Relationship Between the Radiation Dose and Chromosome Aberrations in Atomic Bomb Survivors of Hiroshima and Nagasaki

G-Banding analysis of radiation-induced chromosome damage in lymphocytes of hiroshima A-bomb survivors

“…The dose-response relationship for the frequencies of cells with chromosome aberrations has been demonstrated by Awa et al, (1971Awa et al, ( , 1978 and Preston etal. (1988) in the peripheral blood lymphocytes of A-bomb survivors in Hiroshima and Nagasaki using the conventional cytogenetic method.…”

“…It is noteworthy that a small fraction of cells that lose a portion of chromosomes, defined as "deletions," has been detected even by the non-banded conventional analysis (Awa et al, 1977). However, the conventional cytogenetic method could not discriminate between two types of deletions, terminal and interstitial.…”

Is FISH painting an appropriate biological marker for dose estimates of suspected accidental radiation overexposure? A review of cases investigated in France from 1995 to 1996.

“…A gradual decrease in the frequency of cells carrying Dics generally is reported with increasing time after exposure (4). Some contrary examples were reported; for example, the presence of cells carrying unstable aberrations detected decades postirradiation in atomic survivors (3) or the case of some Thorotrast patient analysis [a-particle emission (19)], but these cases are rare. Thus, the presence of several Dics certainly is useful information in analyzing exposure status.…”

“…However, Dic chromosomes are unstable with time after exposure (3,4) and a biological dosimetry based on their detection alone has limitations with regard to past overexposure. Problems may be encountered in dose reconstruction when the time between exposure and analysis is considerable or even unknown.…”

Breaking down cell cycle checkpoints and DNA repair during antigen receptor gene assembly

“…For example, symmetric exchanges such as reciprocal chromosomal translocations, associated with lymphomagenesis, have been observed to persist for many cell doublings in vitro (Kano and Little, 1984), for decades in peripheral blood lymphocytes from atomic bomb survivors (Awa et al, 1978) and in rapidly dividing mouse bone marrow cells (Spruill et al, 1996). These lesions are thought to be stable because they are not associated with any loss of genetic material, and the derivative chromosome, which carries a single centromere and ends with telomeric DNA, remains structurally intact.…”