Relationship of physicochemical factors with fish biomass and production in Shadegan Wetland, Iran

Abstract

Bouea macrophylla Griff. is well-known as one of native typical fruits in Southeast Asia which needs to be preserved and continuously cultivated because of economical and ecological significances. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. This technique has proven to be robust and highly variable and is attained through a significantly less technically demanding process. In this research, SRAP method was preliminary applied to assess genetic characterization of B. macrophylla. Genomic DNA was extracted from fresh leaf samples. The result clearly showed that at 100 ng template DNA and MgCl 2 5 mM concentration are suitable for further PCR analysis. Thirty SRAP primer combinations were initially screened for analysis and 26 primer combinations were chosen for further analysis. A total of 222 DNA fragments, varying from 90-2500 bp, were amplified. The produced band number for each optimal primer set ranged from 3 to 12 with a percentage of polymorphic bands spanning from 33.33 to 80.00%. Therefore, SRAP analysis is suitable for further analysis method on genetic study of Bouea species and related genera.

Virucidal activity of silver nanoparticles against Banana bunchy top virus (BBTV) in banana plants

“…Whereas, SRAP analysis gave the highest number of unique markers, compared with RAPD due to SRAP targets the coding region ORFs (open reading frames) (Liao et al 2012). Exons are usually rich with GC contents and the 'CCGG' sequence in the core of the forward SRAP primers is designed to target such coding sequences (Shao et al 2010;Kaewpongumpa et al 2016). Besides, the RAPD primers amplify both coding and noncoding DNA sequence of the banana genome, but when it amplifies in one region it does not amplify in another, so decrease the possibility of amplifying the most polymorphic sequences (McGregor et al 2000).…”

Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia

“…The extent polymorphism is important to identify accurate associations among accessions (Bhatt et al 2017). The level of polymorphism was comparatively higher than previous studies on many crops such as in Pinus, the level of polymorphism is 55% (Feng et al 2009) and 57.27% in Bouea macrophylla (Kaewpongumpai et al 2016). 2.…”

“…The knowledge about the genetic diversity of crop is essential in genetic improvement programs (Wu et al 2014), can assist the development of efficient germplasmmanagement and utilization strategies (Kaewpongumpai et al 2016) and the keys of success of breeding programs (Kumar et al 2017). Several parameters are used for analyzing the genetic diversity, that is the mean of the observed number of alleles (Na), the mean of an effective number of alleles (Ne), the percentage of polymorphic loci (PPL), Nei's gene diversity (H) and Shannon's Information index (I).…”

“…SRAP markers is also a useful DNA fingerprinting tool for evaluation of genetic diversity of species, cultivars and breeding line such as in cumin (Amar et al 2011;Bhatt et al 2017), Vigna radiata (Aneja et al 2013), tuber crop Dioscorea spp. (Wu et al 2014), and Bouea macrophylla accessions (Kaewpongumpai et al 2016).…”

Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand

“…Following this, the DNA was extracted using CTAB DNA extraction protocol without RNase. The process was repeated until the DNA pellet was free of color (two to three times) and the final pellet was dissolved in sterile deionized water (Vanijajiva 2014;Kaewpongumpai et al 2016). DNA quality was using Nanodrop Spectrophotometer (Thermo scientific Nanodrop 1000, USA) at the absorbance ratio of 260 and 280 nm providing a value of 1.7-1.8 which determines pure DNA preparation.…”