Relationship between sperm quality traits and field-fertility of porcine semen

Tsakmakidis, Ιoannis; Lymberopoulos, A. G.; Khalifa, T. A. A.

doi:10.4142/jvs.2010.11.2.151

Cited by 69 publications

(61 citation statements)

References 20 publications

(24 reference statements)

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“…While several studies have used samples from bulls and humans (Januskauskas et al, 2003;Bungum et al, 2004;Lucio et al, 2016), few have correlated sperm morphology and chromatin instability in swine (Saravia et al, 2007;Tsakmakidis et al, 2010). Further, many studies that correlated morphological data with chromatin instability did not distinguish normal cells from those with cytoplasmic droplets, therefore, it is not possible to determine whether the presence of cytoplasmic droplets is related to chromatin instability.…”

Section: Introductionmentioning

confidence: 95%

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

Gaggini¹,

Rocha²,

Souza³

et al. 2017

Anim Reprod

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In boar studs, morphological analyses are used to evaluate sperm quality and thereby categorize ejaculates as either approved or rejected. Normally, morphological characteristics correlate with chromatin disorders, but studies to date have only considered the average of abnormalities; cells were not segregated as normal or abnormal. The aim of this study was to assess whether the presence of cytoplasmic droplets was associated with morphometric characteristics and chromatin instability of spermatozoa heads. Morphological analyses were performed on semen from 11 boars using phase contrast microscopy (200 cells per sample). Normal cells were differentiated from those with cytoplasmic droplets and both types were evaluated separately. Photomicrographs were acquired of normal spermatozoa (Group NOR, N = 1,207) as well as spermatozoa with proximal and distal cytoplasmic droplets (Group DROP, N = 725). Sperm-head morphometry and chromatin structure were evaluated using the toluidine blue technique. Spermatozoa heads in the DROP group were longer (8.37 ± 0.60 × 8.31 ± 0.53; P = 0.025), narrower (4.16 ± 0.21 × 4.19 ± 0.19; P = 0.03), and more symmetric on the sides (0.973 ± 0.012 × 0.971 ± 0.011; P = 0.007) than were spermatozoa heads of the NOR group. The DROP group also had a greater average ellipticity (0.335 ± 0.034 × 0.329 ± 0.031; P = 0.0004), a greater percentage of decondensed chromatin (2.71 ± 3.87 × 2.28 ± 1.38; P < 0.0008), and a greater chromatin heterogeneity (4.66 ± 1.40 × 4.40 ± 1.42; P < 0.0001). A greater frequency of semen collection results in a shorter period of cell maturation and this probably affected the degree of chromatin condensation and the cytoplasmic droplet migration, with concomitant effect on the head morphometry measurements. In conclusion, compared with normal spermatozoa, those with cytoplasmic droplets show altered morphometric characteristics, such as longer and narrower spermatozoa heads. They likewise have greater chromatin instability, resulting in a higher percentage of decondensed chromatin and more heterogeneous chromatin.

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Section: Introductionmentioning

confidence: 95%

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

Gaggini¹,

Rocha²,

Souza³

et al. 2017

Anim Reprod

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show abstract

“…Although the relationship between sperm head dimensions and chromatin status remains controversial, many of the studies reported no relationship between them (Saravia et al, 2007;Gassuto et al, 2011). Aiming at improving the predictive value of semen analysis, earlier investigations supported a relation between fi eld fertility and chromatin integrity after the combination of sperm traits such as live normal spermatozoa and stability of the deoxyribonucleic acid complex (BoeHansen et al, 2008;Tsakmakidis et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Age-related changes in quality and fertility of porcine semen

Tsakmakidis

Khalifa²,

Boscos

2012

Biol. Res.

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The aim of this study was to investigate the eff ect of boar age on quality traits and fertility of liquid-stored semen. Boars were allocated into 3 age groups: 7-10 months (young), 18-33 months (mature), 51-61 months (old). Ejaculates of ≥ 200x10 6 sperm/ml and 85% total motile sperm were extended to 30x10 6 sperm/ml, stored at 17-18 °C and used within 12-24 h for artifi cial insemination (AI) of 2062 multiparous sows. After 24 h of storage, aliquots of diluted semen were assessed for sperm progressive motility (SPM), incidence of sperm chromatin instability (SCI), proportion of live morphologically normal sperm (LMNS) and head morphometry of LMNS. The results showed that young boars had higher percentages of SCI and lower proportions of LMNS than those of the mature (p < 0.05) and old (p < 0.001) boars, respectively. Sperm head dimensions of young and old boars were greater (p < 0.03-0.001) than those of mature boars. The farrowing rate of young boars (65%) was signifi cantly lower (p < 0.001; χ 2 = 30-61) than those of the mature (87.2%) and old (84.7%) boars. The relationship between sperm head dimensions and boar fertility was non-signifi cant. In conclusion, boar age is an important physiological factor contributing to the success of swine AI.

