Relationship between glutathione levels and drug or radiation sensitivities in human gastric cancer cell lines in vitro

Abstract

Permanent cell lines and clones established from an untreated patient (AGS cells) with gastric carcinoma, and from a similar patient who had been treated with Adriamycin, 5FU and cytoxan (SII cells) were used in a study that compared their drug and radiation survival sensitivities to their glutathidine (GSH) values. The SII parental cell line was more resistant than the AGS cells in vitro to chlorambucil, ACT D, Adria, Bleo, and X-rays. This greater resistance was positively correlated with GSH values that were 1.77 times higher than in the AGS parental cell line. By contrast the SII parental cells were more sensitive than the AGS cells to MeCCNU and Melphalan. The drug and radiation sensitivities expressed among the clones of the two cell lines were heterogeneous and did not correlate with their GSH values.

Suppressing Wnt Signaling by the Hedgehog Pathway through sFRP-1

“…Cell Culture and Plasmids-The SIIA cell line was established in the Department of Surgery in our institution (23). AGS is another cell line from gastric adenocarcinomas used in this study.…”

Bombesin stimulates the in vitro growth of a human gastric cancer cell line

“…Cell culture The SIIA cell line was established in our laboratory from a gastric adenocarcinoma of a patient who underwent operative resection of this tumor (Barranco et al, 1991). SIIA cells were grown in F10 medium (Gibcoi BRL, Grand Island, NY) supplemented with 5% fetal calf serum (FCS; Hyclone, Logan, UT) in a humidified atmosphere with 95% air and 5% CO, a t 37°C.…”

Suppression of distant pulmonary metastasis of MDA-MB 435 human breast carcinoma established in mammary fat pads of nude mice by retroviral-mediated TIMP-2 gene transfer

“…On day 3, the medium was replaced with 10 ml of fresh medium. For assay of virus carrying the neo-selectable marker, AGS cells [39] were plated on six-well dishes, 1 × 10 5 cells per well, in 3 ml of medium on day 3. On day 4, the medium was changed to 1 ml of serial-diluted supernatant sample containing 8 µg/ml Polybrene.…”