Relationship between respiratory burst and adhesiveness capacity in elderly polymorphonuclear cells

Tortorella, Cosimo; Ottolenghi, A; Pugliese, Piervito; Jirillo, Emilio; Antonaci, Salvatore

doi:10.1016/0047-6374(93)90071-x

Cited by 37 publications

(15 citation statements)

References 30 publications

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“…The aging-related decrease in CD69 expression is not compensated for by an increase in the number of circulating PMN in elderly subjects [34]. Thus this impairment in the externalization of CD69-containing vesicles is likely to be related to the impairments in PMN phagocytosis, bactericidal activity and release of reactive oxygen species [7] seen with increasing age. Such changes contribute to the ageassociated decrease in cellular immunity, which may predispose older people to infectious disease and tumours [35][36][37].…”

Section: Pmn Heterogeneitymentioning

confidence: 85%

“…These processes are reduced in PMN from elderly subjects [7]. In order to determine whether aging is associated with alterations in vesicle fusion, we examined the effects of aging on the process of insertion of two antigens into the plasma membrane of human PMN following cell activation in vitro : CD11b, a β2 integrin essential for PMN adhesion, chemotaxis and phagocytosis [8] ; and CD69, an early activation marker whose role in PMN and T-cell activation has yet to be fully elucidated [9].…”

Section: Introductionmentioning

confidence: 96%

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Effect of aging on CD11b and CD69 surface expression by vesicular insertion in human polymorphonuclear leucocytes

1999

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The exocytosis of intracellular vesicles is an important function of the plasma membrane, which is responsible for hormone secretion, cell surface expression of antigens, ion transporters and receptors, and intracellular and intercellular signalling. Human aging is associated with many physiological and cellular changes, many of which are due to alterations in plasma membrane functioning. Alterations in vesicle externalization with age could account for many of these changes. We investigated whether alterations in vesicle exocytosis occur with increasing age by flow-cytometric determination of CD11b and CD69 expression on the surface of human polymorphonuclear leucocytes (PMN) stimulated with phorbol myristate acetate (PMA), a tumour promoter which binds to and activates protein kinase C (PKC) directly, or with formyl-Met-Leu-Phe (fMLP), which activates PKC indirectly via interactions with a cell surface receptor and G-protein, and subsequent inositol phosphate hydrolysis. Following stimulation with PMA, a decrease in the proportion of PMN expressing CD69 at high levels was observed in elderly compared with young subjects (young, 55.3%; elderly, 43.9%; P=0.01). No aging-related differences in the proportion of PMN expressing CD11b (young, 73.7%; elderly, 68.4%; P=0.15), or in the number of molecules of CD69 or CD11b expressed per cell, were observed. Stimulation with fMLP or low PMA concentrations resulted in full CD11b expression but minimal CD69 expression in both young and elderly subjects. Cells which expressed CD69 had no CD11b expression, while those cells expressing CD11b had minimal CD69 expression. Thus the PMA-induced expression of CD11b and CD69 in human PMN represents two separate processes, only one of which is affected in aging. CD11b expression appears to require a lesser degree of PKC stimulation compared with that required for CD69 expression. The age-associated reduction in PMA-stimulated CD69 expression may occur either at or distal to PKC activation. Such a decrease may contribute to the age-associated impairments in PMN function that contribute, in turn, to immunosenescence.

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Section: Pmn Heterogeneitymentioning

confidence: 85%

Section: Introductionmentioning

confidence: 96%

Effect of aging on CD11b and CD69 surface expression by vesicular insertion in human polymorphonuclear leucocytes

1999

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“…The authors concluded that the decreased phagocytic index in the older subjects was secondary to an age-related decline in surface CD16 expression, necessary for Fc-mediated phagocytosis. (25) However, other groups have reported a diminished respiratory burst and protection from apoptosis from PMNs collected in older healthy subjects upon engagement of the surface TREM-1 receptor (26) or fMLP (27) , but a similar production of reactive oxygen species (ROS) with PMA stimulation. (28) Thus, the capacity to produce ROS may be intact, but specific signaling pathways to evoke ROS production may be deficient in the elderly.…”

Section: Age-related Changes In Innate Immunitymentioning

confidence: 99%

“…(25) However, other groups have reported a diminished respiratory burst and protection from apoptosis from PMNs collected in older healthy subjects upon engagement of the surface TREM-1 receptor (26) or fMLP (27) , but a similar production of reactive oxygen species (ROS) with PMA stimulation. (28) Thus, the capacity to produce ROS may be intact, but specific signaling pathways to evoke ROS production may be deficient in the elderly. In addition, it has been observed that GM-CSF, IL-2 or LPS treatment of neutrophils from the elderly were less protected from apoptosis than neutrophils from younger subjects.…”

Section: Age-related Changes In Innate Immunitymentioning

confidence: 99%

Age-related changes in immune function: Effect on airway inflammation

Busse¹,

Mathur²

2010

Journal of Allergy and Clinical Immunology

162

141

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Immunosenescence is defined as changes in the innate and adaptive immune response associated with increased age. The clinical consequences of immunosenescence include increased susceptibility to infection, malignancy, and autoimmunity, decreased response to vaccination and impaired wound healing. However, there are several immune alterations which may facilitate persistence of asthma into late adulthood, or development of asthma after the age of 50-60 years. Asthma in older patients is not uncommon and is a growing population as the average lifespan increases. Specific innate changes which may affect severity of asthma in older patients, or be involved in the development of late onset asthma include impaired in mucociliary clearance, and changes in airway neutrophil, eosinophil and mast cell numbers and function. Additionally, age-related altered antigen presentation and decreased in specific antibody responses may increases the risk of respiratory infections. Respiratory infections exacerbate asthma in older patients, and possibly play a role in the pathogenesis of late onset asthma. Furthermore, cytokine profiles may be modified with aging; some investigators suggesting a trend towards Th2 cytokine expression. This review examines specific innate and adaptive immune responses affected by aging that may impact the inflammatory response in older adults with asthma.

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“…However, the ability of neutrophils to kill phagocytosed organisms is diminished in the elderly compared to younger individuals[42], which may be due to a decrease in the production of reactive oxygen species (ROS)[43;44]. In addition, it has been observed that neutrophils in the elderly are more prone to undergoing apoptosis, which may be due to a deficiency in the cytokine-mediated signaling pathways to protect the neutrophils[45;46].…”

Section: Immunosensecencementioning

confidence: 99%

Changes in Immune Function in Asthma in the Elderly

Mathur

Nyenhuis

2009

Aging Health

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Asthma is an inflammatory disorder of the airway. The airway inflammation of asthma is typically an allergic inflammation characterized by cells and mediators described as a “Th2” inflammatory response. There is a growing body of evidence describing changes in the function of immune cells upon aging, a phenomenon referred to as “immunosenescence”. Several studies utilizing animal models and human subjects with asthma have begun to explore age-related effects on the airway inflammation in asthma. This review explores the existing data on the presence and effects of immunosenescence or age-related changes in immune function in asthma.

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Relationship between respiratory burst and adhesiveness capacity in elderly polymorphonuclear cells

Cited by 37 publications

References 30 publications

Effect of aging on CD11b and CD69 surface expression by vesicular insertion in human polymorphonuclear leucocytes

Effect of aging on CD11b and CD69 surface expression by vesicular insertion in human polymorphonuclear leucocytes

Age-related changes in immune function: Effect on airway inflammation

Changes in Immune Function in Asthma in the Elderly

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