественной и зарубежной как зоотехнической, так и ветеринарной литературе [3,5,16]. Снижение уровня кальция в крови приводит к нарушению многих функций органов и систем организма крупного рогатого скота [21,22,25,27,28,30,32,34,35].…”

Predictive biochemical and oxidative markers for dairy cows with and without retained fetal placenta

“…Xiao et al, (1998) found that in cows, E2 secretion is highest at estrus thus; the greatest expression of VEGF mRNA expression at estrus may be stimulated by E2 Saed et al, (2020). elicited that prior to parturition by 3 weeks the expression level of RANTES gene increased while at time of parturition the expression of the gene was down regulated Porwal et al, (2021).…”