This study comprised descriptive research the life stress and user satisfaction of female students who use University health clinic. From October 29 to November 11 2014, the 203 data were analyzed using t-test, ANOVA and Scheffe with SPSS 21.0. The highest score were in 'study', the lowest were in 'friend'. There were significant different in 'Future' and 'study' by major departments, 'friend' different by grade. 'Lover' was higher in drinking group, in total and 'value' were higher by meal habit, in 'friend' was higher in group binge. The motivations of use were high in medical treatment and InBody.Most satisfaction item were free administration and emergency medical treatment. Thus it is necessary to different design to reduce life stress in female university students and to operate effectively in university health clinic.•