Relationship between serum anti-heat shock protein 27 antibody levels and obesity

Kargari, Mehrdad; Tavassoli, Samira; Avan, Amir; Ebrahimi, Mahmoud; Azarpazhooh, Mahmoud Reza; Asoodeh, Rasool; Nematy, Mohsen; Hassanian, Seyed Mahdi; Rahmani, Farzad; Mohammadzade, Elham; Esmaeili, Habibollah; Moohebati, Mohsen; Ferns, Gordon A.; Parizadeh, Seyed Mohammad Reza

doi:10.1016/j.clinbiochem.2017.02.015

Cited by 11 publications

(16 citation statements)

References 26 publications

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“…Sahebkar et al showed an increase in anti-HSP27 antibody levels in patients with coronary heart disease (CHD) and MetS compared to individuals with CHD alone (Sahebkar et al 2011), which is in agreement with the study of Ghayour-Mobarhan et al (25). Kargari et al found that obese individuals showed significantly higher anti-HSP27 antibody levels than the non-obese group (26). However, they did not find any association between anti-HSP27 antibody titers and either diabetes or MetS.…”

Section: Discussionsupporting

confidence: 63%

Is there any association between Serum anti-HSP27 antibody level and the presence of metabolic syndrome; population based case-control study

Sadabadi

Heidari‐Bakavoli

Esmaily

et al. 2019

Revista Romana De Medicina De Laborator

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Background: Heat shock protein 27 (HSP27) is an intracellular chaperone constitutively expressed in many cell types including cardio myocytes and endothelial cells. Circulating levels of HSP27 and anti-HSP27 antibody are higher in patients with CVD. Anti-HSP27 antibody concentrations were also reported to be increased in atherogenesis. We aimed to evaluate serum anti-HSP27 antibody titers in individuals with, or without, MetS in the MASHAD study cohort with large sample size in 6,568 subjects. Methods: Participants with MetS were identified from MASHAD cohort (n=3358) using the IDF criteria, and the control group were those individuals who did not meet these criteria (n=3210). In-house enzyme-linked immune sorbent assay (ELISA) method was used for measuring Anti-HSP27 antibody levels. The two groups were matched for age, sex and smoking habit. Results: As expected, there were significant differences in height (p= 0.004), waist and hip circumference, weight, BMI, systolic and diastolic blood pressure, TGs, TC, HDL-C, Hs-CRP, glucose, with the presence of diabetes mellitus, hypertension, hyperlipidemia (p<0.001) between the two groups. Serum HSP27 antibody titers did not show significant difference between the groups with and without metabolic syndrome (p= 0.740). Conclusion: In conclusion, our results revealed serum anti-HSP27 antibody titers were not statistically different between individuals with and without MetS. However, it is possible that drug treatment may affect antibody titers and confound our findings in this population sample..

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Section: Discussionsupporting

confidence: 63%

Is there any association between Serum anti-HSP27 antibody level and the presence of metabolic syndrome; population based case-control study

Sadabadi

Heidari‐Bakavoli

Esmaily

et al. 2019

Revista Romana De Medicina De Laborator

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“…HSPB1 (also known as HSP27) serves as an ATP-independent chaperone [ 30 ]. In mammals, it has been reported that obese subjects had higher anti-HSP27 antibody levels [ 31 ], and induction of HSP27 may blunt the adverse effect of fat overexposure on insulin function [ 32 ]. In diabetic hearts, the phosphorylation of HSP27 enhanced lipoprotein lipase activity and promoted hydrolysis of triglyceride-rich lipoproteins to fatty acids [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Wei

Gong

et al. 2018

BMC Genomics

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BackgroundIn avian species, liver is the main site of de novo lipogenesis, and hepatic lipid metabolism relates closely to adipose fat deposition. Using our fat and lean chicken lines of striking differences in abdominal fat content, post-hatch lipid metabolism in both liver and adipose tissues has been studied extensively. However, whether molecular discrepancy for hepatic lipid metabolism exists in chicken embryos remains obscure.ResultsWe performed transcriptome and proteome profiling on chicken livers at five embryonic stages (E7, E12, E14, E17 and E21) between the fat and lean chicken lines. At each stage, 521, 141, 882, 979 and 169 differentially expressed genes were found by the digital gene expression, respectively, which were significantly enriched in the metabolic, PPAR signaling and fatty acid metabolism pathways. Quantitative proteomics analysis found 20 differentially expressed proteins related to lipid metabolism, PPAR signaling, fat digestion and absorption, and oxidative phosphorylation pathways. Combined analysis showed that genes and proteins related to lipid transport (intestinal fatty acid-binding protein, nucleoside diphosphate kinase, and apolipoprotein A-I), lipid clearance (heat shock protein beta-1) and energy metabolism (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 and succinate dehydrogenase flavoprotein subunit) were significantly differentially expressed between the two lines.ConclusionsFor hepatic lipid metabolism at embryonic stages, molecular differences related to lipid transport, lipid clearance and energy metabolism exist between the fat and lean chicken lines, which might contribute to the striking differences of abdominal fat deposition at post-hatch stages.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4776-9) contains supplementary material, which is available to authorized users.

