Relationship between spermatozoon movement velocity and expression of testicular isoform of angiotensin-converting enzyme on their surface

Aleksinskaya, Marina A.; Nikolaeva, M. A.; Danilov, Sergei M.; Kuz'michyov, L. N.; Levchuk, T. N.; Yelistratova, O. S.; Сухих, Г. Т.

doi:10.1007/s10517-006-0137-9

Cited by 3 publications

(4 citation statements)

References 12 publications

(15 reference statements)

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“…The amount of membrane bound tACE, measured by ELISA also correlate negatively with sperm motility [ 15 ]. The levels of tACE measured by flow cytometry correlate in opposite manners with sperm motility depending on the sperm samples used [ 16 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Localization and Distribution of Testicular Angiotensin I Converting Enzyme (ACE) in Neck and Mid-Piece of Spermatozoa from Infertile Men in Relation to Sperm Motility

Pencheva

Keskinova

Rashev

et al. 2021

Cells

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Testicular angiotensin converting enzyme (ACE) is known to play an essential role in the male reproduction and fertility. Data about tACE in cases of male infertility are quite scarce, and in this respect we aimed to study localization and distribution of tACE protein in the neck and mid-piece of spermatozoa from pathological samples in relation to sperm motility. The enzyme expression during capacitation and acrosome reaction was quantitatively assessed. In human ejaculated spermatozoa tACE is localized on sperm plasma membrane of the head, the neck and mid-piece of the tail. The immunoreactivity becomes stronger in capacitated spermatozoa followed by a decrease in acrosome reacted sperm. In different cases of semen pathology (oligozoospermia, asthenozoospermia and teratozoospermia) fluorescent signals in the neck and mid-piece are in punctate manner whereas in normozoospermia they were uniformly distributed. The expression area of tACE the neck and mid-piece was decreased in ejaculated and capacitated sperm from pathological semen samples compared to normospermia. Significant positive correlation was established between tACE area and progressive sperm motility, whereas with immotile sperm the correlation was negative. Our data suggest that proper distribution of tACE in the neck and mid-piece is required for normal sperm motility that could be used as a novel biomarker for male infertility.

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Section: Discussionmentioning

confidence: 99%

Localization and Distribution of Testicular Angiotensin I Converting Enzyme (ACE) in Neck and Mid-Piece of Spermatozoa from Infertile Men in Relation to Sperm Motility

Pencheva

Keskinova

Rashev

et al. 2021

Cells

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“…The highest concentrations of tACE have been reported on spermatozoa with normal morphological characreristics and motility [13]. Moreover, positive correlation exists between spermatozoon movement and expression of tACE [14]. Thus, tACE expression on human spermatozoa is of clinical importance for the diagnosis of some forms of male infertility.…”

Section: Discussionmentioning

confidence: 99%

Expression of testicular angiotensin-converting enzyme in adult spontaneously hypertensive rats.

Atanassova

Lakova²,

Bratchkova³

et al. 2009

Folia Histochem Cytobiol.

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Recent studies demonstrated that one isoform of angiotensin-converting enzyme named testicular or germinal (tACE) is localized in postmeiotic male germ cells and is essential for fertilizing ability of spermatozoa. Hypertension in spontaneously hypertensive rats (SHR) is androgen-dependent and reduction in male gametes is reported in this experimental conditions. Expression of tACE was not studied under conditions of spontaneous hypertension. The aim of this work is to characterize immunoexpression of tACE in the testis of adult (16-week-old) SHR rats in relation to the changes in blood pressure and serum testosterone level. In 82% of adult SHR, the immunoexpression of tACE followed the normal stage-specific pattern. Destructive testicular changes, germ cells depletion have been observed in 18% of 16-week-old SHR and stronger expression of tACE in stages 8-11compared to controls was detected. As a result stage specificity in SHR was not as evident as in control. No reaction was found in germ cell depleted tubules in which elongated spermatids were absent. Degenerating germ cells exhibited strong immunostaining comparable to that in residual bodies. The blood pressure was significantly higher in SHR and testosterone levels were more than twice but non-significantly elevated. There was no clear correlation between testicular structural changes, blood pressure level values or serum testosterone levels. Expression of tACE in postmeiotic germ cells, specifically altered by SHR, suggested possible involvement of components of reninangiotensin system in the process of spermiogenesis. Loss of enzyme expression we found in germ cell depleted tubules in SHR is due to absence of corresponding stages of spermatid differentiartion. Therefore, tACE can be used as a marker for germ cell depletion due to hypertension and other pathological conditions.

