Relationship between IgE positive cell numbers and serum total IgE levels in mice treated with trimellitic anhydride and dinitrochlorobenzene

Ban, M.; Hettich, D.

doi:10.1016/s0378-4274(00)00280-0

Cited by 26 publications

(15 citation statements)

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“…Total serum IgE was measured using ELISA as described previously (Ban and Hettich, 2001). Briefly, 96-well microimmunoplates (NUNC) were incubated with a 2 g/mL rat monoclonal anti-mouse IgE antibody overnight at 4 • C, washed four times with 0.1 M phosphate-buffered saline (PBS) containing 0.05% Tween-20 (PBS-T) and incubated successively with (1) 100 L per well PBS containing 1% serum albumin bovine (BSA) 10% fetal calf serum (blocking buffer), (2) 100 L per well mouse serum diluted 1:3 in PBS or mouse antitrintrophenol IgE standard, (3) 100 L per well rat anti-mouse IgE antibody labelled with peroxidase.…”

Section: Elisa For Total Serum Igementioning

confidence: 99%

TDI can induce respiratory allergy with Th2-dominated response in mice

et al. 2006

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Section: Elisa For Total Serum Igementioning

confidence: 99%

TDI can induce respiratory allergy with Th2-dominated response in mice

et al. 2006

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“…Total serum IgE was measured by ELISA as described previously (Ban and Hettich, 2001). Briefly, 96-well microimmunoplates (Nunc) were incubated with 2 µg ml −1 rat monoclonal anti-mouse IgE antibody overnight at 4°C, washed four times with 0.1 M phosphate-buffered saline (PBS) containing 0.05% Tween-20 (PBS-T) and incubated successively with: 100 µl per well of PBS containing 1% bovine serum albumin (BSA) and 10% foetal calf serum (blocking buffer); 100 µl per well of mouse serum diluted 1 : 3 in PBS or mouse anti-trinitrophenol IgE standard; and 100 µl per well of rat anti-mouse IgE antibody labelled with peroxidase.…”

Section: Serum Igementioning

confidence: 99%

“…In a previous mice study, treatment with trimellitic anhydride (TMA) and phthalic anhydride (PA), which are known in humans as respiratory allergens, provoked a strong Th2 immune response associated with increases of total serum concentrations of IgE and of IgE positive cells in the respiratory tract (Ban and Hettich, 2001). There is accumulating evidence that Th2 cytokines such as IL-4, IL-5 and IL-13 promote Th2-cell differentiation and B-cell-dependent IgE production and that these responses are down-regulated by Th1 cytokines such as IFN-γ and IL-12 (Gavett et al, 1995;Lack et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Effect of Th2 cytokine antagonist treatments on chemical-induced allergic response in mice

Ban

Hettich

2005

J. Appl. Toxicol.

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To understand better the cellular and molecular mechanisms of chemical-induced occupational asthma, we examined the effects of the Th2 cytokine antagonists interferon-gamma (IFN-gamma), interleukin (IL)-12 and anti-IL-4 on the balance of the Th1/Th2 response induced by trimellitic anhydride (TMA) and phthalic anhydride (PA). Eight- to ten-week-old BALB/c mice were assigned to be exposed to either TMA or PA plus one of these Th2 cytokine antagonists. Both TMA (25% and 12.5% for sensitization and challenge, respectively) and PA (12.5% and 6.25% for sensitization and challenge, respectively) induced a Th2 response marked by an increasing production of IL-4 and IL-10 in the supernatants of ex vivo spleen cells cultured with concanavalin A and also of serum total IgE. Co-administration of IL-12 and antiIL-4 deviated these PA- or TMA-induced Th2 responses, as judged by an increasing serum total IgG2a production (up to 14-fold), associated with a slight decrease of IL-4 in three out of four experiments and of IL-10 in all four experiments. Co-administration of IFN-gamma, however, had only one weak effect. These findings suggest that the chemical-induced Th2-biased response may be diverted during an induction period by exogenous administration of the Th2 cytokine antagonists, particularly IL-12 and the anti-IL-4 antibody. These results would significantly enhance our understanding of the Th1/Th2 response induced by chemicals.

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“…2). Previous reports also showed that dermal application of not only respiratory sensitizers but additionally a contact sensitizer DNCB increased serum total IgE in mice (Ban and Hettich, 2001;Selgrade et al, 2006). While serum IgE is considered to be one of the triggers of chemical-induced asthma (Holsapple et al, 2006;Arts and Kuper, 2007), epidemiological studies have suggested that IgE independent responses may also be involved .…”

Section: Discussionmentioning

confidence: 98%