Relationship between oxidative stress and pathological findings in abomasum of infected lambs by Haemonchus contortus

Machado, Vanessa S.; Silva, Aleksandro S. Da; Schafer, Andressa S.; Aires, Adelina Rodrigues; Tonin, Alexandre Alberto; Oliveira, Camila Belmonte; Hermes, Carine L; Almeida, Taís Corrêa; Moresco, Rafael Noal; Stefani, Lenita M.; Lopes, Sonia T.A.; Mendes, Ricardo E.; Schwertz, Claiton I.; Leal, Marta L.R.

doi:10.1016/j.prp.2014.09.006

Cited by 22 publications

(21 citation statements)

References 29 publications

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“…The infection also did not decrease body weight gain in lambs [17]. It has been recognized that the nematode infection induces the production of reactive oxygen, causing oxidative stress in the hosts [67,68]. The concentration of TBARS in meat in the present study showed a constant increase during storage, which indicated that secondary products of lipid oxidation were accumulated during storage.…”

Section: Plos Onesupporting

confidence: 40%

Ruminal fermentation, microbial population and lipid metabolism in gastrointestinal nematode-infected lambs fed a diet supplemented with herbal mixtures

et al. 2020

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The aim of this study was to evaluate the effects of medicinal herbal mixtures rich in phenolic, flavonoid and alkaloid compounds on ruminal fermentation and microbial populations, and fatty acid (FA) concentrations and lipid oxidation in tissues of lambs infected with the gastrointestinal nematode (GIN) parasite (Haemonchus contortus). Parallel in vitro and in vivo studies were performed using two different herbal mixtures (Mix1 and Mix2). The in vitro study was conducted in a 2 (infection status; non-infected versus infected) × 3 (diets; control, Mix1 and Mix2) factorial design. In the in vivo study, 24 lambs were equally divided into four treatments: non-infected lambs fed a control diet, infected lambs fed the control diet, infected lambs fed a diet with Mix1 and infected lambs fed a diet with Mix2. Herbal mixtures (100 g dry matter (DM)/d) were added to the basal diets of meadow hay (ad libitum) and a commercial concentrate (500 g DM/d). The experimental period lasted for 70 days. Ruminal fermentation characteristics and methane production were not affected by infection in vivo or in vitro. Both herbal mixture supplementation increased total volatile fatty acid (VFA) concentrations (P < 0.01) and DM digestibility (P < 0.01) in vitro. Archaea population was slightly diminished by both herbal mixtures (P < 0.05), but they did not lower methane production in vitro or in vivo (P > 0.05). Infection of H. contortus or herbal mixtures modulated FA proportion mainly in the liver, especially the long chain FA proportion. Concentrations of thiobarbituric acid reactive substances (TBARS) in serum were significantly higher after 70 days post-infection in the infected lambs. Herbal Mix1 supplementation reduced TBARS concentrations in meat after seven days of storage. In conclusion, supplementing of herbal mixtures to the diets of GIN parasite infected lambs did not affect the basic ruminal fermentation parameters. Herbal mixtures may improve few FA proportions mainly in liver as well as decrease lipid oxidation in meat.

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Section: Plos Onesupporting

confidence: 40%

Ruminal fermentation, microbial population and lipid metabolism in gastrointestinal nematode-infected lambs fed a diet supplemented with herbal mixtures

et al. 2020

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“…Pivoto et al [58] determined the total oxidation status and antioxidant capacity of lambs infected with H. contortus and reported increased oxidative stress in lambs' sera. On the other hand, increase in levels of Ferric Reducing Ability of Plasma (FRAP), an indicator of TAC in sera of lambs, was observed during infection with H. contortus [59]. The authors suggested that TAC increased to minimize the effect of reactive oxygen species (ROS) produced during infection with H. contortus.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effect of Balanites Aegyptiaca Fruit’s Ethanol Extract in Egyptian Baladi Goats Experimentally Infected with Haemonchus Contortus: Blood Serum Biochemical, Oxidative Stress Markers and Pathological Studies

et al. 2020

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the native breed of goats that is located in the Nile Delta and along the Nile Valley is known as Baladi goats. Amongst other breeds of goats, the Baladi goats have a great economic value [1] and play an essential role in the economy of small, marginal farmers and landless labourers in Egypt [2]. T HE THERAPEUTIC effect of Balanites aegyptiaca fruit's ethanolic extract (BAF-EE) in goats experimentally infected with Haemonchus contortus (H. contortus) was evaluated. Serum biochemical parameters, tissue oxidative stress status and pathological changes were investigated. Twelve goats were divided into 4 equal groups, G1 (infected-untreated), G2 (infected-BAF-EE treated), G3 (infected-albendazole treated) and G4 (uninfected-BAF-EE treated). Infection was conducted at 0-time, whereas treatment with BAF-EE and albendazole was given in the 5 th week post infection (PI). Blood samples were drawn on 0-time, 3 rd week PI and then biweekly till the 9 th week for serum biochemical analyses. At the end of experiment, all animals were slaughtered to estimate oxidative stress markers and histopathological alterations in the abomasal and hepatic tissues. Serum biochemistry of infected animals showed significant depression of total serum proteins, albumin, albumin/ globulin ratio, total antioxidant capacity and calcium levels. The activity of AST, ALT and γGT and the cholesterol, urea and creatinine levels were significantly elevated in the serum of infected animals. Glutathione peroxidase values decreased significantly in tissue homogenate of G1 and G3 goats than those in G2 and G4 goats. Malondialdehyde followed a reverse course. Abomasa of infected animals in G1 revealed inflammation, necrosis, petechial hemorrhages and cellular infiltration. Liver showed bile duct hyperplasia, degeneration of hepatocytes and cell infiltration. Intensity of alterations were lower in infected-treated animals. In conclusion, BAF-EE has hepatoprotective effect and can improve serum biochemical alterations and oxidative stress status in goats infected with H. contortus, justifying its use as a safe natural anthelmintic agent.

