Relationship between morphology and chromosomal constitution in human preimplantation embryo

Pellestor, Franck; Girardet, Anne; Andréo, Brigitte; Arnal, Françıoise; Humeau, C.

doi:10.1002/mrd.1080390204

Cited by 78 publications

(35 citation statements)

References 22 publications

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“…More studies have been performed to try to derive ES cells without destroying the fertilized embryos, including the use of poor-quality embryos, developmentally arrested embryos and even the blastomere biopsied embryos, and a relationship between morphology and chromosomal constitution in human pre-implantation embryos has been suggested. 34 Moreover, it has been reported that aneuploid chromosomes affect developmental competence and embryo quality, increase cell fragments and induce cell apoptosis, [35][36][37] which is in accordance with our results; furthermore, aneuploid chromosomes most likely also affected blastocyst formation and hES cell line derivation.…”

Section: Discussionsupporting

confidence: 92%

Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes

Fan

Huang

et al. 2013

Cell Cycle

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Section: Discussionsupporting

confidence: 92%

Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes

Fan

Huang

et al. 2013

Cell Cycle

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“…Female fertility is known to decline with advancing age in many species, including mice and humans (11), and this has been partly attributed to increased aneuploidy in oocytes (12). Preliminary studies showed that the incidence of aneuploidy in oocytes of WT females increased from 1.4 to 4.5% between 1 (n ϭ 72 oocytes) and 8 (n ϭ 67 oocytes) mo of age, although this difference was not statistically different (P Ͼ 0.05) by 2 analysis.…”

Section: Resultsmentioning

confidence: 99%

Absence of the proapoptotic Bax protein extends fertility and alleviates age-related health complications in female mice

Perez

Jurisicova

Wise

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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The menopausal transition in human females, which is driven by a loss of cyclic ovarian function, occurs around age 50 and is thought to underlie the emergence of an array of health problems in aging women. Although mice do not undergo a true menopause, female mice exhibit ovarian failure long before death because of chronological age and subsequently develop many of the same age-associated health complications observed in postmenopausal women. Here we show in mice that inactivation of the proapoptotic Bax gene, which sustains ovarian lifespan into advanced age, extends fertile potential and minimizes many age-related health problems, including bone and muscle loss, excess fat deposition, alopecia, cataracts, deafness, increased anxiety, and selective attention deficit. Further, ovariectomy studies show that the health benefits gained by aged females from Bax deficiency reflect a complex interplay between ovary-dependent and -independent pathways. Importantly, and contrary to popular belief, prolongation of ovarian function into advanced age by Bax deficiency did not lead to an increase in tumor incidence. Thus, the development of methods for postponing ovarian failure at menopause may represent an attractive option for improving the quality of life in aging females.aging ͉ apoptosis ͉ menopause ͉ ovary A lthough the process of aging has been attributed in part to increased apoptosis in various tissues (1), animal models lacking cell death-regulatory genes are rarely subjected to longitudinal aging studies. In females, one of the first organ systems to fail with age is the reproductive axis, which in humans is a principal contributing factor to the onset of menopause (2). Ovarian failure, whether natural (age-related) or induced as a consequence of pathological insults, is driven by depletion of ovarian follicles, the hormonally active support structures that house oocytes, through apoptosis (3). As a consequence, dramatic changes in the endocrine activity of the female gonads ensue, which are thought to underlie the emergence of a spectrum of physiological and psychological health complications in aging females (2). Although it has been shown that aged female mice transplanted with ovaries of young donors live longer than nontransplanted control or ovariectomized females (4), it remains unclear whether postmenopausal health complications arise as a direct result of ovarian failure or simply reflect the aging process.Despite the fact that mice do not undergo a true menopause, female mice exhaust their follicle pool long before death because of chronological age (5), similar to that seen in humans (6). Further, aging female mice exhibit many of the same health complications associated with postmenopausal life in women (see below). In a previous study, we reported that oocyte and follicle loss in female mice lacking the proapoptotic Bax protein is attenuated, leading to a dramatic prolongation of ovarian function into very advanced age (7). To better understand the consequences of Bax deficiency and sustained ovar...

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“…It is possible that the same mechanism was involved in the production of other such zygotes with unbalanced haplotypes. The further development of these zygotes would depend on the number and nature of chromosomes escaping regular division The majority, as seen in in-vivo and m-vitro conditions, would be eliminated through natural selection, since their chromosome constitution would not be compatible with survival (Plachot et al, 1988;Pellestor et al, 1994). …”

Section: Micro-manipulation Procedures and Their Possible Genetic Conmentioning

confidence: 99%

Fertilization and early embryology: The chromosomal complements of multipronuclear human zygotes resulting from intracytoplasmic sperm injection

et al. 1996

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Implementation of intracytoplasmic sperm injection (ICSI)in human in-vitro fertilization (IVF) has highlighted the need for information about the risk of nuclear spindle damage caused by this procedure. For this purpose we studied the final products of oocyte meiosis at the first cleavage division of multipronuclear zygotes arising after ICSI, and compared the results with abnormally fertilized oocytes after conventional in-vitro insemination. Of 37 successfully analysed tripronuclear zygotes, 18 had three individual metaphases. Abnormal complements of 11 zygotes in this group indicated that non-disjunction occurred predominantly at the second meiotic division of the oocytes. Nine of the 37 tripronuclear zygotes exhibited two individual metaphases. Seven were abnormal and there were some indications that non-disjunction took place during oocyte meiosis. Of the 37 tripronuclear zygotes, 10 had a single metaphase and three showed an aneuploid number of chromosomes. The overall rate of aneuploidy among tripronuclear microinjected zygotes was 56.7%. In addition, seven zygotes with more than three pronuclei arising after ICSI displayed severely depleted chromosome complements. The incidence of non-disjunction in oocytes fertilized by conventional in-vitro insemination was significantly lower (20.0%, P < 0.01), since only four zygotes had an aneuploid number of chromosomes. Our findings suggest that ICSI might interfere with regular chromosome segregation at the second meiotic division of the oocytes.

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Relationship between morphology and chromosomal constitution in human preimplantation embryo

Cited by 78 publications

References 22 publications

Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes

Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes

Absence of the proapoptotic Bax protein extends fertility and alleviates age-related health complications in female mice

Fertilization and early embryology: The chromosomal complements of multipronuclear human zygotes resulting from intracytoplasmic sperm injection

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