Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor

Shagawa, Tomohiro; Torii, Hiroomi; Kato, Fumihito; Ogi, Hirotsugu; Hirao, Masahiko

doi:10.7567/jjap.54.068001

Cited by 11 publications

(19 citation statements)

References 15 publications

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“…We assume that a continuous and homogenous protein layer grows on the quartz oscillator between the solution layer and quartz surface. The resonance frequency of such a multilayer system principally depends on four parameters: thickness, viscosity, stiffness of the protein layer, and the overtone index n of the resonance. ,, We then simultaneously measured the frequency change for n = 1, 3, 5, and 7 to extract the remaining three parameters inversely by the least-squares method.…”

Section: Viscoelastic Modelmentioning

confidence: 99%

“…In the model, , we assigned different materials to each layer: first, the WE-QCM is regarded as purely elastic (α-quartz). Next, the protein (Aβ) layer is regarded as a viscoelastic Kelvin–Voigt material, where an elastic spring and a dashpot are connected in parallel.…”

Section: Viscoelastic Modelmentioning

confidence: 99%

“…16,17 This is because the amyloid fibrils show stiffness much higher than standard protein aggregates. 18 Because a high-frequency quartz-crystal microbalance (QCM) is sensitive not only to the mass of the aggregates on the surface but also to their viscoelasticity, 19,20 we expect that such a structural transition can be detected with a high-frequency QCM measurement. Viscoelastic models in a QCM assay 21−23 have been built for various surroundings such as gas, water, 24,25 and amphiphilic overlayer.…”

Section: ■ Introductionmentioning

confidence: 99%

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Viscoelasticity Response during Fibrillation of Amyloid β Peptides on a Quartz-Crystal-Microbalance Biosensor

et al. 2018

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Unlike previous in vitro measurements where Amyloid β (Aβ) aggregation was studied in bulk solutions, we detect the structure change of the Aβ aggregate on the surface of a wireless quartz-crystal-microbalance biosensor, which resembles more closely the aggregation process on the cell membrane. Using a 58 MHz quartz crystal, we monitored changes in the viscoelastic properties of the aggregate formed on the quartz surface from monomers to oligomers and then to fibrils, involving up to the 7th overtone mode (406 MHz). With atomic-force microscopy observations, we found a significant stiffness increase as well as thinning of the protein layer during the structure change from oligomer to fibrils at 20 h, which indicates that the stiffness of the fibril is much higher. Viscoelasticity can provide a significant index of fibrillation and can be useful for evaluating inhibitory medicines in drug development.

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Section: Viscoelastic Modelmentioning

confidence: 99%

Section: Viscoelastic Modelmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Viscoelasticity Response during Fibrillation of Amyloid β Peptides on a Quartz-Crystal-Microbalance Biosensor

et al. 2018

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“…Second, we study the viscoelasticity change during the binding reaction using a MEMS wireless QCM biosensor 20,21) by measuring resonance-frequency changes up to 9th mode (522 MHz), 22) through which the viscoelastic parameters are inversely determined. 23,24) 2. Experiments We then injected human IgG in PBS solutions of various concentrations at a flow rate of 100 µl/min.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of affinity-enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor

Noi

Iijima

Kuroda

et al. 2020

Jpn. J. Appl. Phys.

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Recent advances in functionalized bio-nanocapsules (BNCs) allow significant sensitivity enhancement in label-free biosensors. It is suggested that the sensitivity amplification is caused by higher binding affinity of BNC to target antibody. We here study the high-affinity interaction mechanism between BNC and antibody with wireless high-frequency QCM biosensors. We first confirm higher affinity between BNC and human immunoglobulin G. We then reveal that the number of the binding sites for the target antibody highly increases by immobilizing BNC molecules on the sensor surface. We finally study the change in the viscoelasticity near the sensor surface using the MEMS wireless QCM biosensor using up to 9th overtone (522 MHz). The inversely determined effective shear modulus on BNC is found to be significantly lower than the standard surface on which the receptor molecules are immobilized. We thus clarify that this surface flexibility will achieve the high affinity with the target antibody.

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“…Using this maximum thickness and the total mass deposited of 30 µg cm −2 , we can estimate a density of 0.49 g cm −3 , in agreement with Bawazer et al [ 16 ] However, the deposited mass derived in this way is an underestimation due to viscoelastic effects in thick layers. [ 18,19 ] Analysis using the Kelvin–Voigt Model (Figure S3, Supporting Information), that takes these effects into account, leads to a larger deposited mass of 35 µg cm −2 , corresponding to a density of 0.58 g cm −3 . The densities of nanoparticulate TiO 2 and of papain typically are 4.2 and 1.2 g cm −3 , [ 16,18,20 ] suggesting the papain/titania layer is not as dense as previously described, [ 16 ] but is actually highly porous.…”

Section: Resultsmentioning

confidence: 92%

Characterization of Enzymatically Synthesized Titania Thin Films Using Positron Annihilation Spectroscopy Reveals Low‐Cost Approach for Organic/Inorganic Photovoltaic Cells

Amelrooij

Schüt

Egger

et al. 2020

Advanced Sustainable Systems

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A new method is developed to produce mesoporous titania thin films at room temperature using the enzyme papain in a dip‐coating procedure, providing low‐cost titania films in a sustainable manner. Quartz crystal microbalance, positron annihilation Doppler broadening and lifetime spectroscopy, scanning electron microscopy, and X‐ray diffraction are used to determine the deposition and structural properties of the films. As‐deposited films have low densities ρ ≈ 0.6 g cm−3, contain small micropores and proteins, and exhibit corrugated surfaces. Annealing at temperatures of 300 °C or higher leads to the destruction and evaporation of most of the organic material, resulting in a thickness decrease of 50–60%, more pure titania films with increased density, an increase in micropore size and a decrease in the concentration and size of atomic‐scale vacancies. Up to 50 layers could be stacked, allowing easy control over the total layer thickness. Based on these titania films, first test devices consisting of natural dye‐sensitized solar cells are produced, that show photovoltaic activity and indicate possibilities for low‐cost, accessible, organic production of solar cells. Given the wide range of other applications for titania, this new method is a promising candidate for improving the fabrication of those products with respect to cost, sustainability, and production speed.

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Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor

Cited by 11 publications

References 15 publications

Viscoelasticity Response during Fibrillation of Amyloid β Peptides on a Quartz-Crystal-Microbalance Biosensor

Viscoelasticity Response during Fibrillation of Amyloid β Peptides on a Quartz-Crystal-Microbalance Biosensor

Mechanism of affinity-enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor

Characterization of Enzymatically Synthesized Titania Thin Films Using Positron Annihilation Spectroscopy Reveals Low‐Cost Approach for Organic/Inorganic Photovoltaic Cells

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