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“…However, the high farrowing rate may not always be relative to high litter size. Moreover, weak correlations between farrowing rate and litter size were found in other studies, indicating that these traits may be affected differently by the semen quality of the boars (Tsakmakidis et al, 2010;Juonala et al, 1998).…”

Section: Dead Sperm Live Spermmentioning

confidence: 95%

“…After this assessment, two sperm populations were identified: live green-stained sperm (SYBR-14 + /PI -); and dead red-stained sperm (SYBR-14 -/PI + ). Sperm morphology was determined microscopically after staining the semen samples with eosin nigrosin stain on a slide (Tsakmakidis et al, 2010). Briefly, 7µL of boar semen was added to 20µL eosin nigrosin staining solution in a 0.6 mL micro-centrifuge graduated tube and mixed gently.…”

mentioning

confidence: 99%

Relationship between sperm plasma membrane integrity and morphology and fertility following artificial insemination

Matabane¹,

Thomas²,

Netshirovha³

et al. 2017

SA J. An. Sci.

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Sperm quality plays an important role in determining fertility. The aim of the study was to examine the relationship between sperm plasma membrane integrity and morphology, and fertility following artificial insemination (AI). A total of 16 ejaculates were collected from three Large White boars using the gloved hand technique. The semen was extended with a commercial extender. The AI dose contained 80 mL semen sample (3 × 10 9 sperm/mL). Aliquots of diluted semen were assessed for sperm plasma membrane integrity (synthetic binding CD-14 (SYBR + )/propidium iodide (PI -) and sperm morphology (eosin nigrosin). A total of 73 Duroc-type, Large White and nondescript multiparous sows from smallholder farms were inseminated with extended semen samples. Boar sperm plasma membrane integrity and morphology were subjected to one-way analysis of variance (ANOVA). The average boar sperm plasma membrane integrity and normal sperm morphology were 78.6% and 77.2%, respectively. The average conception and farrowing rates following artificial insemination (AI) were 78.1 and 57.5%, respectively. A negative correlation was observed between sperm plasma membrane integrity and fertility. There was a weak positive correlation between normal sperm morphology and conception rate (r = 0.11). Additionally, a relationship was observed between normal sperm morphology and litter size (r = 0.37) and total number born alive (r = 0.03), although relatively low. In conclusion, a negative relationship was found between sperm plasma membrane integrity and fertility. Moreover, there was a relationship between morphologically normal sperm and litter size, as well as number of piglets born alive, although relatively low. ___________________________________________________________________________________ Keywords: Boar, Eosin Nigrosin, Semen quality, SYBR14/PI # Corresponding author: NedambaleTL@tut.ac.zaImproving the qualitative and quantitative analysis of boar semen samples to estimate fertility potential of males is critical for a successful breeding programme. Although much progress has been made, the ability to predict the fertility of semen with traditional laboratory tests is still limited, owing mainly to the complexity of sperm morphological damage and their fertilization potential (Brito et al., 2003). In addition, the prediction of sperm fertilizing ability is of great economic importance to breeding of sows as it leads to the selection of boars with better semen fertility, which results in good reproductive performance. Semen fertility trait assessments play a crucial role in the early detection of developmental disorders in male animals (Smital et al., 2004). As well as traditional methods of semen assessment, biochemical tests and morphological analyses of sperm are performed. Disturbances in spermatogenesis give rise to morphological sperm defects (Kavak et al., 2004). Moreover, recent studies have indicated a correlation between normal morphological

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Relationship between sperm quality traits and field-fertility of porcine semen

Cited by 69 publications

References 20 publications

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

Age-related changes in quality and fertility of porcine semen

Relationship between sperm plasma membrane integrity and morphology and fertility following artificial insemination

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