Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay

Larson-Cook, Kjersten L.; Brannian, John D.; Hansen, Keith A.; Kasperson, K.M.; Aamold, Edward T; Evenson, Donald P.

doi:10.1016/s0015-0282(03)01116-6

Cited by 341 publications

(214 citation statements)

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“…Sperm DNA fragmentation is increasingly being recognized as an important cause of infertility. Clinical studies indicate that DNA fragmentation levels above 30% measured by the SCSA, are not compatible with the initiation and maintenance of a term pregnancy (2).…”

Section: Introductionmentioning

confidence: 94%

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Relationship Between Standard Semen Parameters, Calcium, Cholesterol Contents, and Mitochondrial Activity in Ejaculated Spermatozoa From Fertile and Infertile Males

Meseguer

Garrido

Martínez-Conejero

et al. 2004

J Assist Reprod Genet

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Purpose : To correlate levels of cholesterol (CH), calcium (Ca 2+ ), and mitochondrial activity (MA) with the standard semen parameters and to compare them between fertile and infertile men. Methods : We studied 151 semen samples from infertile (n = 60) or fertile (n = 91) males. Basic sperm parameters were analyzed. Ca 2+ and CH concentrations on seminal plasma were determined by enzymoimmunoanalysis. Intracellular Ca 2+ and CH concentrations in the sperm plasma membrane and mitochondrial activity by fluorometry. Results : There was a significant positive correlation between sperm membrane CH and sperm morphology. Intracellular Ca 2+ was lower in infertile patients compared to fertile. No differences were found regarding Ca 2+ and CH concentrations in seminal plasma. MA is directly and strongly related with sperm motility. Conclusions : Intracellular concentrations of Ca 2+ and the proportion of CH in the sperm membrane are two important markers of the sperm quality due to its direct relationship with sperm morphology and fertility potential.

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Section: Introductionmentioning

confidence: 94%

“…To date we lack data regarding molecular aspects of male infertility, useful to be employed as a diagnostic test, apart from those results obtained by classical WHO (1) analysis and the recent sperm chromatin structure assay (SCSA) (2,3). Sperm DNA fragmentation is increasingly being recognized as an important cause of infertility.…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Standard Semen Parameters, Calcium, Cholesterol Contents, and Mitochondrial Activity in Ejaculated Spermatozoa From Fertile and Infertile Males

Meseguer

Garrido

Martínez-Conejero

et al. 2004

J Assist Reprod Genet

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“…The need to reevaluate old test scores is best illustrated by how a reevaluation of the use of the SCSA DNA fragmentation index (DFI) scores has dramatically changed how the test results are used to direct patients to appropriate ART [16,17]. The SCSA is the only DNA integrity test with substantial scientific literature supporting the credibility of this test for use in diagnosing male infertility, and up until 2007, patients whose sperm had a DNA fragmentation index (DFI)≥30 were told that their sperm had minimal to no chance for successful ART attempts at pregnancy [22,31,32]. However, as the number of clinics using ICSI in treating their infertile couples, this DFI cut-off began to be questioned as many groups using sperm with DFIs of 30 or higher were having excellent live birth outcome rates [23,43,60].…”

Section: Discussionmentioning

confidence: 99%

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

Brown

Merryman²,

Rivnay

et al. 2013

J Assist Reprod Genet

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Purpose The objective of this study was to evaluate a panel of three sperm function tests; tests known to assess different aspects of sperm functionality and genomic integrity, the: 1) Sperm DNA Accelerated Decondensation (SDAD TM ) Test, 2) Sperm DNA Decondensation (SDD TM ) Test, and 3) Sperm Penetration Assay (SPA), determining if positive and negative test scores correlated with failed and successful ICSI outcomes, respectfully. Methods A prospective, double blinded, cohort study was performed. One study sample (ejaculated semen) was collected by each of the 60 male partners of the 60 couples enrolled in the study; males whose female partners were found to have no major female factor issues. The sperm from each male was analyzed in the SPA, and SDAD and SDD Tests, and used for ICSI (1 ICSI cycle per couple). Results The ICSI cycle pregnancy rate for this study was 50 %, with a delivery rate040 % (n060 ICSI cycles). The SPA and SDD Test scores did not significantly predict ICSI outcome when used as stand-alone tests (p>>0.05). However, when the SPA and SDD Test scores were used together, ICSI outcomes for a subgroup of 10 (16.7 %) males, were significantly predicted (p00.03), with 1 live birth, and 9 negatives where the transferred embryos did not implant. In total, 38.4 % of the couples in this study were found to have a very poor chance for a successful ICSI cycle. Conclusion SDAD Test scores alone, and SPA and SDD Test scores used together, significantly predicted failed ICSI outcomes. This indicates that the scores obtained when analyzing patients' sperm using a panel of sperm function tests; specifically, the SPA, and SDAD and SDD Tests, can be used to identify infertile couples who should not be directed to ICSI.

