Relationship between Immune Function and Serum Vitamin A in Japanese Black Beef Cattle

Yano, Hiraku; Ohtsuka, Hiromichi; Miyazawa, Motoki; Abiko, Shigeki; Ando, Takaaki; Watanabe, Daisaku; Matsuda, Kazuo; Kawamura, Seiichi; Arai, Toshiro; Morris, S. T.

doi:10.1292/jvms.71.199

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…It was suggested that optimal marbling scores are obtained when animals are kept very close to health deteriorating low vitamin A plasma concentrations in a range of 100–150 μg/L (40–50 IU/dl) over a period of usually 12 month during the fattening period [16]. However, in Japanese Black cattle low vitamin A plasma concentrations have been reported to be associated with an impaired immune function [17] and increased morbidity and mortality [18]. Therefore, blood vitamin A levels in Japanese Black cattle needs to be carefully monitored and nutritionally controlled.…”

Section: Introductionmentioning

confidence: 99%

Validation of blood vitamin A concentrations in cattle: comparison of a new cow-side test (iCheck™ FLUORO) with high-performance liquid chromatography (HPLC)

et al. 2017

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BackgroundPlasma concentration of retinol is an accepted indicator to assess the vitamin A (retinol) status in cattle. However, the determination of vitamin A requires a time consuming multi-step procedure, which needs specific equipment to perform extraction, centrifugation or saponification prior to high-performance liquid chromatography (HPLC).MethodsThe concentrations of retinol in whole blood (n = 10), plasma (n = 132) and serum (n = 61) were measured by a new rapid cow-side test (iCheck™ FLUORO) and compared with those by HPLC in two independent laboratories in Germany (DE) and Japan (JP).ResultsRetinol concentrations in plasma ranged from 0.033 to 0.532 mg/L, and in serum from 0.043 to 0.360 mg/L (HPLC method). No significant differences in retinol levels were observed between the new rapid cow-side test and HPLC performed in different laboratories (HPLC vs. iCheck™ FLUORO: 0.320 ± 0.047 mg/L vs. 0.333 ± 0.044 mg/L, and 0.240 ± 0.096 mg/L vs. 0.241 ± 0.069 mg/L, lab DE and lab JP, respectively). A similar comparability was observed when whole blood was used (HPLC vs. iCheck™ FLUORO: 0.353 ± 0.084 mg/L vs. 0.341 ± 0.064 mg/L). Results showed a good agreement between both methods based on correlation coefficients of r2 = 0.87 (P < 0.001) and Bland-Altman blots revealed no significant bias for all comparison.ConclusionsWith the new rapid cow-side test (iCheck™ FLUORO) retinol concentrations in cattle can be reliably assessed within a few minutes and directly in the barn using even whole blood without the necessity of prior centrifugation. The ease of the application of the new rapid cow-side test and its portability can improve the diagnostic of vitamin A status and will help to control vitamin A supplementation in specific vitamin A feeding regimes such as used to optimize health status in calves or meat marbling in Japanese Black cattle.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1042-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Validation of blood vitamin A concentrations in cattle: comparison of a new cow-side test (iCheck™ FLUORO) with high-performance liquid chromatography (HPLC)

et al. 2017

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“…The prevalence of EPP has increased throughout Japan since late 1990s when VA-deficient feed was recommended by many prefectural governors ( 1 ). VA deficiency has been reported to increase sensitivity to allergy with a Th2 shift in JB cattle ( 24 ) or laboratory mice ( 25 ). VA deficiency has been reported to increase the risk of Treg-to-Type 17 helper T cell (Th17) reprogramming, resulting in the induction of enteritis ( 26 ) and IgE-mediated food allergy ( 27 ); that is, it was suggested to enhance allergic responses via Th17 because Treg with the immunosuppressive function is reduced by VA deficiency.…”

Section: Discussionmentioning

confidence: 99%

Intrahepatic eosinophilic proliferative phlebitis in Japanese black cattle indicate allergies involving mast cell tryptase-dependent activation

et al. 2022

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Cow-specific feature hepatic lesion, termed as eosinophilic proliferative phlebitis (EPP), has been mainly detected in Japanese black cattle and identified histologically eosinophilic infiltration and endothelial hyperplasia in portal areas. We previously proposed EPP as a food allergy from the pathological characteristics and a significant increase of serum immunoglobulin E specific to curly dock (Rumex crispus) in allergens testing, however, first report had regarded EPP an atypical type of bovine fascioliasis. In EPP lesions, eosinophilic infiltration was observed to the hypertrophic endothelium and not to the intrahepatic bile duct, and that was related to eotaxin-1 expression. In EPP, the mast cells increased as well as in fascioliasis, and the mast cells producing tryptase without chymase increased with interleukin-4 production. In this context, hyperplasia of periendothelium expressing proteinase-activated receptor-2 (PAR-2) and not angiotensin II was observed. Contrastably, in fascioliasis, unique mast cells producing neither tryptase nor chymase infiltrated, and the periendothelium expressed neither PAR-2 nor angiotensin II. Interestingly, EPP had not occurred liver injury with raised hepatic enzymes like fascioliasis, and suggested to a correlation with severe serum hypo-vitamin A. Overall, this study suggests that EPP is an allergic disease by main difference between adaptive immunity to allergens and innate immunity to parasites.

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“…As a result, stringent control of retinol levels in the blood cannot be achieved, representing a possible reduction in the price of the dressed carcass due to low marbling. Controlling retinol at physiological limits also indicates possible deficiency, leading to immunodeficiency and death (Adachi et al, 1997 ; Yano et al, 2009 ), with obvious negative implications for farm income and management.…”

Section: Introductionmentioning

confidence: 99%

Preliminary system of rapid analysis of blood retinol level in cattle

Iwasaki

Morita

Inoue³

et al. 2021

Animal Science Journal

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Control of blood retinol levels in cattle during fattening is important in the production of marbled beef. However, it is difficult to easily measure the blood retinol concentration in the field. In this study, we attempted to develop an analysis method that does not require blood cell separation and uses a compact fluorescence analyzer that can be carried around as a preliminary system for measuring blood retinol concentration in the field. This system was used to monitor blood retinol levels in 12 fattening cattle (14 to 27 months old) and demonstrated a strong correlation (r = 0.78) with the results obtained by the standard high‐performance liquid chromatography (HPLC) method. Stronger correlations (r = 0.87) were obtained until the cattle were 24 months of age. These results suggest that higher correlations can be expected to be obtained by improving the robustness of the extraction system. Refinements for practical use need to be considered, but whole blood extraction and the vitamin A analyzer that was developed show potential to be used for on‐farm monitoring of retinol levels.

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Relationship between Immune Function and Serum Vitamin A in Japanese Black Beef Cattle

Cited by 6 publications

References 23 publications

Validation of blood vitamin A concentrations in cattle: comparison of a new cow-side test (iCheck™ FLUORO) with high-performance liquid chromatography (HPLC)

Validation of blood vitamin A concentrations in cattle: comparison of a new cow-side test (iCheck™ FLUORO) with high-performance liquid chromatography (HPLC)

Intrahepatic eosinophilic proliferative phlebitis in Japanese black cattle indicate allergies involving mast cell tryptase-dependent activation

Preliminary system of rapid analysis of blood retinol level in cattle

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