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“…The presence of nematodes in sheep abomasa is usually associated with the production of reactive oxygen species by immune cells, which can represent host defensive mechanisms against parasites but can also generate oxidative stress (34). Oxidative stress arises as an imbalance between the formation of oxidants and antioxidants in the body and can damage lipids, DNA, proteins, and carbohydrates, which eventually lead to tissue damage and in ammation (25). Traditional medicinal plants have long been known for their antioxidant properties.…”

Section: Discussionmentioning

confidence: 99%

“…Mucosal mast-cell hyperplasia, the formation of globular leukocytes, eosinophilia (an increase in eosinophils in blood and tissue), increased mucus production in the abomasum, and the production of speci c antibodies typically emerge during infection (22)(23)(24). The presence of nematodes in the abomasum can also induce the production of reactive oxygen species by immune cells, which can damage the parasites and cause oxidative stress in the host (25). Plant secondary metabolites may improve the health of animals through their antioxidant and immune-enhancing properties (26,27).…”

Section: Introductionmentioning

confidence: 99%

Effect of Artemisia Absinthium and Malva Sylvestris on Antioxidant Parameters and Abomasal Histopathology in Lambs Experimentally Infected with Haemonchus Contortus

Mravčáková

Sobczak-Filipiak

Váradyová

et al. 2020

Preprint

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Background: Haemonchus contortus is a blood-feeding gastrointestinal nematode (GIN) that parasitizes in the abomasum of ruminants. Infections caused by this nematode possess a serious threat to livestock production worldwide. The influence of medicinal plants using for control of haemonchosis on the local immune response of abomasal mucosae in GIN-infected sheep, however, has not been reported. This study evaluated the effect of Artemisia absinthium and Malva sylvestris as dietary supplements containing bioactive compounds on antioxidant response and histopathological changes in the abomasa of the lambs. Twenty-four lambs were divided into four groups: unsupplemented lambs (UNS), lambs supplemented with A. absinthium (ART), lambs supplemented with M. sylvestris (MAL), and lambs supplemented with both plants (ARTMAL). All lambs were infected orally with approximately 5000 L3 larvae of H. contortus. The experiment was conducted for 75 d, all animals were then slaughtered, and the abomasal tissues were examined for antioxidant parameters and histopathology.Results: Methanolic extracts of wormwood and mallow were separated into four fractions by semi-preparative high-performance liquid chromatography, which identified saccharides, phenols, phenolic acids, flavonoids, polyphenols, esters, fatty acids, lipids, and other substances. The concentration of malondialdehyde in the abomasal mucosa was lower in ARTMAL (P < 0.05), and the total antioxidant capacity was higher in MAL (P < 0.05), than in UNS. Serum antioxidant parameters were not affected by the treatments (P > 0.05). Histopathological changes were mainly detected in the mucosal membrane of the abomasum, with the presence of infiltrates of inflammatory cells in MAL, ART, and ARTMAL. Increase mucus production was observed in the ARTMAL. The number of mast cells was significantly higher in UNS and ART than MAL (P < 0.01 and P < 0.05). Plasma cell numbers were higher in ARTMAL than MAL (P < 0.05). Abomasal tissue regenerated more frequently in ARTMAL than the other experimental groups.Conclusions: These results represent the first report of the impact of A. absinthium and M. sylvestris on antioxidant parameters and local immune responses of abomasal mucosa of lambs infected with a GIN parasite. Wormwood and mallow applied as dietary supplements may increase the resistance of lambs to GIN infections.

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Relationship between oxidative stress and pathological findings in abomasum of infected lambs by Haemonchus contortus

Cited by 22 publications

References 29 publications

Ruminal fermentation, microbial population and lipid metabolism in gastrointestinal nematode-infected lambs fed a diet supplemented with herbal mixtures

Ruminal fermentation, microbial population and lipid metabolism in gastrointestinal nematode-infected lambs fed a diet supplemented with herbal mixtures

Therapeutic Effect of Balanites Aegyptiaca Fruit’s Ethanol Extract in Egyptian Baladi Goats Experimentally Infected with Haemonchus Contortus: Blood Serum Biochemical, Oxidative Stress Markers and Pathological Studies

Effect of Artemisia Absinthium and Malva Sylvestris on Antioxidant Parameters and Abomasal Histopathology in Lambs Experimentally Infected with Haemonchus Contortus

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