Relationship between stationary and mobile phase composition and its influence on retention factors of aromatic hydrocarbons in reversed-phase high-performance liquid chromatography

Nasuto, R.; Kwietniewski, L.; Różyło, J. K.

doi:10.1016/s0021-9673(96)00859-x

Cited by 11 publications

(6 citation statements)

References 11 publications

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“…In the first interval (30-40%), log k versus methanol content decreased slower than in the range of the content varied from 40 to 50%. This phenomenon contradicted previous works concerning the separation of aromatic hydrocarbons [45,46]. Theoretically, log k versus methanol content plots are directly related to the adsorption isotherm of methanol that increases as the content of methanol in mobile phase decreases.…”

Section: Separation Of Barbituratescontrasting

confidence: 80%

Investigation of the novel mixed-mode stationary phase for capillary electrochromatography

Ohyama

Shirasawa

Wada

et al. 2004

Journal of Chromatography A

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Section: Separation Of Barbituratescontrasting

confidence: 80%

Investigation of the novel mixed-mode stationary phase for capillary electrochromatography

Ohyama

Shirasawa

Wada

et al. 2004

Journal of Chromatography A

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“…In the presence of ODS silica gel particles in these mixed solvents, the excessive adsorption of solvent molecules onto the alkyl chain of ODS results in a change in solute adsorption capacity. 25,26 Owing to their low solubility in n-alkane, ACN and MeOH solvate on the surface of the ODS alkyl chain, forming an interfacial liquid phase that is more concentrated than the bulk liquid phase. 27−30 Steinhoff et al used molecular dynamics simulations to demonstrate the formation of an interfacial liquid phase on the ODS alkyl chain with higher concentrations of ACN and MeOH.…”

Section: ■ Introductionmentioning

confidence: 99%

Effect of Organic Solvent on the Mass Transfer Mechanism of Coumarin 102 in a Single Octadecylsilyl Silica Gel/Organic Solvent–Water System by Laser Trapping and Fluorescence Microspectroscopy

Yamada,

Miyagawa,

Honma

et al. 2024

Langmuir

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Understanding mass transfer kinetics within individual porous particles is crucial for theoretically explaining the retention and elution behaviors in chromatography and drug delivery. Using laser trapping and fluorescence microspectroscopy, we investigated the diffusion mechanism of coumarin 102 (C102) into single octadecylsilyl particle in acetonitrile (ACN)/water, N,N-dimethylformamide (DMF)/water, and 1-butanol (BuOH)/ water solutions. The intraparticle diffusion behavior of C102 was evaluated using the spherical diffusion equation, allowing us to determine the intraparticle diffusion coefficients (D intra ): (8−10) × 10 −9 cm 2 s −1 for ACN, (10−16) × 10 −9 cm 2 s −1 for DMF, and (4− 6) × 10 −9 cm 2 s −1 for BuOH. The obtained D intra values were further analyzed using a pore and surface diffusion model. Thus, we revealed that the diffusion mechanism of C102 differed depending on the organic solvent: surface diffusion for ACN and DMF and pore and surface diffusions for BuOH were observed. This difference is attributed to the formation of a concentrated liquid phase of ACN and DMF at the interface of the alkyl chain and the bulk solution in the pore.

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“…It is widely accepted that retention could be caused by adsorption of the analytes onto the adsorbent surface [28] and/or partitioning of the analytes between the stationary and the mobile phases [29,30]. The properties of the stationary phase strongly depend on the qualitative and quantitative compositions of the mobile phase [31]. The components of the mobile phase may adsorb on the stationary phase, forming a layer or even many layers [32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Retention and Separation Selectivity of Aromatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Stationary Phases and Eluents

2020

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In this manuscript, the retention of aromatic hydrocarbons with polar groups has been compared for systems with various nonpolar columns of the types from C3 to C18 and different mobile phases composed of methanol, acetonitrile, or tetrahydrofuran as modifiers. The selectivity separation of the solutes in systems with different adsorbents, when one eluent modifier is swapped by another, has been explained, taking into account molecular interactions of the solutes with components of the stationary phase region (i.e., extracted modifier depending on the chain length of the stationary phase).

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Relationship between stationary and mobile phase composition and its influence on retention factors of aromatic hydrocarbons in reversed-phase high-performance liquid chromatography

Cited by 11 publications

References 11 publications

Investigation of the novel mixed-mode stationary phase for capillary electrochromatography

Investigation of the novel mixed-mode stationary phase for capillary electrochromatography

Effect of Organic Solvent on the Mass Transfer Mechanism of Coumarin 102 in a Single Octadecylsilyl Silica Gel/Organic Solvent–Water System by Laser Trapping and Fluorescence Microspectroscopy

Comparison of the Retention and Separation Selectivity of Aromatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Stationary Phases and Eluents

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