Relationship between hyposalivation and oxidative stress in aging mice

Yoda, Yoshitaka; Matsuno, Tomonori; Omata, Kazuhiko; Satoh, Tazuko

doi:10.3164/jcbn.16-79

Cited by 42 publications

(36 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As that of physiological aging, the periodontitis leads to an increase in the buildup of oxidative stress causing structural changes. 18 This study might be the first to show the periodontal disease effect on salivary glands and its flow like the best of our knowledge.…”

Section: Discussionmentioning

confidence: 70%

Correlation of Unstimulated and Stimulated Salivary Flow Rate in Chronic Periodontitis

Alasqah¹

2019

World Journal of Dentistry

View full text Add to dashboard Cite

Aim: The present study aimed at correlating the flow rate of the stimulated and unstimulated saliva in chronic periodontitis. Materials and methods:This study composed of 100 subjects between the age group of 25 years and 45 years. These subjects are categorized into four groups, placing 25 subjects in each. Group 1 with 25 healthy patients (no loss of attachment clinically), group 2 with 25 mild chronic periodontitis subjects (1-2 mm of attachment level clinically), group 3 with 25 moderate chronic periodontitis subjects (3-4 mm of attachment level clinically), group 4 with 25 severe chronic periodontitis subjects (5 mm of attachment level clinically), split method was used to collect the stimulated and unstimulated saliva. A calibrated digital balance was used to weigh the bottle before and after the collection of saliva. The result was considered to be statistically significant at a p value less than 0.05 (p <0.05). Result:The stimulated and unstimulated flow rate of saliva was high in group 1 subjects (0.920 ± 0.240, 1.366 ± 0.280) followed by group 2 subjects (0.780 ± 0.246, 0.920 ± 0.146), group 3 subjects (0.408 ± 0.132, 0.590 ± 0.110), and group 4 subjects (0.221 ± 0.348, 0.301 ± 0.216). There was a significant difference in the salivary flow rate between the groups statistically with p value 0.0001. Conclusion:The stimulated and unstimulated salivary flow rate decreased with the severity of the progression of the chronic periodontitis. Clinical significance:The importance of saliva on oral function includes antimicrobial activity, buffering function of pH, cleansing action, mastication, and swallowing. Therefore, a decrease in the ability to produce saliva leads to various oral infections.

show abstract

Section: Discussionmentioning

confidence: 70%

Correlation of Unstimulated and Stimulated Salivary Flow Rate in Chronic Periodontitis

Alasqah¹

2019

World Journal of Dentistry

View full text Add to dashboard Cite

show abstract

“…It has been shown that in species in which females have a shorter life span than males, females produce more free radicals (Viña et al 2013). A study has shown that aging mice have higher concentrations of oxidative stress markers and lower antioxidant status in saliva (Yamauchi et al 2017). The majority of animal experiments are performed using young to middle aged animals.…”

Section: Discussionmentioning

confidence: 99%

Markers of Oxidative Stress and Antioxidant Status in the Plasma, Urine and Saliva of Healthy Mice

Gyurászová

Kovalčíková²,

Janšáková³

et al. 2018

Physiol Res

View full text Add to dashboard Cite

Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, healthy mice and to identify some sources of variability. Samples were obtained from 41 female and 37 male adult, healthy mice of the CD-1 strain, aged 95-480 days, weighing 21-55 grams.Reference ranges of TBARS (thiobarbituric acid reactive substances), AOPP (advanced oxidation protein products), fructosamine, GSH/GSSG (reduced and oxidized glutathione) ratio, TAC (total antioxidant capacity), and FRAP (ferric reducing antioxidant power) were measured in plasma and urine, and TBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standard spectrophotometric and fluorometric methods. Salivary GSH/GSSG and urinary AOPP were higher in females. Urinary fructosamine, GSH/GSSG and FRAP were higher in males. Urinary TAC and FRAP negatively correlated with age, and urinary GSH/GSSG positively correlated with weight. We determined that urine and saliva can be obtained non-invasively from mice, in sufficient amounts for reliable oxidative status assessment.Further studies are needed to uncover whether these biofluids reflect systemic oxidative status in diseases.

show abstract

“…Furthermore, it is believed that oxidative stress leads to salivary gland damage associated with aging [ 3 ]. The presence of anatomical and morphological changes has been demonstrated in salivary gland parenchyma of older individuals, which leads to impaired salivary flow, a decrease in its buffering capacity, the weakening of immune response mechanisms and disturbances in the qualitative composition of saliva, which may result from the cellular accumulation of DNA, protein and lipid oxidation products [ 3 , 25 , 26 ]. With advancing age, a significant reduction in the effectiveness of enzymatic and non-enzymatic antioxidant defense systems also occurs [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

Whey Protein Concentrate WPC-80 Improves Antioxidant Defense Systems in the Salivary Glands of 14-Month Wistar Rats

et al. 2018

View full text Add to dashboard Cite

Whey protein concentrate (WPC) is characterized by powerful antioxidant properties, but its effect on redox homeostasis of salivary glands of aging organisms is still unknown. In this study, we are the first to evaluate the antioxidant barrier of salivary glands of 14-month Wistar rats fed WPC-80. Total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) as well as concentrations of reduced glutathione (GSH) are estimated in the submandibular and parotid glands of rats administered WPC-80 intragastrically for a period of 7 and 14 days. We demonstrate a significant increase in GSH, GPx and SOD in the salivary glands of rats fed WPC-80 for 14 days and a significant increase in TAS, GPx and SOD in the parotid glands of rats fed WPC-80 for 7 days compared to control rats. The beneficial effects of WPC-80 on salivary glands are also demonstrated by lower TOS and OSI in the parotid glands of rats fed WPC-80 compared to the submandibular glands. In summary, we demonstrate that WPC-80 improves redox homeostasis in salivary glands, particularly in the parotid glands of old rats.

show abstract

Relationship between hyposalivation and oxidative stress in aging mice

Cited by 42 publications

References 39 publications

Correlation of Unstimulated and Stimulated Salivary Flow Rate in Chronic Periodontitis

Correlation of Unstimulated and Stimulated Salivary Flow Rate in Chronic Periodontitis

Markers of Oxidative Stress and Antioxidant Status in the Plasma, Urine and Saliva of Healthy Mice

Whey Protein Concentrate WPC-80 Improves Antioxidant Defense Systems in the Salivary Glands of 14-Month Wistar Rats

Contact Info

Product

Resources

About