Relationship of Nonreturn Rates of Dairy Bulls to Binding Affinity of Heparin to Sperm

Marks, Jeffrey; Ax, R. L.

doi:10.3168/jds.s0022-0302(85)81070-5

Cited by 36 publications

(18 citation statements)

References 16 publications

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“…Bulls with increased fertility produced sperm with greater affinity to bind heparin-like complex sugars that were commonly found in the reproductive tract of females (Marks and Ax 1985). It has been suggested that seminal proteins mediate the binding of sperm cells to oviductal epithelium and preserve membrane integrity by exerting inhibiting effects on the mitochondrial activity and metabolism to conserve energy needed until fertilization as well as to minimize the production of ROS and LPO of sperm membrane.…”

Section: Ai and Conception Ratementioning

confidence: 99%

Evaluation of bull semen for fertility-associated protein,in vitrocharacters and fertility

Karunakaran

Devanathan

2016

Journal of Applied Animal Research

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Proteins present in the seminal plasma and sperm influence sperm function and fertilization. The present study was carried out to screen breeding bull semen samples for the presence of fertilityassociated 28-30 kDa heparin-binding protein (HPB) and its effect on in vitro sperm characters and fertility. Semen samples were collected from 22 breeding bulls and the sperm proteins were extracted by Triton X detergent extraction method. HBPs were eluted, and the molecular weight of the proteins was assessed by discontinuous sodium dodecyl sulphate polyacrylamide gel elecrophoresis. Based on the presence/absence 28 kDa HBPs, bulls were categorized into group I and group II. Frozen semen samples were evaluated for in vitro sperm characters at immediate post thaw, 60, 120 and 180 min post-thaw incubation. To assess the field fertility of the bulls, 50 frozen semen straws/bull were used for insemination. Results indicated that only 50% of the bulls screened had 28-30 kDa HBPs in their sperm. Bulls positive for fertility-associated protein had better in vitro sperm characters, better protection against oxidative stress, readily underwent capacitation induction by heparin and had 13% higher conception than the bulls lacking the protein. So, it can be concluded that the bulls positive for of 28-30 kDa HBPs in sperm had higher chance of fertility and screening for its presence can be included in the regular breeding soundness examination for selection of bulls. ARTICLE HISTORY

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Section: Ai and Conception Ratementioning

confidence: 99%

Evaluation of bull semen for fertility-associated protein,in vitrocharacters and fertility

Karunakaran

Devanathan

2016

Journal of Applied Animal Research

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“…The ability of spermatozoa to bind heparin is also related to fertility. Five bulls with above average fertility produced sperm with higher affinity for heparin than sperm from five bulls with below average fertility (Marks and Ax, 1985). In nine bulls whose relative fertility was determined more accurately by artificial insemination of semen from pairs of the bulls into each cow and blood-typing the offspring to determine the sire, heparin affinity, and the number of binding sites to sperm were also related to fertility (Miller and Ax, 1988).…”

Section: Relationship Between Fertility and The Ability Ofmentioning

confidence: 99%

Carbohydrates and fertilization in animals

Miller

1990

Molecular Reproduction Devel

120

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A frequently used mechanism for sperrn-egg recognition in many species involves complementary protein-carbohydrate interaction. The usual paradigm includes complex glycoconjugates in reproductive tract fluids or on the eggs which are recognized by carbohydratebinding proteins on the sperm surface. Various glycoconjugates are utilized in the steps of sperm capacitation, sperm binding to the egg extracellular matrix and vitelline membrane and induction of the acrosome reaction. Several types of complex glycoconjugates are involved in these processes, including proteoglycans, lactosaminoglycans, sulfated fucose-containing glycoconiugates, and glycoproteins. There appear to be some structural similarities between active glycoconjugates; they are large in molecular weight and complex, and they are often sulfated, fucosylated, and attached to a protein through serine or threonine residues. In some species, the protein core of the glycoconjugates also participates in the interaction by limiting the binding of carbohydrates to sperm only of the relevant species, likely by providing the proper steric arrangement for the interaction. In other cases the protein core seems to serve more as a crosslinker of the carbohydrate moieties. This review discusses the types of glycoconjugates implicated in fertilization and the complementary lectinlike proteins found on sperm.Key Words: Sperm, Eggs, Glycosaminoglycans, Glycoconjugates, Glycoproteins, Zona pellucida, Capacitation, Acrosorne reaction, Fertilization cess differ between species, there is remarkable conservation in the ability of sperm to use glycoconjugates in reproductive tract secretions or in the egg extracellular matrix and vitelline membrane for fertilization-related events. This review summarizes research from various animals in which glycoconjugates are important in sperm-egg interaction. It contrasts differences between species at the point of the fertilization process where carbohydrates interact with sperm. Furthermore, a structural comparison between each of the carbohydrates is presented.An understanding of fertilization-related events is necessary prior to a discussion of the potential molecules involved in sperm-egg interaction. There are differences between species in the events occurring both before and during sperm-egg interaction. These events are best understood in sea urchins and mice, although there is recent information from other species. A diagram of sperm-egg interaction in sea urchins is shown in Figure