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“…35 However, high levels of sperm DNA damage appear to correlate inversely to successful pregnancy with IUI, IVF, and IVF/ICSI. 36 In the largest retrospective study to date, Bungum et al 37 did not find increased pregnancy loss with high sperm DNA fragmentation rates with IVF/ICSI but did find a ten-fold lower risk of pregnancy with IUI. This study also demonstrated significantly higher clinical pregnancy rates (52.9% vs 22.2%) and delivery rates (47.1% vs 22.2%) with IVF/ICSI compared with IVF in couples with high sperm DNA damage.…”

Section: Sperm Chromatin Dispersionmentioning

confidence: 95%

“…These data confirm earlier observations that IVF outcomes are affected more profoundly than are IVF/ICSI results by DNA damage. 36 Thus, as the literature on this assay accrues, the assessment of sperm DNA integrity may become an important predictor of pregnancy success in the setting of assisted reproduction.…”

Section: Sperm Chromatin Dispersionmentioning

confidence: 99%

An Assessment of New Sperm Tests for Male Infertility

Natali

Turek

2011

Urology

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The routine semen analysis, although used for more than 50 years, fails to accurately distinguish between fertile and infertile men. As a consequence, many tests of sperm function (TSF) have been developed. This review discusses both older and newer diagnostic TSF. It outlines the principles underlying each assay and reviews aggregate clinical data to determine its current relevance and utility. It concludes that the relevance of many older TSF is questionable, with the wide acceptance of intracytoplasmic sperm injection (ICSI). Newer TSF have the potential to deliver more clinically relevant information but require more extensive study to better understand their predictive role in the ICSI era. UROLOGY 77: 1027-1034, 2011. © 2011 Elsevier Inc. All rights reserved.I n addition to the medical history and physical examination, the conventional semen analysis has been an essential laboratory test for the evaluation of male fertility for at least 50 years. However, the idea that fertility can be defined by threshold values of semen parameters is a concept that is fundamentally flawed. 1 Although not a true measure of fertility, the semen analysis, if abnormal, suggests that the probability of achieving fertility is lower than normal. 2 Because of biological variability, two semen analyses are generally needed, performed with two to three days of sexual abstinence and evaluated in a standardized fashion. 2 Recognized normal values for semen parameters are given in Table 1 in agreement with the latest recommendations by the World Health Organization (WHO). 2 Traditionally, (except for the latest WHO recommendations), these references are derived by expert consensus and not by prospective clinical trials and thus their true relationship to male fertility is unclear. In addition, the definition of what constitutes "normal" semen parameters is constantly challenged. A meta-analysis of 29 US studies of semen quality from 9612 fertile, or presumably fertile, men suggested that a sperm concentration of 98 million/mL is normal. 3 Although sperm motility is considered the "best" predictor of fertility, normal sperm motility ranges from 53% to 62%. 4 Thus, simply deriving "normal" semen parameters has been a prohibitively lengthy and inconclusive process to date.Finally, other biological variables affect the clinical utility of the semen analysis. In addition to wide intraindividual variation, seasonal 5 and geographic variation 4 further complicate the performance of the semen analysis as a fertility measure. As examples, within-subject, interejaculate coefficients of variation for sperm concentration and motility are estimated at 44.7% and 15% in one study. 1 Thus, even with excellent quality control, wide biological variation in semen quality profoundly challenges the notion that the conventional semen analysis can accurately assess male fertility. OLDER ADJUNCTIVE SPERM TESTSBecause of the need to more precisely characterize normal fertile semen, adjunctive semen testing has become popular (Figure 1). The concept behind de...

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Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay

Cited by 341 publications

References 36 publications

Relationship Between Standard Semen Parameters, Calcium, Cholesterol Contents, and Mitochondrial Activity in Ejaculated Spermatozoa From Fertile and Infertile Males

Relationship Between Standard Semen Parameters, Calcium, Cholesterol Contents, and Mitochondrial Activity in Ejaculated Spermatozoa From Fertile and Infertile Males

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

An Assessment of New Sperm Tests for Male Infertility

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