Relationship of Mixed Lymphocyte Culture Response to Hl-a Histocompatibility Antigens

Abstract

It is now well established that compatibility for the t t L -A antigens between donor and host siblings is generally associated with prolonged excellent function of renal allografts (1-6). Although it may not be possible to identify all of the possible antigens of both segregating series in transplant pairs, nonreactive mixed lymphocyte cultures (MLC) 1 have proven to be excellent predictors of HL-A compatibility and graft function.Controversy remains, however, as to what criteria of compatibility determined either by serologic typing (1 6) for H L -A antigens or degree of stimulation in MLC (1, 2, 7-13) can be relied upon to predict the next level of comparatively good transplant success. In this regard, Bach and others (2, 14-16) have stressed that within families donor-host compatibility for one allele is usually associated with intermediate stimulation in MLC as compared with two-allele compatibility or disparity. Seigler et al. (7), however, have noted rather remarkable overlap between the MLC responses between one-and two-allele mismatched cell donors.We have noted the same variability, but now report that a subgroup with low MLC stimulation may be distinguished bv the fact that the donor and host pair share one allele plus one antigen of the second unmatched allele as determined by lymphocytotoxicity testing for HL-A antigens. Materials and Methods16 families with at least four members were included in which genotyping for two segregating series of the HL-A locus was established on the basis of positive identity of at least three and sometimes four antigens. Serologic typing and MLC tests were all performed as a part of the work-up of one family member as a potential renal transplant recipient. The potential *Supported by National Institutes of Health grant AI-09716-01 and the Veterans Administration.1 Abbreviations used in this paper:

Comparison of antibacterial and antiimmune effects of certain rifamycins

“…As shown in Table 2, rifamide, in a daily dose of 200 mg/kg, had no detectable suppressive activity after 14 days. Rifampin, rifamycin SV, and 8 methylrifamycin appeared to be approximately equal in activity in this immunological model and produced a significant reduction in footpad swelling after 14 days of therapy.…”

“…The effects of the rifamycins studied on mitogen-induced blastogenesis were assayed by using human peripheral blood leukocytes. Blood was drawn from healthy donors into heparinized tubes, and the leukocytes were isolated and cultured by the method of Thompson et al (14) and by the microculture technique with 5 Because it was also considered possible that the effect of the rifamycirm on mitogen-induced blastogenesis could be the result of binding of the mitogen by the rifamycins, two experiments were conducted to assess this possibility. Rifamycin SV (one of the more active compounds studied) at 100 jig/ml was passed alone or together with 50 pg of Con A per ml through Sephadex G-25 (Pharmacia Fine Chemicals, Uppsala, Sweden), packed in a column 1 by 20 cm with phosphate-buffered saline (pH 7.0).…”

Mixed Lymphocyte Cultures in a Family with an LD Allele Shared by the Parents

“…The original concept that MLC activation in humans is dependent on serologically defined HL-A antigens (Amos & Bach 1968, Sorensen & Kissmeyer-Nielsen 1969 has recently been questioned. Increasing evidence now indicates that MLC stimulation is induced by lymphocyte defined (LD) histocompatibility antigens, which are the product of a locus closely linked to, but distinct from, the second HL-A locus (Bach 1973, Dausset et al 1973, Eijsvoogel et al 1972a,b, Gatti et al 1971, Hirschberg & Thorsby 1973, Thompson et al 1973, van Rood 1973, Yunis & Amos 1971.…”