Relationship between physicochemical properties and enzymatic hydrolysis of sugarcane bagasse varieties for bioethanol production

Brienzo, Michel; Tyhoda, Luvuyo; Benjamin, Yuda; Görgens, Johann F.

doi:10.1016/j.nbt.2014.12.007

Cited by 48 publications

(41 citation statements)

References 35 publications

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“…These results indicated that the efficiency of enzymatic hydrolysis was significantly affected by the remaining lignin content, and it increased as the lignin content decreased. In general, cellulose conversion is known to decrease as the CrI of cellulose increases, and it has been already reported that the CrI is related to cellulose content or alfa-cellulose [31]. However, this phenomenon was not observed in the data of Samples 1–3 or 5 in this study.…”

Section: Resultscontrasting

confidence: 71%

“…The region between 55 and 110 ppm was dominated by strong signals assigned mostly to various cellulosic carbons, and the sharp signal at 104.7 ppm is attributed to the ordered cellulose C-1. The signals at 68–80 ppm are due to cellulose C-2, C-3, and C-5 [30, 31]. The relative intensity of the signal at 56.0 ppm, which corresponds to the methoxyl groups (–OCH 3 ) of aromatic moieties in lignin, decreased as the NaOH concentration increased until nearly disappearing entirely in the spectrum of Sample 5.…”

Section: Resultsmentioning

confidence: 99%

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Valorization of lignin and cellulose in acid-steam-exploded corn stover by a moderate alkaline ethanol post-treatment based on an integrated biorefinery concept

Yang

Zhang

Wen

et al. 2016

Biotechnol Biofuels

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BackgroundDue to the unsustainable consumption of fossil resources, great efforts have been made to convert lignocellulose into bioethanol and commodity organic compounds through biological methods. The conversion of cellulose is impeded by the compactness of plant cell wall matrix and crystalline structure of the native cellulose. Therefore, appropriate pretreatment and even post-treatment are indispensable to overcome this problem. Additionally, an adequate utilization of coproduct lignin will be important for improving the economic viability of modern biorefinery industries.ResultsThe effectiveness of moderate alkaline ethanol post-treatment on the bioconversion efficiency of cellulose in the acid-steam-exploded corn stover was investigated in this study. Results showed that an increase of the alcoholic sodium hydroxide (NaOH) concentration from 0.05 to 4% led to a decrease in the lignin content in the post-treated samples from 32.8 to 10.7%, while the cellulose digestibility consequently increased. The cellulose conversion of the 4% alcoholic NaOH integrally treated corn stover reached up to 99.3% after 72 h, which was significantly higher than that of the acid steam exploded corn stover without post-treatment (57.3%). In addition to the decrease in lignin content, an expansion of cellulose I lattice induced by the 4% alcoholic NaOH post-treatment played a significant role in promoting the enzymatic hydrolysis of corn stover. More importantly, the lignin fraction (AL) released during the 4% alcoholic NaOH post-treatment and the lignin-rich residue (EHR) remained after the enzymatic hydrolysis of the 4% alcoholic NaOH post-treated acid-steam-exploded corn stover were employed to synthesize lignin-phenol-formaldehyde (LPF) resins. The plywoods prepared with the resins exhibit satisfactory performances.ConclusionsAn alkaline ethanol system with an appropriate NaOH concentration could improve the removal of lignin and modification of the crystalline structure of cellulose in acid-steam-exploded corn stover, and consequently significantly improve the conversion of cellulose through enzymatic hydrolysis for biofuel production. The lignin fractions obtained as byproducts could be applied in high performance LPF resin preparation. The proposed model for the integral valorization of corn stover in this study is worth of popularization.

