Relationship between ultrastructure and biomechanical properties of the knee meniscus

Gabrion, Antoine; Aimedieu, Patrick; Laya, Z.; Havet, É.; Mertl, P.; Grebe, Rhonda; M, Laude

doi:10.1007/s00276-005-0031-6

Cited by 39 publications

(29 citation statements)

References 16 publications

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“…Gabrion et al 23 did not report standard deviations; comparable coefficients of variation were seen in other tests using human menisci. Lechner et al 6 found coefficients for the circumferential tensile modulus ranging from 0.6 to 1.3 for a similarly sized sample as in our study.…”

Section: Discussionmentioning

confidence: 92%

Compressive moduli of the human medial meniscus in the axial and radial directions at equilibrium and at a physiological strain rate

Chia

Hull

2008

Journal Orthopaedic Research

177

145

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The axial and radial compressive moduli of the human meniscus are important material properties in tibiofemoral joint models, but they have not been determined previously for fresh-frozen tissue. Our goals were to measure the moduli at equilibrium and at a physiological strain rate, to determine whether the axial and radial compressive moduli are equal for each type of loading, and to determine whether they depend on the region (i.e., anterior, middle, posterior) of the meniscus. Samples from each region from 10 fresh-frozen human medial menisci were tested in unconfined compression at four strain levels (3%, 6%, 9%, and 12%) at 32%/s, a strain rate determined to be physiologically relevant to walking, and then allowed to reach equilibrium in stress relaxation. At equilibrium, the axial and radial compressive moduli at 12% strain were 83.4 kPa and 76.1 kPa, respectively (p ¼ 0.58), whereas at the physiological strain rate, the axial and radial compressive moduli at 12% strain were 718 kPa and 605 kPa, respectively (p ¼ 0.61). At the physiological strain rate, the modulus increased with increasing strain (79.2 kPa at 3% strain vs. 662 kPa at 12% strain) and the modulus in the anterior region (1,048 kPa at 12% strain) was significantly greater than that in the posterior region (329 kPa at 12% strain) (p ¼ 0.04). Our study supports a plane of isotropy for the material properties of meniscal tissue. However, the material behavior is strongly nonlinear because the compressive modulus is several orders of magnitude smaller than previously reported values for tensile modulus. Further, the compressive modulus depends on the activity of interest (i.e., static such as standing or dynamic such as walking) due to viscoelastic effects, the strain level, and the region of the tissue. ß

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Section: Discussionmentioning

confidence: 92%

Compressive moduli of the human medial meniscus in the axial and radial directions at equilibrium and at a physiological strain rate

Chia

Hull

2008

Journal Orthopaedic Research

177

145

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“…Similarly, increased inner meniscus cell expression of Col 2 also corresponds to the most abundant collagen protein found in the inner region. [31][32][33] These results suggest that upon liberation from their resident ECM, meniscus cells respond by upregulating genes commensurate with restoring their native environment. This behavior is different than that observed in freshly isolated articular chondrocytes, in which gene expression of Col 2 and AGC was unaffected or decreased following tissue digestion, providing more evidence of the distinction between articular chondrocytes and meniscus cells.…”

Section: Fig 3 Phase 2 Cell Yield and Live-dead Analysis (A)mentioning

confidence: 88%

“…The inner portion of the meniscus is similar to hyaline cartilage in that it contains the majority of sulfated GAGs and collagen type II (Col 2), whereas the middle and outer meniscus regions contain a higher proportion of collagen type I (Col 1). [29][30][31][32][33] The morphology of meniscus cells also becomes progressively more fibroblast-like peripherally in the meniscus, with the inner region cells more rounded and chondrocyte like and the outer cells containing more cellular processes. 34 In addition to morphology, regional cell phenotypic differences are observed; cells in the outer meniscus show high gene expression for Col 1, while cells from the inner region display high gene expression for Col 2 and aggrecan (AGC).…”

mentioning

confidence: 99%

Regional Effects of Enzymatic Digestion on Knee Meniscus Cell Yield and Phenotype for Tissue Engineering

Athanasiou

2012

Tissue Engineering Part C: Methods

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“…It is therefore reasonable to assume that circumferential tension (so-called ''hoop stress'') is a main component of the forces that act on the meniscus. 21 Jones et al 22 measured strains in the circumferential direction on human menisci by mounting miniature differential variable reluctance transducers at various sites of the meniscal periphery. They demonstrated that hoop strain is induced in the meniscus when the knee is subjected to simulated weight bearing.…”

Section: Discussionmentioning

confidence: 99%

Tensile forces at the porcine anterior meniscal horn attachment

Stärke

Kopf

Gröbel

et al. 2009

Journal Orthopaedic Research

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Tibiofemoral compression causes circumferential tension in the knee meniscus, which is transferred to the tibial bone at the anterior and posterior attachments. The objective of the study was to measure the resulting tensile forces at the horn attachment in a porcine model. The anterior horn attachment of the porcine medial meniscus (n ¼ 10) was separated from the surrounding bone with a core reamer. A force transducer was installed such that tensile forces acting upon the now mobile horn attachment could be measured. The tibiofemoral joint was loaded in compression, starting at a preload of 30 N, with three 150-N increments, giving 180, 330, and 480 N load. Flexion angles of 0, 30, and 608 were investigated. The average resultant tension at the horn attachment was 26.3, 40.6, and 55.4 N with full extension, 29.2, 47.8, and 62.2 N at 308 flexion and 30.1, 49.6, and 68.1 N at 608 flexion. The tibiofemoral compression had a significant effect on the tension (p < 0.001), whereas no influence of the flexion angle was found (p ¼ 0.291). The study demonstrates that tibiofemoral compressive loads cause considerable tensile forces at the anterior meniscal horn attachment. The data are of interest for models of the repair or replacement of the knee menisci. ß

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Relationship between ultrastructure and biomechanical properties of the knee meniscus

Cited by 39 publications

References 16 publications

Compressive moduli of the human medial meniscus in the axial and radial directions at equilibrium and at a physiological strain rate

Compressive moduli of the human medial meniscus in the axial and radial directions at equilibrium and at a physiological strain rate

Regional Effects of Enzymatic Digestion on Knee Meniscus Cell Yield and Phenotype for Tissue Engineering

Tensile forces at the porcine anterior meniscal horn attachment

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