Relationship between gametic differentiation and the cell cycle in the green alga Chlamydomonas eugametos

Zachleder, Vilém; Jakobs, Marja E.; Ende, H. van den

doi:10.1099/00221287-137-6-1333

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…A gametogenic division occurred just before the culture entered stationary phase (Tomson et af., 1985). Recently, zoospores of C. eugametos were described to behave like gametes (Zachleder et al, 1991). The gametic stage was suggested to be a regular part of the cell cycle in synchronous exponen tial-p hase cultures, which were supposedly growing under conditions without nutrient limitation.…”

Section: Mating Competence and Agglutinin Productionmentioning

confidence: 99%

“…Interestingly, the onset of excretion of extracellular material coincided with the decrease in growth of the cells. The production of this material preceded the retraction of the flagella and mitosis (Zachleder et al, 1991). Fig.…”

Section: Characterization Of the Cell Cyclementioning

confidence: 99%

“…C . eugametos pass through a phase of mating competence as a regular part of the cell cycle (Zachleder et al, 1991). To investigate this further, young daughter cells at 24 h were compared to gametes obtained by flooding plate cultures.…”

Section: Mating Competence and Agglutinin Productionmentioning

confidence: 99%

“…It was recently shown in our laboratory that in synchronous cultures of C. eugametos, daughter cells behave like gametes for some hours after being released from the mother cell (Zachleder et al, 1991). It was therefore proposed that a gamete phase forms a regular part of the cell cycle in C. eugametos.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the cell cycle in synchronous cultures of Chlamydomonas eugametos in relation to gametogenesis

Molendijk

Egmond

Haring

et al. 1992

Journal of General Microbiology

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The cell cycle of Chlamydomonas eugmetos was synchronized at low cell densities by growing cells under conditions of alternating light and dark periods. Synchronization of the cell cycle was demonstrated by determining cell size, cytokinesis, and release of daughter cells. The microscopic data were in agreement with the accumulation patterns of mRNAs for ribulose bisphosphate carboxylase/oxygenase small subunit, histone H4 and P-tubulin. Cells of C. eugametos secrete a sticky material which enables cells to adhere to a solid substrate during cell division. The secretion of this material was also cell-cycle-regulated and preceded cytokinesis. Recently, it was reported that in synchronous exponential-phase cultures of high cell densities, young daughter cells temporarily behave like gametes during the cell cycle. However, under our growth conditions this was not observed. First, daughter cells did not agglutinate with normal gametes of the opposite mating type. Second, extraction of growing cells did not release any agglutinin activity as measured both by an in uitro agglutination assay and by an agglutinin-specific monoclonal antibody. We conclude therefore that gametogenesis in C. eugametos is not necessarily a normal phase of the cell cycle.

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Section: Mating Competence and Agglutinin Productionmentioning

confidence: 99%

Section: Characterization Of the Cell Cyclementioning

confidence: 99%

Section: Mating Competence and Agglutinin Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of the cell cycle in synchronous cultures of Chlamydomonas eugametos in relation to gametogenesis

Molendijk

Egmond

Haring

et al. 1992

Journal of General Microbiology

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“…They showed that synchronized cells transferred to N-free conditions in early G1 became mating competent directly, but cells transferred 6 or 12 h later needed a cell division before H. van den Ende Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands; Fax no: +31-20-525-7934 producing gametes. Also, synchronous cells of C. eugametos showed mating competence only during the first part of the cell cycle (Zachleder et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Sexual development in the homothallic green alga Chlamydomonas monoica Strehlow

Ende

1995

Sexual Plant Reprod

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Sexual development in the homothallic green alga Chlamydomonas monoica strehlow van den Ende, H. Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Abstract In Chlamydomonas monoica, cell division and mating are interdependent processes, since under gametogenic conditions only newly born cells are mating competent. By refeeding nitrogen-starved cells with nitrate or ammonium ions, cell division and mating were synchronized. The mating competence of the progeny cells was dependent on the amount of the nitrogen source parent cells were refed, with an optimum around 0.1 btmol.105 cells. A second treatment with nitrate inhibited gametogenesis, but only when applied during the first part of the cell cycle, suggesting that an essential part of sexual development takes place during this period. During the latter part of the cell cycle, cells required light to acquire mating competence.

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The importance of light and photoperiod in sexual reproduction and geographical distribution in the green snow alga,Chloromonas sp.-D (Chlorophyceae, Volvocales)

et al. 2000

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The effects of premating light regimes on sexual reproduction and the production of spherical cells in Chloromonas sp.-D, a unicellular green snow alga, were studied using cross-mating strains 582C and 582D isolated from snowpacks associated with mixed hardwood-softwood forests in Whetstone Gulf State Park, Tughill Plateau, NY. Two preacclimation regimes were used, Vita-Lite as controls (530-700 nm peak) and blue light as experimentals (430-460 nm peak) prior to the mating experiments. In blue light, an increase in the number of matings and spherical cells (spheres) produced in the life cycle was observed as the photoperiod increased. This implies that longer photoperiods of blue light are more optimal for sexual reproduction in Chloromonas sp.-D than shorter ones. Under Vita-Lite, there was a significant increase in the number of matings and spheres with the extended 20 : 4 photoperiod compared with the shorter 14 : 10 photoperiod. Under blue light, significantly more matings and spheres occurred than under Vita-Lite using the same irradiance level of 95 µmol photons m 2 s 1 (photosynthetically active radiation [PAR] of 400-700 nm) for the 14 : 10 and 20 : 4 photoperiods. The results of these experiments suggest that Chloromonas sp.-D, known only from the Tughill Plateau, NY, is not reproducing optimally at this site where it grows and reproduces under an approximate 14 : 10 photoperiod in early April. However, in the upper 10 cm of snow in the Tughill Plateau, a blue light irradiance level of 95 µmol photons m 2 s 1 occurs, which is optimal for this species. When these conditions are combined with a 14 : 10 photoperiod, the Tughill Plateau appears to be sub-optimal for mating and production of spherical cells. Since Chloromonas sp.-D does not appear to have a dependence on a dark cycle, this would allow it to expand its geographical distribution. It may reproduce more optimally under blue light (95 µmol photons m 2 s 1 ) with an extended photoperiod (>20 : 4 h, light:dark) in high latitude field sites such as Lake Bienville, Québec, in eastern North America where other species of Chloromonas are known in snow associated with coniferous forests. Copyright

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Relationship between gametic differentiation and the cell cycle in the green alga Chlamydomonas eugametos

Cited by 15 publications

References 19 publications

Characterization of the cell cycle in synchronous cultures of Chlamydomonas eugametos in relation to gametogenesis

Characterization of the cell cycle in synchronous cultures of Chlamydomonas eugametos in relation to gametogenesis

Sexual development in the homothallic green alga Chlamydomonas monoica Strehlow

The importance of light and photoperiod in sexual reproduction and geographical distribution in the green snow alga,Chloromonas sp.-D (Chlorophyceae, Volvocales)

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