Relationship of abomasal histology and parasite-specific immunoglobulin A with the resistance to Haemonchus contortus infection in three breeds of sheep

Amarante, Alessandro Francisco Talamini do; Bricarello, Patrízia Ana; Huntley, John F.; Mazzolin, Lucilene Patrícia; Gomes, J.C.

doi:10.1016/j.vetpar.2004.11.021

Cited by 86 publications

(61 citation statements)

References 30 publications

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“…The results were consistent with previous reports. A close association between abomasum IgA and worm burdens was observed (Amarante et al 2005). Similar results were reportedly associated with reduced percentage L3 for O. circumcincta in sheep (Stear et al 1995), and O. ostertagi in calves (Geldhof et al 2002).…”

Section: Discussionsupporting

confidence: 81%

DNA vaccine encoding Haemonchus contortus Actin induces partial protection in Goats

Ren

Wang

et al. 2014

Acta Parasitologica

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Actin is a globular multi-functional protein that forms microfilaments, and participates in many important cellular processes. Previous study found that Haemonchus contortus actin could be recognized by the serum of goats infected with the homology parasite. This indicated that H. contortus actin could be a potential candidate for vaccine. In this study, DNA vaccine encoding H. contortus actin was tested for protection against experimental H. contortus infections in goats. Fifteen goats were allocated into three trial groups. The animals of Actin group were vaccinated with the DNA vaccine on day 0 and 14, and challenged with 5000 infective H. contortus third stage larval (L3) on day 28. An unvaccinated positive control group was challenged with L3 at the same time. An unvaccinated negative control group was not challenged with L3. The results showed that DNA vaccine were transcribed at local injection sites and expressed in vivo post immunizations respectively. For goats in Actin vaccinated group, higher levels of serum IgG, serum IgA and mucosal IgA were produced, the percentages of CD4 + T lymphocytes, CD8 + T lymphocytes and B lymphocytes and the concentrations of TGF-β were increased significantly (P<0.05). Following L3 challenge, the mean eggs per gram feces (EPG) and worm burdens of Actin group were reduced by 34.4% and 33.1%, respectively. This study suggest that recombinant H. contortus Actin DNA vaccine induced partial immune response and has protective potential against goat haemonchosis.

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Section: Discussionsupporting

confidence: 81%

DNA vaccine encoding Haemonchus contortus Actin induces partial protection in Goats

Ren

Wang

et al. 2014

Acta Parasitologica

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“…But in contrast to studies in Texel and Castellana sheep showing a stronger IgG and IgA response in animals previously exposed to infection (Schallig et al, 1995;Gomez-Munoz et al, 1999), in Creole kids naturally parasitized on pasture before the experimental infection, a weak response was observed except for IgA anti-ESP which showed amounts similar to primo-infected sheep. Previous studies in sheep suggest that IgA may be the major mechanism controlling fecundity of H. contortus and worm length and fecundity of Teladorsagia cicumcincta (Stear et al, 1995b;Strain et al, 2002 (Amarante et al, 2005). A significant IgA response was observed in both groups against H. contortus, but no relationship was found between IgA anti-L3 or IgA anti-ESP and FEC.…”

Section: Discussionmentioning

confidence: 70%

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

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The objective of this study was to evaluate the relationship of parasite-specific serum antibodies with the resistance status of Creole kids. The average breeding values on egg output predicted in a context of natural infection at 11 months of age were distant of 1.07 genetic standard deviation between resistant and susceptible animals. After drenching the animals were maintained worm-free during 1 month until experimental infection with 10,000 Haemonchus contortus infective larvae (L3). Enzyme-linked immunosorbent assay was carried out in serum samples to determine the level of IgG, IgA and IgE anti-H. contortus L3 crude extracts and adult excretion/secretion products (ESP). Parasitological and blood immunological parameters were measured on the 2 extreme groups. Despite the absence of any typical signs of haemonchosis, susceptible kids had more than 11 times higher faecal egg counts (FEC) at 35 days post-infection (d.p.i.) than resistant kids had. Levels of immunoglobulin against H. contortus L3 and ESP increased significantly after infection in both groups. However, no difference in the host immune response mediated by immunoglobulin against H. contortus was evidenced between groups. This finding suggests that, in goats previously infected by H. contortus, a degree of protection occurred and the phenotypic and genetic segregation in resistant and susceptible animals were not related to the humoral immune response. The correlation coefficients between FEC and IgE anti-ESP (r = 0.593; P < 0.05 was significant in both resistant and susceptible animals. Such correlation suggesting a hypersensitivity reaction dependent on worm prolificacy has never been described. This result needs further studies to understand the mechanisms underlying this observation. #

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“…There were no differences in hematological and biochemical parameters between the groups, which may have been due to the improvement of physiological conditions after anthelmintic treatment. A steady leukocyte count response in cases of gastrointestinal nematode infection, especially regarding abomasal eosinophil counts, was observed by Amarante et al, (2005) and Terefe et al, (2007) when studying sheep that were resistant and susceptible to H. contortus infection. This was seen because mobilization of effector cells is important in the early days of infection (TEREFE et al, 2009).…”

Section: Discussionmentioning

confidence: 93%

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

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Galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and Gal14) and collectins (SPA and CGN) in sheep. Twelve Corriedale sheep exposed to natural infection with nematodes were divided into two groups: group 1 (G1, n = 7) and group 2 (G2, n = 5), with low and high parasite burdens, respectively, based on fecal egg counts and abomasal parasite counts. The fecal egg counts and abomasal parasite counts were significantly different (p < 0.05) between the groups. Galectin and collectin gene expression was observed in all sheep abomasal samples. However, animals with lower infection levels showed lower expression of the genes Gal14, SPA and CGN (p < 0.05). Expression of lectins was associated with the abomasal H. contortus burden, thus suggesting that these proteins may have a role in controlling of this infection.Keywords: Innate immunity, lectins, helminths, small ruminants, PCR. ResumoColectinas e galectinas são proteínas da família das lectinas que possuem a capacidade de reconhecer padrões moleculares associados aos patógenos. Estudos em bovinos têm demonstrado a alta expressão dessas proteínas durante a infecção por nematoides gastrintestinais. O objetivo deste estudo foi investigar se o nível de infecção de Haemonchus contortus altera a expressão de colectinas (SPA e CGN) e galectinas (Gal11 e Gal14) de ovinos. Doze ovinos da raça Corriedale expostos a infecção natural com nematoides foram separados em dois grupos: grupo 1 (G1, n=7) com menor grau de parasitismo; e grupo 2 (G2, n=5) com maior grau, a partir da contagem do número de parasitos recuperados do abomaso e OPG. A contagem de OPG e de parasitos recuperados do abomaso dos grupos G1 e G2 apresentaram diferença estatística (p<0,05). A expressão dos genes de colectinas e galectina foi observada em todas as amostras de abomaso dos ovinos, porém animais com menor grau de infecção apresentaram menor expressão dos genes de Gal14, SPA e CGN (p<0,05). A expressão de lectinas foi associada ao número de H. contortus encontrados no abomaso de ovinos, indicando um possível papel dessas proteínas no controle da infecção.Palavras-chave: Imunidade inata, lectinas, helmintos, pequenos ruminantes, PCR.

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Relationship of abomasal histology and parasite-specific immunoglobulin A with the resistance to Haemonchus contortus infection in three breeds of sheep

Cited by 86 publications

References 30 publications

DNA vaccine encoding Haemonchus contortus Actin induces partial protection in Goats

DNA vaccine encoding Haemonchus contortus Actin induces partial protection in Goats

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

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