Relationship between Urinary Liver-Type Fatty Acid-Binding Protein (L-FABP) and Sarcopenia in Spontaneously Diabetic Torii Fatty Rats

Tanabe, Jun; Ogura, Yuji; Kosaki, Keisei; Nagai, Yoshio; Sugaya, Takeshi; Ohata, Keiichi; Watanabe, Sumi; Ichikawa, Daisuke; Inoue, Kenji; Hoshino, Seiko; Kimura, Kenjiro; Maeda, Seiji; Shibagaki, Yugo; Kamijo‐Ikemori, Atsuko

doi:10.1155/2020/7614035

Cited by 9 publications

(13 citation statements)

References 34 publications

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“…According to an animal model for the analysis of diabetes-related sarcopenia [24], disruption to insulin/ insulin-like growth factor-1 (IGF-1)-Akt signaling due to insulin resistance and low serum IGF-1 levels reduced muscle protein synthesis and provoked abnormalities in muscle strength and mass. In addition, phosphorylation of adenosine monophosphate-activated protein kinase due to reduced cellular uptake of glucose leads to suppression of muscle protein synthesis [25]. The results of these studies suggest that elderly patients with diabetes would be less trainable for muscle strength compared with their healthy counterparts.…”

Section: Discussionmentioning

confidence: 97%

Effects of resistance training using elastic bands on muscle strength with or without a leucine supplement for 48 weeks in elderly patients with type 2 diabetes

et al. 2021

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Type 2 diabetes is associated with sarcopenia. Resistance training and appropriate nutritional therapy are reported to be effective for muscle strength and mass. This study aimed to evaluate the effect of resistance training using elastic bands at home combined with a leucine-rich amino acid supplement on muscle strength, physical function, and muscle mass in elderly type 2 diabetes. We conducted a 48-week prospective single-center randomized controlled trial in 60 patients who were randomly allocated to one of three groups: control (C), resistance exercise (R), and resistance exercise plus supplement (RL). R and RL groups performed daily bodyweight resistance training with elastic bands exercises at home, and the RL group also took 6 g of a leucine-rich amino acid supplement daily. Knee extension strength (muscle strength), grip strength, usual gait speed (physical function), muscle mass, and cognitive function were assessed at 0 and 48 weeks. Although the change in knee extension strength from baseline was significantly increased by 6.4 Nm (95% CI 1.0, 11.7) in the RL group (p = 0.036), no significant difference was observed among the three groups (p = 0.090). Physical function, muscle mass, and cognitive function also had no changes during the study period among the three groups. No additive effect of a leucine-rich amino acid supplement on muscle strength or mass was observed. Although a post hoc analysis comparing with or without resistance training (C group vs. R + RL group) found that knee extension strength was significantly increased (p = 0.028), and cognitive decline was less (p = 0.046) than in the C group.

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Section: Discussionmentioning

confidence: 97%

Effects of resistance training using elastic bands on muscle strength with or without a leucine supplement for 48 weeks in elderly patients with type 2 diabetes

et al. 2021

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“…Muscle strength was measured using the forelimb grip test with a grip strength meter (MK-380CM/FM; Muromachi Kikai, Co., Ltd., Tokyo, Japan). The average of 3 measurements of muscle strength per animal per time point was used for the comparative analysis [18].…”

Section: Animalsmentioning

confidence: 99%

“…Urine samples were collected for the measurement of urinary markers. Under inhalation, anesthesia using 2% isoflurane, serum and kidney and leg muscle tissue samples were collected after 16 h of starvation at age 16 weeks [18]. The extracted kidneys were weighed and cut into pieces for various downstream analyses.…”

Section: Animalsmentioning

confidence: 99%

“…Spontaneously diabetic Torii (SDT) fatty (SDT.Cg-Lepr fa /JttJcl) rats mimic the human pathophysiology of type 2 diabetes with obesity, hypertension, and hyperlipidemia [17]. In our previous study, SDT fatty rats showed symptoms of DKD and muscle weakness at 16 weeks of age [18,19]. Therefore, the aim of this study was to investigate the beneficial effects of endurance exercise training on the kidneys and muscle by using SDT fatty rats.…”

Section: Introductionmentioning

confidence: 99%

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Endurance Exercise Training-Attenuated Diabetic Kidney Disease with Muscle Weakness in Spontaneously Diabetic Torii Fatty Rats

