Relationship between haematological profile and progression or spontaneous regression of melanoma in the Melanoma-bearing Libechov Minipigs

Čížková, Jana; Erbanová, M.; Sochor, Jiří­; Jindrová, Andrea; Strnadova, Kristyna; Horák, Vratislav

doi:10.1016/j.tvjl.2019.04.016

Cited by 4 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, melanoma-bearing MeLiM pigs were reported to show white blood cell counts not significantly different from control MeLiM pigs, neutrophils instead were more abundant in sick ones (36). Here we confirm this effect on circulating neutrophils on growing animals.…”

Section: Discussionsupporting

confidence: 85%

The Composition of Circulating Leukocytes Varies With Age and Melanoma Onset in the MeLiM Pig Biomedical Model

et al. 2020

View full text Add to dashboard Cite

Immunological research in pigs benefits from many improvements with a direct impact on the veterinary control of pig husbandry and on biomedical models. We compiled the available knowledge to develop gating strategies to monitor simultaneously all blood immune cell types by multicolor flow cytometry in Melanoblastoma-bearing Libechov Minipigs (MeLiM). The MeLiM pig spontaneously develops cutaneous melanomas that regress few months later. We monitored lymphoid and myeloid cell subsets in 3 to 21 weeks old pigs. Interestingly, neutrophils, type III monocytes (CD163 + CD14 + MHC II −) and CD4 − CD8α − T cells are less abundant in oldest animals in contrast to eosinophils, type II monocytes (CD163 − CD14 low MHC II +), B cells, γδ T cells, CD4 + CD8α + and CD4 − CD8α + T cells. Melanoma occurrence led to changes in the blood cell composition. Higher proportions of NK cells, CD4 + and CD4 + CD8α + T cells, and CD21 − B cells among B cells are found in young melanoma-bearing piglets, consistent with the immune-mediated spontaneous regression in the MeLiM model.

show abstract

Section: Discussionsupporting

confidence: 85%

The Composition of Circulating Leukocytes Varies With Age and Melanoma Onset in the MeLiM Pig Biomedical Model

et al. 2020

View full text Add to dashboard Cite

show abstract

“…22,28 Although spontaneous regression of melanoma has not been reported in dogs, there are references to this event in humans and swine, suggesting that this tumor may be susceptible to immune responses. 3,7,8,18 The presence of an inflammatory infiltrate, including tumorinfiltrating lymphocytes (TILs) in tumor biopsies, is evidence that the immune system responds to tumors. The term TILs was first used by Wallace H. Clark Jr in a study of cutaneous human melanomas, who developed a classification system for the distribution pattern of TILs in melanomas.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Intratumoral T-Lymphocyte Subsets in Canine Oral Melanoma and Their Association With Clinical and Histopathological Parameters

et al. 2021

View full text Add to dashboard Cite

Canine oral melanoma is a common, aggressive tumor with limited treatment options. Tumor-infiltrating lymphocytes (TILs) are important in antitumor immunity. This study used histopathology and immunophenotyping by flow cytometry to evaluate the presence and distribution of TILs in canine oral melanoma, including the frequency of CD8+ T cells, CD4+ T cells, and regulatory T cells. Fifty samples of oral melanoma from 45 dogs that did not receive treatment prior to surgery were included in the study. The distribution of TILs in the tissue (brisk, nonbrisk, and absent) was evaluated in 48 samples. Twenty-eight (58%) samples had a brisk distribution pattern, 10 (21%) samples had a nonbrisk pattern, and 10 (21%) samples had an absent TIL pattern. Comparing the histological evaluation and the immunophenotyping data, it was observed that samples with a brisk TIL pattern had a higher frequency of CD8+ T lymphocytes ( P = .05) and a lower frequency of CD4+/CD25+/FoxP3+ Tregs ( P = .03), compared to the samples with nonbrisk and absent infiltrate patterns. Patients with a higher survival rate had higher TIL scores ( P = .002), a brisk or nonbrisk TIL pattern ( P = .001), and an increased frequency of CD8+ T lymphocytes infiltrating the tumor ( P = .003). Our analysis suggests that the evaluation of TILs in canine oral melanoma is relevant to predict tumor aggressiveness and patient prognosis.

show abstract

Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model

et al. 2021

View full text Add to dashboard Cite

Melanoma-bearing Libechov minipig (MeLiM) represents a large animal model for melanoma research. This model shows a high incidence of complete spontaneous regression of melanoma – a phenomenon uncommon in humans. Here, we present the first metabolomic characterisation of the MeLiM model comparing animals with progressing and spontaneously regressing melanomas. Plasma samples of 19 minipigs with progression and 27 minipigs with evidence of regression were analysed by a targeted metabolomic assay based on mass spectrometry detection. Differences in plasma metabolomics patterns were investigated by univariate and multivariate statistical analyses. Overall, 185 metabolites were quantified in each plasma sample. Significantly altered metabolomic profile was found, and 42 features were differentially regulated in plasma. Besides, the machine learning approach was used to create a predictive model utilising Arg/Orn and Arg/ADMA ratios to discriminate minipigs with progressive disease development from minipigs with regression evidence. Our results suggest that progression of melanoma in the MeLiM model is associated with alteration of arginine, glycerophospholipid and acylcarnitines metabolism. Moreover, this study provides targeted metabolomics characterisation of an animal model of melanoma with progression and spontaneous regression of tumours.

show abstract

Relationship between haematological profile and progression or spontaneous regression of melanoma in the Melanoma-bearing Libechov Minipigs

Cited by 4 publications

References 44 publications

The Composition of Circulating Leukocytes Varies With Age and Melanoma Onset in the MeLiM Pig Biomedical Model

The Composition of Circulating Leukocytes Varies With Age and Melanoma Onset in the MeLiM Pig Biomedical Model

Intratumoral T-Lymphocyte Subsets in Canine Oral Melanoma and Their Association With Clinical and Histopathological Parameters

Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model

Contact Info

Product

Resources

About