Relationship of capsular type to biochemical and immunological properties of teichoic acid preparations from unencapsulated strains of Staphylococcus aureus

Ohtomo, Toshichika; Yoshida, Kenichi; Clemente, C. L. San

doi:10.1128/iai.14.5.1113-1118.1976

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…These results matched the findings obtained by electronmicroscopy (Yoshida & Minegishi 1975). The relationship of capsular-type to biochemical and immunological properties of cell wall teichoic acid preparations reported by Ohtomo et al (1976) agree with these findings especially those obtained with unencapsulated strains. Concerning the population of encapsulated strains of Staph.…”

Section: Discussionsupporting

confidence: 89%

Application of Fluorescent Antibody for Detecting Capsular Substances in Staphylococcus aureus

Yoshida

Takahashi

Ohtomo

et al. 1979

Journal of Applied Bacteriology

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A fluorescent antibody technique was developed for the determination of the capsular‐type of strains of Staphylococcus aureus. It compared favourably with the method using serum‐soft agar (Yoshida 1972). With the new technique, many populations of encapsulated and unencapsulated strains were investigated. Of 1421 fresh isolates of Staph. aureus, 54 were encapsulated and among these 54·8% and 48·1% were mono‐ and polyvalent, respectively. Capsular‐type antigens A and B were found in 92·5% and 44·4% of strains respectively; capsular‐types C and D were found relatively infrequently. In the other group, of unencapsulated strains, capsular‐type antigen production was demonstrated in 125 out of 163 strains examined. Mono‐ and polyvalent capsular‐types (A and B antigen producing strains) comprised 77·6% and 22·4%. respectively. In these capsular‐types A and B were found in 54·4% and 62·4%, respectively: capsular‐type antigen C and D producing strains were again infrequent. These results indicate that a majority of ordinary Staph. aureus strains produce capsular‐type antigens although isolation of the encapsulated strains is infrequent.

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Section: Discussionsupporting

confidence: 89%

Application of Fluorescent Antibody for Detecting Capsular Substances in Staphylococcus aureus

Yoshida

Takahashi

Ohtomo

et al. 1979

Journal of Applied Bacteriology

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“…Kelly and Weed (11) and Liau et al (13) found taurine to be a unique constituent of the cell wall or cell surface of the microorganisms. However, no one has observed the presence of taurine in the cell wall of S. aureus (23). We have shown that a crude enzyme preparation of cysteic acid decarboxylase can catalyze the formation of taurine in vitro.…”

Section: Discussionmentioning

confidence: 80%

Effect of bile acid derivatives on taurine biosynthesis and extracellular slime production in encapsulated Staphylococcus aureus S-7

Ohtomo¹,

Yoshida²,

Clemente³

1981

Infect Immun

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Various bile acids were added to cultures of encapsulated strains of Staphylococcus aureus growing in serum-soft agar medium of brain heart infusion broth. We examined effects of these compounds on cellular characteristics such as growth type, cell volume index, clumping factor reaction, slime yield, taurine content, and L-(--)-cysteic acid decarboxylase activity. Upon addition to the medium of either taurochenodeoxycholic acid, taurocholic acid (25 to 50 microgram/ml), or cholic acid (10 to 25 microgram/ml), the colonial morphology of taurine-positive cells (strain S-7) was altered from the diffuse to the compact type in serum-soft agar. Also, the titer of the clumping factor reaction increased, while the cell volume index and slime yield were markedly decreased. Tauro-bile acids, including taurocholic acid, taurochenodeoxycholic acid, taurodehydrocholic acid, and taurodeoxycholic acid (50 microgram/ml) inhibited the synthesis of taurine and resulted in decreased L-(--)-cysteic acid decarboxylase activity. Among all of the derivatives cholic acid itself was found to inhibit slime production and L-(--)-cysteic acid decarboxylase activity to the greatest extent. Glyco-bile acid derivatives and taurolicholic acid (50 to 100 microgram/ml) had no effect on L-(--)-cysteic acid decarboxylase activity. Compounds such as glycodeoxycholic acid (50 to 100 microgram/ml) had no effect upon any of the cellular characteristics tested. No effect was observed upon addition of any of these compounds to cultures of the taurine-negative strain (T-26-B). We did find a correlation between the inhibition of taurine biosynthesis and decreased slime production. Electron micrographs indicated that this encapsulated strain was converted to an unencapsulated state in the presence of bile acids.

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“…Adhesion with soybean lectin, in particular, is likely to be affected by changes in the receptor because of cellular damage on the cell surface polysaccharide (14,35). It is known that the unencapsulated strain does not contain N-acetylgalactosamine in the cell surface fraction (14,19). Also, the ability of the encapsulated strain S-7 to adhere to fibrinogen increased after freeze-drying treatment.…”

Section: Discussionmentioning

confidence: 99%

Outermost-cell-surface changes in an encapsulated strain of Staphylococcus aureus after preservation by freeze-drying

Ohtomo

Yamada

Yoshida

1988

Appl Environ Microbiol

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The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular characteristics such as growth type in serum-soft agar, cell volume index, and clumping factor reaction were examined. After freeze-drying the colonial morphology of strain S-7 was altered from a diffuse to a compact type in serum-soft agar. In accordance with these changes, the titer of the clumping factor reaction increased while the cell volume index, capsule and slime production, and capsule antigen production were markedly decreased in parallel with the period of freeze-drying. The ability of the strain to adhere to collagen, fibrinogen, and soybean lectin was also compared before and after freeze-drying. Fibrinogen levels slightly increased when 10% skim milk and 2% honey were used as cryoprotective agents and showed a remarkable increase when 0.05 M phosphate buffer was used as a control. Also, the ability of strain S-7 to adhere to soybean lectin declined, whereas no changes were observed for collagen under any conditions. Strain S-7 was phage nontypable before freeze-drying but the number of typable cells increased after freeze-drying; phage-typable cells reacted to phage 52 alone after 5 h of freeze-drying, but additional cells also proved to be phage typable to phage 42E after 10 h. Electron micrographs indicated that strain S-7, an encapsulated strain, was converted to an unencapsulated state after freeze-drying. Results of our study indicate that the freeze-drying process inhibits capsule and slime production in S. aureus, which consequently brings about changes in the outermost cell surface.

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Relationship of capsular type to biochemical and immunological properties of teichoic acid preparations from unencapsulated strains of Staphylococcus aureus

Cited by 8 publications

References 27 publications

Application of Fluorescent Antibody for Detecting Capsular Substances in Staphylococcus aureus

Application of Fluorescent Antibody for Detecting Capsular Substances in Staphylococcus aureus

Effect of bile acid derivatives on taurine biosynthesis and extracellular slime production in encapsulated Staphylococcus aureus S-7

Outermost-cell-surface changes in an encapsulated strain of Staphylococcus aureus after preservation by freeze-drying

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