Kotake

Yamada

Ogura

et al. 2021

Kidney Blood Press Res

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Background The aim of this study was to evaluate protective effects of endurance exercise training against diabetic kidney disease (DKD) with muscle weakness by using male spontaneously diabetic Torii (SDT) fatty rats as type 2 diabetic animal models with obesity, hypertension, and hyperlipidemia. Methods Eight-week-old SDT fatty rats (n = 12) and Sprague–Dawley (SD) rats (n = 10) were randomly divided into exercise (Ex; SDT-Ex: n = 6, SD-Ex: n = 5) and sedentary groups (SDT-Cont: n = 6, SD-Cont: n = 5), respectively. Each group underwent regular treadmill exercise four times a week from ages 8 to 16 weeks. Results The exercise attenuated hypertension and hyperlipidemia and prevented increases in renal parameter levels without affecting blood glucose levels. In the SDT fatty rats, it prevented induction of renal morphological abnormalities in the interstitium of the superficial and intermediate layers of the cortex. Downregulated expression of endothelial nitric oxide synthase in the glomerulus of the SDT fatty rats was significantly upregulated by the exercise. The exercise upregulated the renal expressions of both medium-chain acyl-CoA dehydrogenase and peroxisome proliferator-activated receptor γ coactivator-1α related to fatty acid metabolism. It increased muscle strength and both muscle weight and cross-sectional area of type IIb muscle fibers in the extensor digitorum longus muscle in the SDT fatty rats. Conclusion Endurance exercise training in type 2 diabetes ameliorates DKD by improving endothelial abnormality and enhancing fatty acid metabolism in addition to attenuated hypertension, hyperlipidemia, and muscle weakness independently of blood glucose levels.

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“…On the other hand, a recent meta-analysis and another clinical study did not show that GLP-1RA contributed to DR aggravation [10,11]; thus, the in uence of GLP-1RA on DR is uncertain in type 2 diabetes. Because the progression of DR may depend on various metabolic disorders in addition to hyperglycemia in patients with type 2 diabetes, we investigated the effect of liraglutide, a GLP-1RA, on DR in a rat model of type 2 diabetes using spontaneously diabetic Torii (SDT) fatty (SDT.Cg-Lepr fa /JttJcl) rats, which resemble the human pathophysiology of type 2 diabetes with hypertension, obesity, and hyperlipidemia [12,13].…”

Section: Introductionmentioning

confidence: 99%

Glucagon-Like Peptide-1 Receptor Agonist, Liraglutide, Attenuated Diabetic Retinal Edema in Spontaneously Diabetic Torii Fatty Rats

Inoue

Yamada

Hoshino

et al. 2021

Preprint

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Background: This study aims to investigate the effect of the glucagon-like peptide-1 (GLP-1) receptor agonist (GLP-1RA) liraglutide on retinal edema as compared with insulin and hydralazine using an animal model of type 2 diabetes with obesity, hypertension, and hyperlipidemia.Methods: Male spontaneously diabetic Torii (SDT) fatty rats at 8 weeks of age were randomly assigned to three groups: the liraglutide group (SDT-lira, n = 6) received a subcutaneous injection of liraglutide from the age of 8 to 16 weeks, the SDT-ins-hyd group (n = 6) was provided both insulin against hyperglycemia and hydralazine against hypertension to match levels of both blood glucose and blood pressure to those of the liraglutide group, and the control group of SDT fatty rats (SDT-vehicle, n = 7) and a nondiabetic control group of Sprague–Dawley rats (SD, n = 7) were injected with vehicle only. Both eyeballs of all groups were collected at the age of 16 weeks.Results: Retinal thickness, which was found in the SDT-vehicle group, was significantly prevented to similar levels in both the SDT-lira and SDT-ins-hyd groups. Immunohistological analysis revealed that GLP-1 receptor was not expressed in the retina of all rats. The ocular protein expression of monocyte chemoattractant protein-1, which causes a proinflammatory situation, was significantly upregulated in all SDT fatty rats as compared to SD rats, but the expression levels were similar between all SDT fatty rats. With regard to neovascularization in the eyes, there were no significant differences in protein expressions of vascular endothelial growth factor, CD31, or endothelial nitric oxide synthase in all rats.Conclusions: The present study indicates that liraglutide prevents retinal thickening, dependent on blood glucose and blood pressure levels in SDT fatty rats without ocular neovascularization. However, the effects did not improve the ocular proinflammatory state.

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Relationship between Urinary Liver-Type Fatty Acid-Binding Protein (L-FABP) and Sarcopenia in Spontaneously Diabetic Torii Fatty Rats

Cited by 9 publications

References 34 publications

Effects of resistance training using elastic bands on muscle strength with or without a leucine supplement for 48 weeks in elderly patients with type 2 diabetes

Effects of resistance training using elastic bands on muscle strength with or without a leucine supplement for 48 weeks in elderly patients with type 2 diabetes

Endurance Exercise Training-Attenuated Diabetic Kidney Disease with Muscle Weakness in Spontaneously Diabetic Torii Fatty Rats

Glucagon-Like Peptide-1 Receptor Agonist, Liraglutide, Attenuated Diabetic Retinal Edema in Spontaneously Diabetic Torii Fatty Rats

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