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“…Various functional tests have been applied to sperm including assessment of acrosome reaction induction (Lenz et al, 1988;Parkinson, 1992, 1995;Janskauskas et al, 2000), heparin binding to sperm (Marks and Ax, 1985), sperm-zona binding assays (Fazeli et al, 1993;Zhang et al, 1998), and sperm penetration assays (Ohgoda et al, 1988;Henault and Killian, 1995). Advances in bovine IVF and embryo culture techniques have allowed the relationship between in vivo bull fertility and IVF outcomes to be examined (Hillery et al, 1990;Marquant-Le Guienne et al, 1990;Shamsuddin and Larsson, 1993;Lonergan, 1994;Schneider et al, 1999;Larsson and RodriguezMartinez, 2000).…”

Section: Introductionmentioning

confidence: 99%

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Ward

Rizos

Corridan

et al. 2001

Molecular Reproduction Devel

128

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The objectives of this study were: (1) to evaluate the effect of sire on the time from insemination to first cleavage following insemination in vitro and the relationship of this parameter to field fertility and (2) to establish the relationship between the kinetics of cleavage in vitro and oocyte developmental competence for bulls of known field fertility. Frozen semen from six bulls with 150-day non-return rates ranging from 57±78% was used. In experiment 1, after insemination with semen from one of the six bulls, presumptive zygotes were transferred to IVC in droplets of synthetic oviduct fluid. Droplets were examined at 24, 27, 30, 33, 36, 42, and 48 hr after insemination and the number of cleaved oocytes was recorded. Blastocyst yield was recorded on Days 6-, 7-, and 8-post insemination. In experiment 2, culture droplets were examined at 30, 36, and 48 hr after insemination. At each time point, the number of cleaved embryos was recorded and these embryos were transferred into new droplets and were cultured separately for the duration of the experiment. The proportion of embryos developing to the blastocyst stage was recorded for each of the groups for each bull. The best predictor of field fertility was a model containing 33-hpi-cleavage percentage only (r 0.689, P`0.0001). There was also a significant correlation between blastocyst yield and non-return rate, with Day 7 blastocyst yield having the highest correlation (r 0.356), although this was relatively low in comparison. In experiment 2, irrespective of sire, a significantly higher proportion of those early-cleaving oocytes (before 30 hpi) developed to blastocysts than those cleaving later. In most cases, a higher proportion of blastocysts derived from early-cleaving oocytes hatched from the zona pellucida suggesting that such blastocysts are of superior quality to those derived from late-cleaving oocytes. In conclusion these data confirm our earlier observations that earliest cleaving zygotes are more competent in terms of development to the blastocyst stage than those that cleave later. This phenomenon is independent of the sire used. However, we have demonstrated that the kinetics of early embryonic development as measured by the timing of the first cleavage division post insemination vary between different bulls and that these differences can be used to discriminate between bulls of high and low bull field fertility. Mol. Reprod. Dev. 60: 47±55,

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Relationship of Nonreturn Rates of Dairy Bulls to Binding Affinity of Heparin to Sperm

Cited by 36 publications

References 16 publications

Evaluation of bull semen for fertility-associated protein,in vitrocharacters and fertility

Evaluation of bull semen for fertility-associated protein,in vitrocharacters and fertility

Carbohydrates and fertilization in animals

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

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