Relationship between physical activity and markers of oxidative stress in independent community-living elderly individuals

Fraile-Bermúdez, Ana Belén; Kortajarena, Maider; Zarrazquin, Idoia; Maquibar, Amaia; Yanguas, José Javier; Sánchez-Fernández, Cristina; Gil, Javier; Irazusta, Amaia; Ruiz‐Litago, Fátima

doi:10.1016/j.exger.2015.07.005

Cited by 23 publications

(17 citation statements)

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“…Exercise has generally been found to increase antioxidant enzyme capacity while decreasing lipid peroxidation and DNA damage markers in healthy populations of men and women. [39][40][41][42][43][44][45][46][47][48][49][50][51] However, not all studies showed consistency across biomarkers, 40 42 45 46 52 and some studies have shown no effect of exercise on oxidative stress or antioxidant capacity. 53 54 In these findings, study designs and populations are heterogeneous.…”

Section: Discussionmentioning

confidence: 99%

Effects of exercise on markers of oxidative stress: an Ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention Trial

Friedenreich

Pialoux

Wang

et al. 2016

BMJ Open Sport Exerc Med

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BackgroundOxidative stress may contribute to cancer aetiology through several mechanisms involving damage to DNA, proteins and lipids leading to genetic mutations and genomic instability. The objective of this study was to determine the effects of aerobic exercise on markers of oxidative damage and antioxidant enzymes in postmenopausal women.MethodsThe Alberta Physical Activity and Breast Cancer Prevention Trial (ALPHA) was a two-centre, two-armed randomised trial of 320 inactive, healthy, postmenopausal women aged 50–74 years. Participants were randomly assigned to a year-long exercise intervention (225 min/week) or a control group while being asked to maintain a normal diet. Fasting blood samples were obtained and plasma concentrations of two oxidative damage markers (8-hydroxy-2′-deoxyguanosine (8-OHdG) and 8-isoprostaglandin F2α (8-Iso-PGF2α)) and two antioxidant enzymes (superoxide dismutase and catalase) were measured at baseline, 6 months and 12 months. Intention-to-treat (ITT) and per-protocol analyses were performed using linear mixed models adjusted for baseline biomarker concentrations. A further exercise adherence analysis, based on mean minutes of exercise per week, was also performed.ResultsIn the ITT and per-protocol analyses, the exercise intervention did not have any statistically significant effect on either oxidative damage biomarkers or antioxidant enzyme activity.ConclusionsA year-long aerobic exercise intervention did not have a significant impact on oxidative stress in healthy, postmenopausal women.Trial registration numberNCT00522262.

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Section: Discussionmentioning

confidence: 99%

Effects of exercise on markers of oxidative stress: an Ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention Trial

Friedenreich

Pialoux

Wang

et al. 2016

BMJ Open Sport Exerc Med

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“…MDA is an important indicator, which reflects the extent of the attack by free radical and its content can reflect the degree of lipid peroxidation in the cell (Sehirli et al 2008). Fraile-Bermudez et al (2015) found that the GPx antioxidant emzyme activity was positively correlated with CAT activity and that the antioxidant enzymes SOD and GPx negatively correlated with MDA (Mladenov et al 2015). The current experiment mainly determined the MDA and ROS contents and a degree of antioxidant enzyme activity, and our results showed that the contents of MDA and ROS in the VA-treated group were significantly lower than in the control, indicating that VA enhanced cellular antioxidant function, which is consistent with the results of previous studies.…”