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Section: Resultscontrasting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Valorization of lignin and cellulose in acid-steam-exploded corn stover by a moderate alkaline ethanol post-treatment based on an integrated biorefinery concept

Yang

Zhang

Wen

et al. 2016

Biotechnol Biofuels

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show abstract

“…The same variation in glucose yield was also observed among the varieties when a high cellulase enzyme load was applied in the enzymatic hydrolysis (15 FPU/g cellulase enzyme supplemented with 15 U/g β ‐glycosidase). After 24, 48, and 72 h of reaction, the varieties that presented lower content of lignin also presented higher yields of glucose …”

Section: Lignin Effects On Enzymatic Hydrolysismentioning

confidence: 99%

“…It has the sole aim of reducing its recalcitrance. During this step, the glycosidic bonds linking the individual sugar molecular chains are broken down, resulting in changes in the physical and chemical structure of the cell walls of the lignocellulosic material . Further, during this step the pretreatment chemicals penetrate the biomass and solubilize lignin from the secondary walls, making it possible for the enzymes to access and hydrolyze the polysaccharides.…”

Section: Pretreatments Effects On Biomass Componentsmentioning

confidence: 99%

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Sugarcane biomass conversion influenced by lignin

Schmatz

Tyhoda

Brienzo

2019

Biofuels Bioprod Bioref

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Plant biomass residues are renewable sources for the production of biofuels and high‐value macromolecules. Sugarcane bagasse is one such plant biomass residue that is available from the sugar‐processing industry. It is used as a raw material for biobased ethanol production. However, some of its properties and its behavior during processing have a major inhibitory effect on its successful conversion. Chief among these inhibitory properties are the lignin content, its distribution in plant tissues, and its chemical properties. These make the materials naturally resistant to bioconversion processes. Further, lignin and carbohydrate degradation products can be formed during acid pretreatment, which is one of the major steps during biomass conversion to bioethanol. These products negatively affect the liberation of fermentable sugars and the yield of ethanol during the fermentation stage of the conversion process. Other factors that also have an influence on the production of fermentable sugar are related to the different structural arrangement of plant tissues (cane fractions of the node, internode, and external fraction), as well as biomass variety. Biomass varieties with low lignin content result in an improved yield of fermentable sugars, which in turn contributes to improved viability of the second‐generation bioethanol production processes. By selecting sugarcane varieties with the best properties, ethanol production can be increased without increasing the total area under cultivation. Efforts have been dedicated to reducing biomass recalcitrance by classical and precision breeding. Genetic modification of sugarcane alters the genes responsible for the encoding enzymes for lignin biosynthesis, generating sugarcane with low recalcitrance. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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Sieving process selects sugarcane bagasse with lower recalcitrance to xylan solubilization

Alves

Melati

Casagrande

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUNDSugarcane bagasse is a heterogeneous and organized material that needs a pretreatment to access the polysaccharides (cellulose and xylan). The particle size of a non‐milled bagasse can influence the xylan and xylose solubilization, as well as the enzymatic hydrolysis of the pretreated material. Using bagasse selected on 16‐, 30‐, 40‐ and 50‐mesh sieves: xylan solubilization was performed with hydrogen peroxide; xylose was solubilized with diluted sulfuric acid; and enzymatic hydrolysis was conducted for glucose yield evaluation.RESULTSThe xylose solubilized by acid pretreatment resulted in a maximum of 6.59 g L−1 with no influence of particle size. The solubilized xylan showed linear correlation with a variation of 36.18% (16‐mesh bagasse) to 71.43% (<50‐mesh bagasse). Pretreated material submitted to enzymatic hydrolysis revealed that particle size influenced the pretreatment, increasing the glucose yield.CONCLUSIONSThe results showed that non‐milled sugarcane bagasse particle size selection positively influenced the solubilization of xylan, improving the enzymatic hydrolysis of the pretreated material. © 2020 Society of Chemical Industry (SCI)

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Relationship between physicochemical properties and enzymatic hydrolysis of sugarcane bagasse varieties for bioethanol production

Cited by 48 publications

References 35 publications

Valorization of lignin and cellulose in acid-steam-exploded corn stover by a moderate alkaline ethanol post-treatment based on an integrated biorefinery concept

Valorization of lignin and cellulose in acid-steam-exploded corn stover by a moderate alkaline ethanol post-treatment based on an integrated biorefinery concept

Sugarcane biomass conversion influenced by lignin

Sieving process selects sugarcane bagasse with lower recalcitrance to xylan solubilization

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