Relationship of mouse minor satellite DNA to centromere activity

Broccoli, Dominique; Miller, O.J.; Miller, Dorothy A.

doi:10.1159/000132989

Cited by 53 publications

(31 citation statements)

References 14 publications

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“…To determine the physiological relevance of heterochromatin anchoring to the nucleolus, we performed enucleolation (Ogushi et al, 2008) and then examined its impact on chromatin organization in parthenotes or zygotes by using antibodies against histone H3 tri-methylated at lysine 9 (H3K9me3), which marks maternal chromatin (Arney et al, 2002) and pericentromeric heterochromatin (Probst et al, 2007), and CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia) autoimmune serum which labels mouse centromeres (Broccoli et al, 1990). For whole-DNA staining, 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) was used.…”

Section: Resultsmentioning

confidence: 99%

Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM2

Ogushi

Yamagata²,

Obuse

et al. 2017

Journal of Cell Science

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The mammalian oocyte nucleolus, the most prominent subcellular organelle in the oocyte, is vital in early development, yet its key functions and constituents remain unclear. We show here that the parthenotes/zygotes derived from enucleolated oocytes exhibited abnormal heterochromatin formation around parental pericentromeric DNAs, which led to a significant mitotic delay and frequent chromosome mis-segregation upon the first mitotic division. A proteomic analysis identified nucleoplasmin 2 (NPM2) as a dominant component of the oocyte nucleolus. Consistently, Npm2-deficient oocytes, which lack a normal nucleolar structure, showed chromosome segregation defects similar to those in enucleolated oocytes, suggesting that nucleolar loss, rather than micromanipulation-related damage to the genome, leads to a disorganization of higher-order chromatin structure in pronuclei and frequent chromosome mis-segregation during the first mitosis. Strikingly, expression of NPM2 alone sufficed to reconstitute the nucleolar structure in enucleolated embryos, and rescued their first mitotic division and full-term development. The nucleolus rescue through NPM2 required the pentamer formation and both the N-and C-terminal domains. Our findings demonstrate that the NPM2-based oocyte nucleolus is an essential platform for parental chromatin organization in early embryonic development.

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Section: Resultsmentioning

confidence: 99%

Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM2

Ogushi

Yamagata²,

Obuse

et al. 2017

Journal of Cell Science

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“…lt seems repetitive DNA sequences adjacent to the centrornere. As discussed by Broccoli et al [9], kinetochore binding to these chromosomes may be disturbed. due to modifications (mutations or changes in methylation pattern) ofthe centromeric DNA.…”

Section: Discussionmentioning

confidence: 94%

Cytogenetic changes in primary, immortalized and malignant mammalian cells

et al. 1993

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SUMMARY 283Some chromosomes in transformed rat cells and somatic cell hybrids fail to display the presence of kinetochore proteins as detected by antikinetochore antibodies. Suchchromosomes (K-Chromosomes) may constitute a novel mechanism for the genesis of aneuploidy. Wehave analyzed primary~ immortalized and malignant marnmalian cells for the presence of kinetochore proteins and micronuclei. Our resuJts suggest a correlation of the K-chromosome and micronucleus frequency with the variability in chromosome number. Upon in situ hybridization with the minor satellite and alpha satellite sequences some Kchromosomes showed a signal. This indicates that the observed lack of kinetocbores is not necessarily due to a lack of centromeric DNA. We conclude that dislocated K-chromosomes may become incorporated into micronuclei which are prone to loss. Such events would be associated with the generation of aneuploidy.

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“…In house mouse, Mus musculus, the centromere consists of two highly conserved, tandemly repeated sequences known as minor and major satellite DNA. Minor satellite DNA comprises an AT-rich, 120-bp monomer that occupies 300-600 kb of the terminal region of all mouse telocentric (Kipling et al 1991;Kalitsis et al 2006) (single-armed) chromosomes and is the site of kinetochore formation and spindle microtubule attachment (Wong and Rattner 1988;Broccoli et al 1990). Major satellite DNA is a more abundant, 234-bp tandem repeat (Horz and Altenburger 1981) that resides adjacent to a minor satellite and has a role in heterochromatin formation and sister chromatid cohesion (Guenatri et al 2004).…”

mentioning

confidence: 99%

“…Major satellite DNA is a more abundant, 234-bp tandem repeat (Horz and Altenburger 1981) that resides adjacent to a minor satellite and has a role in heterochromatin formation and sister chromatid cohesion (Guenatri et al 2004). Neither of these satellite sequences has been identified at the centromere of the morphologically distinct acrocentric Y chromosome (Broccoli et al 1990), which has a very small short arm that distinguishes it from the telocentric autosomes and X chromosome. The high degree of minor and major satellite sequence conservation that exists across the telocentric domain of all mouse telocentric chromosomes argues strongly for frequent recombinational exchanges between nonhomologous chromosomes driving sequence homogenization at mouse centromeres (Vissel and Choo 1989;Kalitsis et al 2006).…”

mentioning

confidence: 99%

Rapid evolution of mouse Y centromere repeat DNA belies recent sequence stability

Pertile¹,

Graham²,

Choo³

et al. 2009

Genome Res.

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The Y centromere sequence of house mouse, Mus musculus, remains unknown despite our otherwise significant knowledge of the genome sequence of this important mammalian model organism. Here, we report the complete molecular characterization of the C57BL/6J chromosome Y centromere, which comprises a highly diverged minor satellite-like sequence (designated Ymin) with higher-order repeat (HOR) sequence organization previously undescribed at mouse centromeres. The Ymin array is ;90 kb in length and resides within a single BAC clone that provides sequence information spanning an endogenous animal centromere for the first time. By exploiting direct patrilineal inheritance of the Y chromosome, we demonstrate stability of the Y centromere DNA structure spanning at least 175 inbred generations to beyond the time of domestication of the East Asian M.m. molossinus ''fancy'' mouse through which the Y chromosome was first introduced into the classical inbred laboratory mouse strains. Despite this stability, at least three unequal genetic exchange events have altered Ymin HOR unit length and sequence structure since divergence of the ancestral Mus musculus subspecies around 900,000 yr ago, with major turnover of the HOR arrays driving rapid divergence of sequence and higher-order structure at the mouse Y centromere. A comparative sequence analysis between the human and chimpanzee centromeres indicates a similar rapid divergence of the primate Y centromere. Our data point to a unique DNA sequence and organizational architecture for the mouse Y centromere that has evolved independently of all other mouse centromeres.

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Relationship of mouse minor satellite DNA to centromere activity

Cited by 53 publications

References 14 publications

Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM2

Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM2

Cytogenetic changes in primary, immortalized and malignant mammalian cells

Rapid evolution of mouse Y centromere repeat DNA belies recent sequence stability

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