Background: This study protocol specifies the primary research line and theoretical framework of the 2023 Survey of the Psychology and Behavior on Chinese Population. It aims to establish a database focused on psychology and behavior among Chinese residents through a multicenter, large-sample cross-sectional survey to provide strong data support for research and development in related fields. It will track the public's physical and psychological health more comprehensively and systematically. Methods: The study was conducted from 20 June 2023 to 31 August 2023, using stratified and quota sampling methods, and a total of 150 cities, 202 districts and counties, 390 townships/streets, and 800 communities/villages were surveyed (excluding Taiwan, which were selected from 23 provinces, five autonomous regions, and four municipalities directly under the central government in China). The questionnaires were distributed face-to-face by trained surveyors. The questionnaires included basic information about the individual, personal health status, basic information about the family, the social environment in which the individual lives, psychological condition scales, behavioral level scales, other scales, and attitudes towards topical social issues. Supervisors conducted quality control during the distribution process and returned questionnaires, logically checked and cleaned for data analysis. Discussion: Data collection has been finished, and scientific outputs based on this data will support the development of health promotion strategies in China and globally. In the aftermath of the pandemic, it will guide policymakers and healthcare organizations to improve their existing policies and services to maximize the physical and mental health of the Chinese population. Keywords: Chinese residents, Physical and psychological health, Health behavior, Cross-sectional survey