Relationship between muscle fatty infiltration and the biological characteristics and stimulation potential of tenocytes from rotator cuff tears

Klatte‐Schulz, Franka; Gerhardt, Christian; Scheibel, Markus; Wildemann, Britt; Pauly, Stephan

doi:10.1002/jor.22481

Cited by 13 publications

(15 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the cellular or molecular mechanisms behind this relationship are still elusive. We previously showed that TLCs isolated from donors differing in age, sex and MFI exhibit different cellular characteristics and stimulation potential with BMP-2 and BMP-7 [ 18 , 19 , 20 ]. Since MMPs and TIMPs represent the main regulators of modeling and remodeling in the tendon, it was the aim to assess their expression and secretion in TLCs and their supernatant, related to the age, sex and degenerative status of the tendon.…”

Section: Resultsmentioning

confidence: 99%

“…It might be possible that the presence of immune cells such as macrophages or lymphocytes could have affected the results of the study, because they are also able to express MMPs and TIMPs. However, as we could show previously, the tenocyte cultures isolated with the same method were characterized by flow cytometry analysis and expressed hematopoietic markers (CD11b, CD14, CD19, CD34, CD45) in less than 2% [ 18 , 19 , 20 ]. Therefore, the presence of immune cells in the tenocyte cultures can be nearly ruled out.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor Characteristics?

Klatte‐Schulz

Aleyt

Pauly

et al. 2015

IJMS

Self Cite

View full text Add to dashboard Cite

An imbalance between matrix metalloproteases (MMPs) and the tissue inhibitors of metalloproteases (TIMPs) may have a negative impact on the healing of rotator cuff tears. The aim of the project was to assess a possible relationship between clinical and radiographic characteristics of patients such as the age, sex, as well as the degenerative status of the tendon and the MMPs and TIMPs in their tenocyte-like cells (TLCs). TLCs were isolated from ruptured supraspinatus tendons and quantitative Real-Time PCR and ELISA was performed to analyze the expression and secretion of MMPs and TIMPs. In the present study, MMPs, mostly gelatinases and collagenases such as MMP-2, -9 and -13 showed an increased expression and protein secretion in TLCs of donors with higher age or degenerative status of the tendon. Furthermore, the expression and secretion of TIMP-1, -2 and -3 was enhanced with age, muscle fatty infiltration and tear size. The interaction between MMPs and TIMPs is a complex process, since TIMPs are not only inhibitors, but also activators of MMPs. This study shows that MMPs and TIMPs might play an important role in degenerative tendon pathologies.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor Characteristics?

Klatte‐Schulz

Aleyt

Pauly

et al. 2015

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fat infiltration and ossification are pathological signs of tendon degeneration [46]. The differentiation into non-tenocytes is known to induce degenerative processes, which can later manifest as lipid depositions and calcified tissue.…”

Section: Discussionmentioning

confidence: 99%

In Vivo and In Vitro Mechanical Loading of Mouse Achilles Tendons and Tenocytes—A Pilot Study

Fleischhacker

Klatte‐Schulz

Minkwitz

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Mechanical force is a key factor for the maintenance, adaptation, and function of tendons. Investigating the impact of mechanical loading in tenocytes and tendons might provide important information on in vivo tendon mechanobiology. Therefore, the study aimed at understanding if an in vitro loading set up of tenocytes leads to similar regulations of cell shape and gene expression, as loading of the Achilles tendon in an in vivo mouse model. In vivo: The left tibiae of mice (n = 12) were subject to axial cyclic compressive loading for 3 weeks, and the Achilles tendons were harvested. The right tibiae served as the internal non-loaded control. In vitro: tenocytes were isolated from mice Achilles tendons and were loaded for 4 h or 5 days (n = 6 per group) based on the in vivo protocol. Histology showed significant differences in the cell shape between in vivo and in vitro loading. On the molecular level, quantitative real-time PCR revealed significant differences in the gene expression of collagen type I and III and of the matrix metalloproteinases (MMP). Tendon-associated markers showed a similar expression profile. This study showed that the gene expression of tendon markers was similar, whereas significant changes in the expression of extracellular matrix (ECM) related genes were detected between in vivo and in vitro loading. This first pilot study is important for understanding to which extent in vitro stimulation set-ups of tenocytes can mimic in vivo characteristics.

show abstract

“…The majority of studies to date have attempted to modify disease progression or improve healing by adjusting the expression of the fibrotic mediators that was found to be remarkable in diseased tendons, without a comprehensive understanding of their role and mechanisms in the development of the disease. Cells from clinical samples of tendinopathy show an altered phenotype [45] and the cellular activities of RC tear tendon cells may likewise differ according to the severity of the disease [46]. Despite these differential cellular responses, the majority of in vitro tendinopathy studies have not taken this into account.…”

Section: Discussionmentioning

confidence: 99%

Profibrotic mediators in tendon disease: a systematic review

et al. 2016

View full text Add to dashboard Cite

BackgroundTendon disease is characterized by the development of fibrosis. Transforming growth factor beta (TGF-β), bone morphogenic proteins (BMPs) and connective tissue growth factor (CTGF) are key mediators in the pathogenesis of fibrotic disorders. The aim of this systematic review was to investigate the evidence for the expression of TGF-β, BMPs and CTGF along tendon disease progression and the response of tendon cells to these growth factors accordingly.MethodWe conducted a systematic screen of the scientific literature using the Medline database. The search terms used were “tendon AND TGF-β,” “tendon AND BMP” or “tendon AND CTGF.” Studies of human samples, animal tendon injury and overuse models were included.ResultsThirty-three studies were included. In eight studies the expression of TGF-β, BMPs or CTGF was dysregulated in chronic tendinopathy and tendon tear patient tissues in comparison with healthy control tissues. The expression of TGF-β, BMPs and CTGF was increased and showed temporal changes in expression in tendon tissues from animal injury or overuse models compared with the healthy control (23 studies), but the pattern of upregulation was inconsistent between growth factors and also the type of animal model. No study investigated the differences in the effect of TGF-β, BMPs or CTGF treatment between patient-derived cells from healthy and diseased tendon tissues. Tendon cells derived from animal models of tendon injury showed increased expression of extracellular matrix protein genes and increased cell signaling response to TGF-β and BMP treatments compared with the control cells (two studies).ConclusionThe expression of TGF-β, BMPs and CTGF in tendon tissues is altered temporally during healing in animal models of tendon injury or overuse, but the transition during the development of human tendon disease is currently unknown. Findings from this systematic review suggest a potential and compelling role for TGF-β, BMPs and CTGF in tendon disease; however, there is a paucity of studies analyzing their expression and stimulated cellular response in well-phenotyped human samples. Future work should investigate the dynamic expression of these fibrotic growth factors and their interaction with tendon cells using patient samples at different stages of human tendon disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s13075-016-1165-0) contains supplementary material, which is available to authorized users.

show abstract

Relationship between muscle fatty infiltration and the biological characteristics and stimulation potential of tenocytes from rotator cuff tears

Cited by 13 publications

References 42 publications

Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor Characteristics?

Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor Characteristics?

In Vivo and In Vitro Mechanical Loading of Mouse Achilles Tendons and Tenocytes—A Pilot Study

Profibrotic mediators in tendon disease: a systematic review

Contact Info

Product

Resources

About