Relationship of Hairless Mouse Skin Surface Temperature to Wound Severity and Maturation Time

Govil, Sharad Kumar; Flynn, Allen J.; Flynn, Gordon L.; Ackermann, Chrisita

doi:10.1159/000072071

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…Another reason could be the massive dermatoliposclerosis, leading to a prolonged diffusion distance due to a perivascular fibrosis and sclerosis 26 . The decrease in skin temperature following injury has been demonstrated as an indication of skin barrier impairment in mouse models 27 . In horses, wound temperature was shown to be lower in limb than in trunk wounds throughout the healing process 28 .…”

Section: Discussionmentioning

confidence: 99%

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Dynamic optical coherence tomography of chronic venous ulcers

Vélez González,

Berger,

Schiele

et al. 2023

Acad Dermatol Venereol

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BackgroundChronic ulcers, especially venous leg ulcers, are a major burden on the healthcare system. To date there are only few non‐invasive established procedures for evaluation of blood perfusion in wounds. Dynamic optical coherence tomography (D‐OCT) provides images of the skin's superficial vascularization.ObjectivesThis study aims to investigate if and how the D‐OCT measurement of chronic wounds can provide new information about the vascularization during the healing process.MethodsWe examined 16 venous ulcers over 16 weeks und evaluated the vessel morpho‐logy and density using D‐OCT at the wound bed, borders, two centimeters adjacent to the wound und at non‐ulcerated skin on the contralateral leg.ResultsIn D‐OCT scans clumps were unique and the most common vessel type in the wound area of venous ulcers, whereas lines and serpiginous vessels were the most common in non‐ulcerated skin. At the wound border mottle and cluster patterns occurred more frequently. Healthy skin showed a significant increase of mesh pattern. Vessel density significantly increased at the wound area compared to non‐ulcerated skin. During the healing process the wound border showed the most vascular changes while only an increase in curves was observed in the wound centre. Non‐healing wounds had fewer dots and blobs at the borders, fewer dots, coils, clumps, lines and serpiginous vessels at the centre, and fewer dots in adjacent skin. Temperature analysis showed higher temperatures in non‐ulcerated skin, followed by the wound margin and centre. Non‐healing wounds showed the lowest temperatures in the wound centre.ConclusionsThese results highlight the non‐invasive use of D‐OCT for the examination and monitoring of wound healing in chronic venous ulcers. D‐OCT imaging of blood vessels may offer the potential to detect disorders of wound healing at an early stage, differentiate ulcers of different genesis and to tailor more individualized, patient‐oriented therapy.

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Section: Discussionmentioning

confidence: 99%

“…26 The decrease in skin temperature following injury has been demonstrated as an indication of skin barrier impairment in mouse models. 27 In horses, wound temperature was shown to be lower in limb than in trunk wounds throughout the healing process. 28 Therapeutic approaches like temperatureactivated dressings could be of great importance.…”

Section: Dotsmentioning

confidence: 99%

Dynamic optical coherence tomography of chronic venous ulcers

Vélez González,

Berger,

Schiele

et al. 2023

Acad Dermatol Venereol

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“…Gordon completed his remarkable career in skin science with papers on the transport of thalidomide derivatives across human skin with his South African colleagues, led by Jeanetta Du Plessis [65], and on wound repair [66]. The latter work performed on hairless mouse skin showed that the stratum corneum permeation by ethanol is not impaired by heat until the temperature rises over 70°C.…”

Section: Gordon Flynn - Experimental Verification and Development Of mentioning

confidence: 99%

Solute-Vehicle-Skin Interactions in Percutaneous Absorption: The Principles and the People

Roberts¹

2013

Skin Pharmacol Physiol

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An appreciation of solute-vehicle-skin interactions underpins our current understanding of the processes of percutaneous absorption as well as in the prediction of the extent of absorption. This understanding has been reached through principles developed and validated over the last century through the work of a number of authors, including Dale Wurster, Takeru Higuchi, Irvin Blank, Robert Scheuplein, Gordon Flynn, Boyd Poulsen and Tom Franz, as well as by many scientists from my and younger generations. Their work has led to an appreciation of the rate-limiting steps in percutaneous penetration, the role played by the physicochemical properties of the solute, vehicle and skin and the variability that may arise from using various experimental/mathematical/pharmacokinetic models to quantify absorption as well as enabling the prediction of local and systemic efficacy and toxicity. In addition, unexpected behaviour may result from non-ideality in solute-vehicle-skin effects, including dehydration, chemical enhancement, supersaturation, metabolism, sequestration and vascular effects, including those of nanosystems on the local vasculature. In general, in vitro skin penetration profiles are predictive of in vivo profiles but a number of exceptions also exist.

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Polyvinyl alcohol nanofiber formulation of the designer antimicrobial peptide APO sterilizes Acinetobacter baumannii-infected skin wounds in mice

et al. 2015

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Native and designer cationic antimicrobial peptides are increasingly acknowledged as host defense molecules rather than true antimicrobials. Due to their ability to activate the innate immune system, these structures are used to treat uninfected and bacterially-infected wounds, including those harboring Acinetobacter baumannii. Previously we documented that when administered intramuscularly or topically in liquid formulations, the proline-rich host defense peptide dimer A3-APO accelerates uninfected wound re-epithelization and eliminates systemic and local A. baumannii, methicillin-resistant Staphylococcus aureus and other pathogen load from infected lesions better than conventional antibiotics. In the current study we sought to produce and characterize a novel delivery system, suitable for immediate and convenient application in non-hospital environments. The APO monomer was incorporated into polyvinyl alcohol nanofibers and the complex was polymerized into a solid patch dressing. Mice were subjected to skin abrasion where the wounds were either left uninfected or were inoculated with a near lethal dose of multidrug resistant A. baumannii strain. Analyzed after 3 days, APO monomer-containing patches improved wound appearance significantly better than polymer patches without antibiotics. When compared to colistin, the APO patches accelerated wound healing, and statistically significantly reduced wound size and wound bacterial load. The in vivo antimicrobial effect was more extensive than after intramuscular administration of the peptide drug, by using only one tenth of the active pharmaceutical ingredient. These data suggest that the APO monomer-impregnated nanofiber dressing can be developed as an economical first-line treatment option to skin injuries in general and battlefield burn and blast injuries in particular.

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Relationship of Hairless Mouse Skin Surface Temperature to Wound Severity and Maturation Time

Cited by 4 publications

References 3 publications

Dynamic optical coherence tomography of chronic venous ulcers

Dynamic optical coherence tomography of chronic venous ulcers

Solute-Vehicle-Skin Interactions in Percutaneous Absorption: The Principles and the People

Polyvinyl alcohol nanofiber formulation of the designer antimicrobial peptide APO sterilizes Acinetobacter baumannii-infected skin wounds in mice

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