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“…The highest tACE concentration has been reported in spermatozoa with normal morphological features and motility [47] . The RAM, used as an ACE inhibitor herein, provided a partial beneficial contribution without preventing the depression of sperm parameters caused by the DXR.…”

Section: Akman öZkanlar öZkanlar Oruç Ulaş Zi̇ypak Lehi̇mci̇oğlu Tmentioning

confidence: 95%

“…However, there has been no effect on the sperm motility in longterm Captopril-treated humans [18] . On the other hand, the influence of ACE on the receptors localised on the sperm neck and tail to bind A-II may affect the sperm thrusting movement [47] , while the underlying mechanism of its effect on the sperm motility remains unclear. Herein, although some beneficial effects were observed with the DP treatment, its usage alone against the DXR-toxicity could not prevent the deterioration in sperm motility, live-dead sperm rate, density and head abnormalities, but it markedly prevented the increase in the total abnormal sperm rate.…”

Section: Akman öZkanlar öZkanlar Oruç Ulaş Zi̇ypak Lehi̇mci̇oğlu Tmentioning

confidence: 99%

Eritropoietin Hormonu ve ACE İnhibitörü, Erişkin Erkek Sıçanların Sperm Parametrelerini Doxorubicin Toksisitesine Karşı Korur

AKMAN¹,

ORUÇ²,

LEHİMCİOĞLU³

et al. 2015

Kafkas Univ Vet Fak Derg

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Doxorubicin (DXR) is used against the cancer but it has some adverse effects (gonadotoxicity, cardiotoxicity and nephrotoxicity). We aimed to determine the effect of DXR toxicity on reproduction and whether Darbepoetin (DP) and Ramipril (RAM) treatments play a protective role against this toxicity in male rats. Herein, adult male Sprague-Dawley rats (n=34) were divided randomly into five groups, as control (Group I; n=7, no medication) and four treatment groups (II to V). DXR was administered to all treatment groups (DXR, 2.5 mg/kg/w i.v. for 3 weeks) and they were Group II (DXR, n=6), Group III (n=7, DP 10 µg/kg/w i.p. for three weeks), Group IV (n=7, RAM 1 mg/kg/d p.o. for four weeks), and Group V (n=7, DP+RAM). Rats in all groups were sacrificed after four weeks of treatment. Spermatological parameters along with histopathological images and malondialdehyde levels of testicular tissue were evaluated. Weights of body, testicles, cauda epididymis and male accessory glands in DXR-treated groups were significantly different (P<0.05) from the control group. DXR toxicity adversely altered all the sperm parameters. But, DP plus RAM treatment in Group V improved the DXR-depressed motility and viable sperm rate. Sperm concentrations significantly decreased (P<0.05) in DXR-treated groups as compared to control group. In Group III and V, the increase in total abnormal sperm rate caused by the DXR injections was prevented. In conclusion, this study indicated that weekly DXR injections in adult rats depressed all the epididymal sperm parameters. Co-treatment by DP and RAM protected the sperm cells against the toxicity due to the DXR administration while single usages of the DP or RAM provided partial improvements. Keywords: Doxorubicin toxication, Erythropoietin, ACE inhibitor, Rat sperm Eritropoietin Hormonu ve ACE İnhibitörü, Erişkin Erkek Sıçanların Sperm Parametrelerini Doxorubicin Toksisitesine Karşı Korur ÖzetKanser tedavisinde kullanılan Doxorubicin'in (DXR), kardiyotoksik ve nefrotoksik yan etkileri ile birlikte gonadotoksik özelliği de bulunmaktadır. Bu çalışmada, yetişkin erkek Sprague-Dawley sıçanlardaki sperm parametreleri üzerine DXR'nin toksik etkisi ile Darbepoetin (DP) ve Ramipril (RAM) tedavisinin bu toksikasyonu önleyici bir rolünün olup olmadığı araştırıldı. Yetişkin 34 adet erkek Sprague-Dawley sıçanlar rastgele 5 gruba ayrıldı. Kontrol grubunda bulunan sıçanlarda (Grup I; n=7) hiçbir ilaç kullanılmadı. Tüm tedavi gruplarında bulunan sıçanlara DXR uygulaması (DXR, 2.5 mg/kg/hafta i.v. 3 hafta, n=27) yapıldı ve bu gruplar Grup II (DXR, n=6), Grup III (DP 10 µg/kg/hafta i.p. 3 hafta, n=7), Grup IV (RAM 1 mg/kg/gün p.o. 4 hafta, n=7) ve Grup V'ten (DP+RAM, n=7) oluştu. Dört haftalık deneysel çalışma sonrasında tüm sıçanlara ötenazi uygulandı. Spermatolojik parametrelerle birlikte histopatolojik muayene ve testiküler dokulardaki malondialdehit (MDA) düzeyleri incelendi. DXR uygulanan gruplardaki ölçülen vücut, testis, kauda epididimis ve erkek eklenti bezlerinin ağırlıkları ile kontrol grubu parame...

show abstract

Relationship between spermatozoon movement velocity and expression of testicular isoform of angiotensin-converting enzyme on their surface

Cited by 3 publications

References 12 publications

Localization and Distribution of Testicular Angiotensin I Converting Enzyme (ACE) in Neck and Mid-Piece of Spermatozoa from Infertile Men in Relation to Sperm Motility

Localization and Distribution of Testicular Angiotensin I Converting Enzyme (ACE) in Neck and Mid-Piece of Spermatozoa from Infertile Men in Relation to Sperm Motility

Expression of testicular angiotensin-converting enzyme in adult spontaneously hypertensive rats.

Eritropoietin Hormonu ve ACE İnhibitörü, Erişkin Erkek Sıçanların Sperm Parametrelerini Doxorubicin Toksisitesine Karşı Korur

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