Relationship Between Ischemia/Reperfusion Injury and the Stimulus of Fibrogenesis in an Experimental Model: Comparison Among Different Preservation Solutions

Camacho, Vera Regina Rodrigues; Fraga, Raquel Scherer de; Souza, Gabriela de Nardi; Cerski, Carlos Thadeu Schmidt; Oliveira, Jarbas Rodrigues de; Oliveira, Marcelo Ganzarolli de; Álvares‐da‐Silva, Mário Reis

doi:10.1016/j.transproceed.2011.08.114

Cited by 6 publications

(8 citation statements)

References 17 publications

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“…The same bloodlike fluid was used for 2 hours of ex vivo reperfusion of all rat livers, and the same perfusion system was used for NMP and ex vivo reperfusion. Previous studies have indicated that crystalloid fluids are sufficient for short‐term cold preservation of rat donor liver parenchyma . In a pilot study, we confirmed that rat donor liver preservation in either NaCl or histidine tryptophan ketoglutarate (HTK) solution (n = 6 in each group) does not result in a different degree of bile duct injury or function as measured by bilirubin, gamma‐glutamyltransferase (GGT), lactate dehydrogenase (LDH), pH, and HCO 3 − in bile produced during reperfusion (Supporting Text).…”

Section: Methodssupporting

confidence: 71%

“…Previous studies have indicated that crystalloid fluids are sufficient for short-term cold preservation of rat donor liver parenchyma. (17,18) In a pilot study, we confirmed that rat donor liver preservation in either NaCl or histidine tryptophan ketoglutarate (HTK) solution (n 5 6 in each group) does not result in a different degree of bile duct injury or function as measured by bilirubin, gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH), pH, and HCO 3 2 in bile produced during reperfusion (Supporting Text). For both NMP and ex vivo reperfusion of rat donor livers, we developed a liver machine perfusion system that enabled dual perfusion via both the hepatic artery and the portal vein in a closed circuit ( Fig.…”

Section: Scs and Nmpmentioning

confidence: 59%

“…The use of 0.9% NaCl for SCS preservation is a limitation of this study because it is not a commonly used preservation fluid for human livers. However, previous studies have demonstrated that crystalloid solutions such as NaCl 0.9% are sufficient preservation fluids for short‐term cold preservation of rodent livers . In a pilot study, we compared all biliary outcome parameters (bilirubin, GGT, LDH, pH, and HCO 3 − in bile produced during reperfusion) of 6 DCD livers statically preserved in HTK with 6 DCD livers preserved in NaCl 0.9%.…”

Section: Discussionmentioning

confidence: 99%

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Normothermic machine perfusion reduces bile duct injury and improves biliary epithelial function in rat donor livers

et al. 2016

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Bile duct injury may occur during liver procurement and transplantation, especially in livers from donation after circulatory death (DCD) donors. Normothermic machine perfusion (NMP) has been shown to reduce hepatic injury compared to static cold storage (SCS). However, it is unknown whether NMP provides better preservation of bile ducts. The aim of this study was to determine the impact of NMP on bile duct preservation in both DCD and non-DCD livers. DCD and non-DCD livers obtained from Lewis rats were preserved for 3 hours using either SCS or NMP, followed by 2 hours ex vivo reperfusion. Biomarkers of bile duct injury (gamma-glutamyltransferase and lactate dehydrogenase in bile) were lower in NMP-preserved livers compared to SCS-preserved livers. Biliary bicarbonate concentration, reflecting biliary epithelial function, was 2-fold higher in NMP-preserved livers (P < 0.01). In parallel with this, the pH of the bile was significantly higher in NMP-preserved livers (7.63 6 0.02 and 7.74 6 0.05 for non-DCD and DCD livers, respectively) compared with SCS-preserved livers (7.46 6 0.02 and 7.49 6 0.04 for non-DCD and DCD livers, respectively). Scanning and transmission electron microscopy of donor extrahepatic bile ducts demonstrated significantly decreased injury of the biliary epithelium of NMP-preserved donor livers (including the loss of lateral interdigitations and mitochondrial injury). Differences between NMP and SCS were most prominent in DCD livers. Compared to conventional SCS, NMP provides superior preservation of bile duct epithelial cell function and morphology, especially in DCD donor livers. By reducing biliary injury, NMP could have an important impact on the utilization of DCD livers and outcome after transplantation.

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Section: Methodssupporting

confidence: 71%

Section: Scs and Nmpmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

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Normothermic machine perfusion reduces bile duct injury and improves biliary epithelial function in rat donor livers

et al. 2016

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“…In our study, the "n = 9" was statistics by power analyses from preliminary experiment that used 2 groups of mean comparison sample cases formula. 32 From a statistical point of view, this sample was powered enough for pairwise comparisons of these parameters.…”

Section: Discussionmentioning

confidence: 99%

Antioxidation Role of Different Lateral Stellate Ganglion Block in Isoproterenol-Induced Acute Myocardial Ischemia in Rats

Wei

Chi

Deng

et al. 2017

Regional Anesthesia and Pain Medicine

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“…These procedures use various preservation materials, solutions, and/or chemicals to ensure that the entire organ remains viable and can resume its physiological functions after transplantation into the recipient’s body. However, the effects of the different types of solutions used for preservation/transportation on the cells and their influence on clinical efficacy in patients needs further extensive research 37,38. Preserving an organ which is a conglomeration of many different tissue types consisting of billions of cells, employing varied materials and methodologies of preservation for more than few hours is acceptable with limited or no studies of randomized trials on safety and efficacy.…”

Section: Discussionmentioning

confidence: 99%

Age-old wisdom concerning cell-based therapies with added knowledge in the stem cell era: our perspectives

Preethy¹,

John

Ganesh

et al. 2013

SCCAA

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Among the various strategies providing a cure for illness, cell-based therapies have caught the attention of the world with the advent of the “stem cell” era. Our inherent understanding indicates that stem cells have been in existence since the birth of multicellular organisms. However, the formal discovery of stem cells in the last century, followed by their intricate and extensive analysis, has led to clinical and translational efforts with the aim of using them in the treatment of conditions which don’t have a definitive therapeutic strategy, has fueled our interest and expectations. Technological advances in our ability to study their cellular components in depth, along with surface markers and other finer constituents, that were unknown until last century, have improved our understanding, leading to several novel applications. This has created a need to establish guidelines, and in that process, there are expressed understandings and views which describe cell therapy along lines similar to that of biologic products, drugs, and devices. However, the age-old wisdom of using cells as tools for curing illness should not be misled by recent knowledge, to make cell therapy using highly complex stem cells equal to factory-synthesized and reproducible chemical compounds, drugs, or devices. This article analyses the differences between these two entities from various perspectives.

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Relationship Between Ischemia/Reperfusion Injury and the Stimulus of Fibrogenesis in an Experimental Model: Comparison Among Different Preservation Solutions

Abstract: In this experimental model of early reperfusion injury, preservation changes related to higher levels of renin, which suggest its role in fibrogenesis. FBP was associated with lower renin levels than other solutions including UW.

Cited by 6 publications

References 17 publications

Normothermic machine perfusion reduces bile duct injury and improves biliary epithelial function in rat donor livers

Normothermic machine perfusion reduces bile duct injury and improves biliary epithelial function in rat donor livers

Antioxidation Role of Different Lateral Stellate Ganglion Block in Isoproterenol-Induced Acute Myocardial Ischemia in Rats

Age-old wisdom concerning cell-based therapies with added knowledge in the stem cell era: our perspectives

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