Relationship between the initial rate of protein aggregation and the lag period for amorphous aggregation

Kinetics of protein fibril formation: Methods and mechanisms

“…Also, the relation between the initial aggregation time and the protein concentration is defined by a simple equation [60]:…”

“…The value of n=1 would suggest that the rate-limiting step is protein unfolding whereas n>1 would suggest that aggregation of protein to form nucleus is the rate limiting step [60]. This simple model has been extended to understand the disease mechanism of fibril formation as well [65].…”

Quantification of anti-aggregation activity of UV-irradiated α-crystallin

“…Reduction of α- The time-course of the increase in the light scattering intensity (I) accompanying aggregation of α-lactalbumin was analyzed using the approaches elaborated by us previously [39,60]. To calculate the lag period (t 0 ) on the dependences of I on time and to characterize the initial rate of aggregation, we applied the following empiric equation:…”

“…Our previous studies [39,60] have shown that DTT-induced aggregation of α-lactalbumin proceeds through a stage of formation of the start aggregates, and t 0 is a moment of time at which the start aggregates emerge.…”

“…6B; v 0 is the v value measured in the absence of α-crystallin). The order of aggregation with respect to the protein (n) was taken equal to 5.3 [60]. From the initial part of the dependence of (v/v 0 ) 1/n on x the initial adsorption capacity of α-crystallin (AC 0 ) with respect to α-lactalbumin was calculated: AC 0 = 1.18 ± 0.07 molecules of α-lactalbumin per one subunit of α-crystallin.…”