Relationship between L-lactate dehydrogenase and multidrug resistance in Staphylococcus xylosus

Yuan, Zhongwei; Wang, Jinpeng; Che, Ruixiang; Bello-Onaghise, God’spower; Zhou, Yonggang; Dong, Chunliu; Li, Lu; Chen, Mingri; Eliphaz, Nsabimana; Liu, Xin; Li, Yanhua

doi:10.1007/s00203-021-02625-8

Cited by 6 publications

(5 citation statements)

References 55 publications

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“…As S. epidermidis biofilms exposed to NO showed higher lactate accumulation, we investigated whether this increase could be related to the induced expression of ldh gene encoding the l -lactate dehydrogenase enzyme ( Figure 1 ). l -Ldh has been linked to drug resistance and is considered a possible target of regulatory control in many bacterial species, including in coagulase-negative staphylococci ( Richardson et al., 2008 ; Toyoda and Inui, 2021 ; Ma et al., 2022 ; Yuan et al., 2022 ). For this purpose, biofilms of strains 1457 and RP62A, treated with NO or left untreated and grown for 24 h, were used to extract total RNA and evaluate ldh expression by RT-qPCR.…”

Section: Resultsmentioning

confidence: 99%

Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress

Oliveira

Saraiva

Carvalho

2023

Front. Cell. Infect. Microbiol.

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Staphylococcus epidermidis is a commensal skin bacterium that forms host- and antibiotic-resistant biofilms that are a major cause of implant-associated infections. Most research has focused on studying the responses to host-imposed stresses on planktonic bacteria. In this work, we addressed the open question of how S. epidermidis thrives on toxic concentrations of nitric oxide (NO) produced by host innate immune cells during biofilm assembly. We analyzed alterations of gene expression, metabolism, and matrix structure of biofilms of two clinical isolates of S. epidermidis, namely, 1457 and RP62A, formed under NO stress conditions. In both strains, NO lowers the amount of biofilm mass and causes increased production of lactate and decreased acetate excretion from biofilm glucose metabolism. Transcriptional analysis revealed that NO induces icaA, which is directly involved in polysaccharide intercellular adhesion (PIA) production, and genes encoding proteins of the amino sugar pathway (glmM and glmU) that link glycolysis to PIA synthesis. However, the strains seem to have distinct regulatory mechanisms to boost lactate production, as NO causes a substantial upregulation of ldh gene in strain RP62A but not in strain 1457. The analysis of the matrix components of the staphylococcal biofilms, assessed by confocal laser scanning microscopy (CLSM), showed that NO stimulates PIA and protein production and interferes with biofilm structure in a strain-dependent manner, but independently of the Ldh level. Thus, NO resistance is attained by remodeling the staphylococcal matrix architecture and adaptation of main metabolic processes, likely providing in vivo fitness of S. epidermidis biofilms contacting NO-proficient macrophages.

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Section: Resultsmentioning

confidence: 99%

Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress

Oliveira

Saraiva

Carvalho

2023

Front. Cell. Infect. Microbiol.

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“…Bifidobacteria were not able to decarboxylate L-DOPA to dopamine, a pathway recently observed in Enterococcus and Eggerthella species 6 , 7 ; therefore, we searched for alternative pathways to explain our clinical observations. Non-commensals—including pathogens and environmental bacteria—frequently encode aromatic amino acid aminotransferases 26 – 28 that are possibly able to deaminate L-DOPA to DHPPA, and lactate dehydrogenases 8 , 29 , 30 that could reduce DHPPA to DHPLA. Indeed, a recent study found that the soil bacterium Clostridium sporogenes can efficiently deaminate L-DOPA to DHPPA using an aminotransferase 31 .…”

Section: Discussionmentioning

confidence: 99%

A novel pathway of levodopa metabolism by commensal Bifidobacteria

Cirstea,

Creus-Cuadros,

et al. 2023

Sci Rep

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The gold-standard treatment for Parkinson’s disease is levodopa (L-DOPA), which is taken orally and absorbed intestinally. L-DOPA must reach the brain intact to exert its clinical effect; peripheral metabolism by host and microbial enzymes is a clinical management issue. The gut microbiota is altered in PD, with one consistent and unexplained observation being an increase in Bifidobacterium abundance among patients. Recently, certain Bifidobacterium species were shown to have the ability to metabolize L-tyrosine, an L-DOPA structural analog. Using both clinical cohort data and in vitro experimentation, we investigated the potential for commensal Bifidobacteria to metabolize this drug. In PD patients, Bifidobacterium abundance was positively correlated with L-DOPA dose and negatively with serum tyrosine concentration. In vitro experiments revealed that certain species, including B. bifidum, B. breve, and B. longum, were able to metabolize this drug via deamination followed by reduction to the compound 3,4-dihydroxyphenyl lactic acid (DHPLA) using existing tyrosine-metabolising genes. DHPLA appears to be a waste product generated during regeneration of NAD +. This metabolism occurs at low levels in rich medium, but is significantly upregulated in nutrient-limited minimal medium. Discovery of this novel metabolism of L-DOPA to DHPLA by a common commensal may help inform medication management in PD.

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“…Although not reaching statistical significance, the common occurrence of S. xylosus, particularly among the organic farm isolates, was interesting as well ( Figure 1 , Table S1 ). As a NAS species, S. xylosus is gaining interest due to its increasing clinical appearance and frequent association with animal/human infection and serious multidrug resistance [ 41 , 42 , 43 , 44 ]. S. xylosus is a common commensal, originally isolated from the skin of animals and humans [ 45 , 46 ], and the species is reported to be frequently isolated from livestock and farm environments [ 47 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Farming Practice Influences Antimicrobial Resistance Burden of Non-Aureus Staphylococci in Pig Husbandries

et al. 2022

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Non-aureus staphylococci (NAS) are ubiquitous bacteria in livestock-associated environments where they may act as reservoirs of antimicrobial resistance (AMR) genes for pathogens such as Staphylococcus aureus. Here, we tested whether housing conditions in pig farms could influence the overall AMR-NAS burden. Two hundred and forty porcine commensal and environmental NAS isolates from three different farm types (conventional, alternative, and organic) were tested for phenotypic antimicrobial susceptibility and subjected to whole genome sequencing. Genomic data were analysed regarding species identity and AMR gene carriage. Seventeen different NAS species were identified across all farm types. In contrast to conventional farms, no AMR genes were detectable towards methicillin, aminoglycosides, and phenicols in organic farms. Additionally, AMR genes to macrolides and tetracycline were rare among NAS in organic farms, while such genes were common in conventional husbandries. No differences in AMR detection existed between farm types regarding fosfomycin, lincosamides, fusidic acid, and heavy metal resistance gene presence. The combined data show that husbandry conditions influence the occurrence of resistant and multidrug-resistant bacteria in livestock, suggesting that changing husbandry practices may be an appropriate means of limiting the spread of AMR bacteria on farms.

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Relationship between L-lactate dehydrogenase and multidrug resistance in Staphylococcus xylosus

Cited by 6 publications

References 55 publications

Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress

Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress

A novel pathway of levodopa metabolism by commensal Bifidobacteria

Farming Practice Influences Antimicrobial Resistance Burden of Non-Aureus Staphylococci in Pig Husbandries

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