Section: Discussionmentioning

confidence: 99%

Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide

Shi¹,

Yan²,

Jin³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The considerable increase in oxygen requirements due to the high metabolic rate of the bovine mammary epithelial cells (BMEC) during lactation results in an augmented production of reactive nitrogen species (RNS), such as nitric oxide (NO), which may expose cows to increased oxidative stress. Vitamin A (VA) has been shown in several studies to enhance the antioxidant defence system against oxidative stress, but whether the reason is related to a reduced NO production remains unclear. Diethylene triamine-nitric oxide polymer (NOp) is a type of NO-generating compound, which is safe, efficacious, and releases NO over a long period. The current study was conducted to investigate the effect of VA on the antioxidant function in BMEC and the underlying mechanism by discussing the protection of VA on NO-induced oxidative stress of BMEC. The experiment was conducted using a single-factor completely randomized arrangement. Primary BMEC were isolated from the mammary glands of Holstein dairy cows. The third generation cells were randomly divided into four equal groups with six replicates each. Each group received different combinations of VA and NOp treatment as follows: controls (without VA and NOp), NOp treatment alone, VA treatment alone, and VA and NOp treatment together. The lysates were collected to evaluate the activities of glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) and the contents of reactive oxygen species (ROS) and malondialdehyde (MDA), and the cell-free supernatants were collected to analyze selenoprotein P (SelP) content, inducible nitric oxide synthase (iNOS) activities and nitric oxide (NO), interleukin-1 (IL-1), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) contents. The results suggested that compared to the control, the cell proliferation, the activity of the antioxidants GPx and TrxR, the content of SelP and the antioxidant gene expressions of GPx1, GPx4, and TrxR1 were significantly decreased (P < 0.05), and the contents of ROS and MDA, the activity of iNOS, the contents of NO and IL-1, IL-6, TNF-α, and their mRNA expressions were increased dramatically in the NOp treatment alone group (P < 0.05), but the opposite changes were observed in the VA treatment alone group. Compared to the NOp treatment alone, the VA and NOp treatment together significantly improved cell proliferation, the activities of the antioxidants GPx and TrxR, and the gene expressions of GPx1 and TrxR1, and dramatically decreased the contents of ROS and MDA, the activity of iNOS, the contents of NO and IL-1, IL-6, TNF-α and their mRNA expression levels (P < 0.05). The present research suggests that VA can improve the antioxidant function of BMEC and protect the cells from experiencing the NOp-induced oxidative stress by regulating antioxidant gene expression. The probable mechanism is that VA can reduce the activity of iNOS and its mRNA expression by down-regulating of the expression of IL-1, IL-6, and TNF-α to reduce NO production. However, the exact mechanism warrants future exploration. Keyword...

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“…Regular PA at moderate intensity may induce favourable changes in antioxidant defence system, especially by an increase of individual enzymatic antioxidants activity (124 were positively correlated with CAT activity (116). The MVPA and cpm were negatively correlated with lipid peroxidation whereas PA was positively correlated with CAT activity in males (116).…”

Section: Discussionmentioning

confidence: 96%

“…Physical activity (PA) increases generation of Reactive Oxygen Species (ROS), which have a high oxidising capacity (116). However, ROS are important signalling molecules involved in crucial biological process at suitable levels such as muscle concentration, which are essential mechanisms to avoid cell damage after PA.…”

Section: Introductionmentioning

confidence: 99%

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The impact of physical activity and fitness on health biomarkers and oxidative stress in adults aged over 55 years = Impacto de la actividad y la condición física sobre biomarcadores de salud y estrés oxidativo en adultos mayores de 55 años

Ugarriza¹

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Que la Tesis Doctoral titulada "The impact of physical activity and fitness on health biomarkers and oxidative stress in adults aged over 55 years. Impacto de la actividad y la condición física sobre biomarcadores de salud y estrés oxidativo en adultos mayores de 55 años" que presenta Dña. RAQUEL APARICICO UGARRIZA al superior juicio del Tribunal que designe la Universidad Politécnica de Madrid, ha sido realizada bajo mi dirección durante los años 2013-2017, siendo expresión de la capacidad técnica e interpretativa de su autora en condiciones tan aventajadas que le hacen merecedor del Título de Doctor con mención Internacional, siempre y cuando así lo considere el citado Tribunal. Que la Tesis Doctoral titulada "The impact of physical activity and fitness on health biomarkers and oxidative stress in adults aged over 55 years. Impacto de la actividad y la condición física sobre biomarcadores de salud y estrés oxidativo en adultos mayores de 55 años"que presenta Dña. RAQUEL APARICICO UGARRIZA al superior juicio del Tribunal que designe la Universidad Politécnica de Madrid, ha sido realizada bajo mi dirección durante los años 2013-2017, siendo expresión de la capacidad técnica e interpretativa de su autora en condiciones tan aventajadas que le hacen merecedor del Título de Doctor con mención Internacional, siempre y cuando así lo considere el citado Tribunal.

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Relationship between physical activity and markers of oxidative stress in independent community-living elderly individuals

Cited by 23 publications

References 45 publications

Effects of exercise on markers of oxidative stress: an Ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention Trial

Effects of exercise on markers of oxidative stress: an Ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention Trial

Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide

The impact of physical activity and fitness on health biomarkers and oxidative stress in adults aged over 55 years = Impacto de la actividad y la condición física sobre biomarcadores de salud y estrés oxidativo en adultos mayores de 55 años

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