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“…Ninety-two seronegative healthy individuals, as a part of the large MASHAD Cohort Study [15], were enrolled as subjects without CHB, at the aforementioned time. They were recruited based on age and gender matching and were proven negative for HBV, HCV, and HDV by serology examinations (Diapro HBsAg, HCV Ab, HDV Ab ELISA Kits, Italy).…”

Section: Study Populationmentioning

confidence: 99%

“…The lower limit of HBV DNA quanti cation for this assay was 100 copies/mL. Blood sample collection and laboratory analysis for subjects without CHB, have been conducted with the same protocols as a part of the MASHAD Cohort Study [15], carried out at Mashhad University of Medical Sciences, and the whole related data was transferred to our study.…”

Section: Sample Collection and Laboratory Analysesmentioning

confidence: 99%

“…More importantly, the creation of sHSP27 and anti-HSP27-Ab immune complexes have been reported to enhance the function of sHSP27 by altering the signaling of crucial receptors involved in the in ammatory processes [13]. As antibodies are simply measured by blood tests and are often more stable and easily detectable due to higher concentrations [14], recent studies seek to discover the role of circulating anti-HSP27-Ab in various diseases [9,15,16]. Higher levels of the anti-HSP27-Ab have been recently reported in chronic liver disease patients with a positive history of alcohol consumption [14].…”

Section: Introductionmentioning

confidence: 99%

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Association Between Serum Levels of Anti-heat Shock Protein 27 Antibody and Liver Cell Injury in Chronic Hepatitis B

Yousefli

Meshkat²,

Ghayour‐Mobarhan³

et al. 2023

Ind J Clin Biochem

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BackgroundHeat Shock Protein 27 (HSP27), an anti-HBV factor, exists in the intracellular and extracellular spaces. Serum HSP27 (sHSP27) is an in ammatory modulator and is associated with elevated pro-in ammatory cytokines and with a higher likelihood of hepatocellular carcinoma. SHSP27 results in natural antibody production (anti-HSP27-Ab) that is more stable and easily detectable compared to sHSP27. We aimed to investigate any potential association between anti-HSP27-Ab level and chronic hepatitis B (CHB) progression and in ammation indicated by liver cell injury and HBV replication. MethodsThis cross-sectional study was conducted on 91 patients with CHB and 92 individuals without CHB. Following demographic data collection, anti-HSP27-Ab, serum lipids including total cholesterol, triglyceride, LDL-C, HDL-C, and aminotransferase levels were measured using enzymatic assays in participants' serum samples. HBV DNA was also measured by quantitative PCR in CHB patients. ResultsBivariate and multivariate analyses showed a signi cantly higher mean level of anti-HSP27-Ab in CHB than in healthy individuals (0.304 vs. 0.256AU/ml, P-value = 0.015). These levels held signi cant differences in the CHB subgroups of male patients, at the age of 50 years and above, non-smokers, patients with elevated aminotransferase levels, and hypotriglyceridemia (P-value < 0.05). However, no difference was found between the antibody levels and HBV DNA copies (P-value > 0.05). ConclusionThis study provides evidence that anti-HSP27 antibody levels can re ect the degree of liver necrosis indicated by aminotransferase levels. Regarding the higher incidence rate of HBV-associated complications in 50 to 60-year-old men, monitoring the antibody can be bene cial in managing this group of CHB patients, which deserves further investigation.

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Relationship between serum anti-heat shock protein 27 antibody levels and obesity

Abstract: We have found that serum anti-HSP27 antibody titers are related to several cardiovascular risk factors, necessitating further studies on the value of this emerging marker for risk stratification.

Cited by 11 publications

References 26 publications

Is there any association between Serum anti-HSP27 antibody level and the presence of metabolic syndrome; population based case-control study

Is there any association between Serum anti-HSP27 antibody level and the presence of metabolic syndrome; population based case-control study

Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Association Between Serum Levels of Anti-heat Shock Protein 27 Antibody and Liver Cell Injury in Chronic Hepatitis B

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