Relationship between ultrastructural changes and EROD activities in liver of fish exposed to Benzo[a]pyrene

Au, Doris W.T.; Wu, Rongben; Zhou, B. S.; Lam, Paul K.S.

doi:10.1016/s0269-7491(98)00176-6

Cited by 88 publications

(48 citation statements)

References 51 publications

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“…Pronounced accumulation of lipofuscin in fish liver and mussel digestive gland has been associated with oxidative stress caused by pollution with PAHs (Krishnakumar et al, 1994(Krishnakumar et al, , 1997Au et al, 1999;Au, 2004). In our study, lipofuscin did not accumulate in phenanthrene-exposed gonads, consistent with a previous study where exposure to even 500 mg L À1 of phenanthrene for 20 days did not result in elevated amounts of lipofuscin in ovaries of P. miliaris (Schäfer and Köhler, 2009).…”

Section: Discussionsupporting

confidence: 91%

“…Lipofuscin, also known as fluorescent age pigment, is an end product of protein and lipid peroxidation which accumulates as an ultimate consequence of oxidative stress (Au et al, 1999;Au, 2004;Terman and Brunk, 2004). In mussels and fish, contamination with PAHs has been associated with a more pronounced accumulation of lipofuscin in polluted groups compared to control groups (Krishnakumar et al, 1994(Krishnakumar et al, , 1997Au et al, 1999;Au, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure

Schäfer

Abele

Weihe

et al. 2011

Marine Environmental Research

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a b s t r a c tFemale and male individuals of the same species often differ with respect to their susceptibility to toxicant stress. In the present study, sea urchins (Psammechinus miliaris) of both sexes were exposed to high (150 mg L À1 ) and environmentally relevant (5 mg L À1 ) concentrations of phenanthrene over 10 days. While food intake was significantly decreased following exposure to 150 mg L À1 phenanthrene, histological indices (lipofuscin accumulation, fibrosis, oocyte atresia), energetic status (energy charge, sum adenylates, AMP/ATP ratio) as well as ascorbate levels in the gonads showed either little or no effect upon phenanthrene exposure. However, most parameters (vitamin C, energy charge, sum adenylates, AMP/ATP ratio, ATP and ADP concentrations, lipofuscin content, fibrosis) significantly differed between male and female animals. This study illustrates the difficulties to identify toxic injury in reproductive tissue as it may be superimposed by gametogenesis and spawning of gametes.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure

Schäfer

Abele

Weihe

et al. 2011

Marine Environmental Research

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“…those found in seawater) that may interfere with the detection of elements Thin sections of Tetraselmis sp. cells were prepared for SR-XRF analysis by following the procedure used by Levy et al 6 and based on the methods of Au et al, 22 without the use of metalstaining agents normally used to prepare specimens for transmission electron microscopy (e.g. osmium tetroxide, uranyl acetate and lead citrate).…”

Section: Microalgalmentioning

confidence: 99%

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Adams

Dillon

Vogt

et al. 2016

Environ. Sci. Technol.

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Metal toxicity to aquatic organisms depends on the speciation of the metal and its binding to the critical receptor site(s) (biotic ligand) of the organism. The intracellular nature of the biotic ligand for Cu in microalgal cells was investigated using the high elemental sensitivity of microprobe synchrotron radiation X-ray fluorescence (SR-XRF) and X-ray absorption near-edge spectroscopy (XANES). The marine microalgae, Ceratoneis closterium, Phaeodactylum tricornutum, and Tetraselmis sp. were selected based on their varying sensitivities to Cu (72-h 50% population growth inhibitions of 8–47 μg Cu/L). Intracellular Cu in control cells was similar for all three species (2.5–3.2 × 10–15 g Cu/cell) and increased 4-fold in C. closterium and Tetraselmis sp. when exposed to copper, but was unchanged in P. tricornutum (72-h exposure to 19, 40, and 40 μg Cu/L, respectively). Whole cell microprobe SR-XRF identified endogenous Cu in the central compartment (cytoplasm) of control (unexposed) cells. After Cu exposure, Cu was colocated with organelles/granules dense in P, S, Ca, and Si and this was clearly evident in thin sections of Tetraselmis sp. XANES indicated coexistence of Cu(I) and Cu(II) in control and Cu-exposed cells, with the Cu ligand (e.g., phytochelatin) in P. tricornutum different from that in C. closterium and Tetraselmis sp. This study supports the hypothesis that Cu(II) is reduced to Cu(I) and that polyphosphate bodies and phytochelatins play a significant role in the internalization and detoxification of Cu in marine microalgae.

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“…For example, Au et al (1999) used injection concentrations ranging from 100 lg to 10 mg kg 21 of B[a]P in the fish Solea ovata, and did not detect any observable cytological changes in the liver at doses lower than 1 mg kg 21 . Injections with BNF in other studies ranged from 40 mg to 100 mg kg 21 (Raza et al, 1995;Stegeman et al, 1997;Novi et al, 1998;Weber and Janz, 2001;Teles et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Hepatic metabolism of contaminants in the terapontid fish, yellowtail trumpeter (Amniataba caudavittata Richardson)

Webb

Gagnon

Rose

2008

Environmental Toxicology

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The yellowtail trumpeter (Amniataba caudavittata) is an estuarine-dependent omnivorous fish found in the Swan-Canning Estuary, Western Australia. Thirty five fish were injected with either the polycyclic aromatic hydrocarbon, benzo[a]pyrene (B[a]P), the synthetic flavenoid b-naphthoflavone (BNF), or used as controls. The fish were then sampled at 3 and 7 days postinjection. Induction of the enzyme ethoxyresorufin O-deethylase (EROD) activity was nonsignificant while ethoxycoumarin O-deethylase (ECOD) activity induction differed amongst treatments. A high interindividual variability in the EROD activity was observed. The measurement of sorbitol dehydrogenase in the serum (s-SDH) was elevated (BNF 2.2 times and B[a]P 3.2 times the control fish) demonstrating that liver cell damage had occurred. Increases in biliary metabolites of both B[a]P-type and pyrene-type (19 times and 3.4 times the controls respectively) indicated that detoxification of pyrene-type compounds had taken place. Fish of the Terapontidae family, such as the yellowtail trumpeter, were found to be suitable for biomonitoring the health of the Swan-Canning Estuary. A combination of ECOD activity, s-SDH, and the measurement of biliary metabolites represents a suitable suite of biomarkers for environmental monitoring of the sublethal effects of PAH pollution in these fish. # 2008 Wiley Periodicals, Inc. Environ Toxicol 23: 68-76, 2008

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Relationship between ultrastructural changes and EROD activities in liver of fish exposed to Benzo[a]pyrene

Cited by 88 publications

References 51 publications

Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure

Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Hepatic metabolism of contaminants in the terapontid fish, yellowtail trumpeter (Amniataba caudavittata Richardson)

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