Relationship of RNA/DNA ratio and temperature to growth in larvae of Atlantic cod Gadus morhua

Caldarone, Elaine M.; StOnge-Burns, JM; Buckley, LJ

doi:10.3354/meps262229

Cited by 66 publications

(61 citation statements)

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“…After standardizing the RD values, data from calibration experiments with Atlantic cod and haddock (Caldarone et al 2003, Caldarone 2005 were reanalyzed and models containing all linear combinations of sRD, T, and sRD × T were constructed (Table 3) Table 3) in relation to fish species using the growth model data set (Table 2). Full species' names in Table 2 0 5 10 15 20 25 30 Temperature ( (Table 2) AIC, the best combined cod and haddock model included the main effect of sRD, the interaction term, and an intercept (Eq.…”

Section: Resultsmentioning

confidence: 99%

“…In most instances, the addition of terms for larval age, size, or feeding level to these models did not significantly decrease the residual sum of squares, indicating that the relationship among RD, T and G was independent of these variables (e.g. Buckley 1982, Caldarone et al 2003, Caldarone 2005; i.e. while RD and G are known to follow ontogenetic trends (e.g.…”

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confidence: 99%

“…While the amount of ribosomal RNA sets the capacity for protein synthesis, DNA remains relatively constant in a cell. Thus over the past 3 decades, RD has been widely used as an index of nutritional condition (Clemmesen 1994, Grønkjaer et al 1997, Buckley et al 1999, St. John et al 2001) and, to a lesser extent, as a means to estimate weight-specific growth rate (G) in fish larvae (Buckley 1984, Caldarone et al 2003, Caldarone 2005, Malzahn et al 2007. Since the actual rate of translation (protein synthesis per unit RNA) varies with temperature (Millward et al 1973, Preedy et al 1988, Farewell & Neidhardt 1998, estimating G from RD in ectotherms requires an adjustment for the activity of RNA due to temperature.…”

Section: Introductionmentioning

confidence: 99%

“…The 6 species in this data set were considered to be 'well-fed' by the authors of the studies. Although the sRD data showed considerable variability, no trend in the values with temperature ( After standardizing the RD values, data from calibration experiments with Atlantic cod and haddock (Caldarone et al 2003, Caldarone 2005 were reanalyzed and models containing all linear combinations of sRD, T, and sRD × T were constructed (Table 3) Relationship between observed instantaneous growth rates (G) of larvae in the growth model data set and growth rates estimated with the best-fit meta-analysis growth model (Eq. 1 in Table 3).…”

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confidence: 99%

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Multi-species larval fish growth model based on temperature and fluorometrically derived RNA/DNA ratios: results from a meta-analysis

Buckley¹,

Caldarone²,

Clemmesen³

2008

Mar. Ecol. Prog. Ser.

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Weight-specific growth rate (G) and growth performance (the fraction of maximum growth realized, G pf ) are key demographic characteristics. The ratio of RNA/DNA (RD) can provide information on both G and G pf . Estimating G from RD in larval fish requires an adjustment for the activity of RNA at different temperatures. Based on a meta-analysis of published data, we present a general model for the relationship between G in marine fish larvae and fluorometrically derived RD and temperature (T), and suggest that this model can be used to estimate G in marine fish larvae. Several options for estimating G pf are also considered, including the use of a reference growth rate (G ref ). RDs of well-fed larvae appeared to be independent of water temperatures between 4 and 28°C, suggesting that any increase in growth rate with temperature was accomplished by increased activity rather than increased concentrations of RNA. However, for the best-fit meta-analysis RD-T-G model, the relationship between RD and G pf was temperature dependent for fish less than fully fed.KEY WORDS: RNA/DNA ratio · Growth · Larvae · Temperature effects · Nucleic acids · Fluorometric microplate assay · Multi-species meta-analysis Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 371: [221][222][223][224][225][226][227][228][229][230][231][232] 2008 among RD, temperature (T) and G (RD-T-G relationship). These calibration experiments require specialized rearing facilities, are costly, and labor intensive. Consequently, only a few calibrations are available for a very limited number of species (Table 1), thus hampering the widespread use of RD for estimating G in fish larvae.A variety of analytical methods have been used to estimate RNA and DNA concentrations in fish larvae. Because RD values are sensitive to the choice of procedures used, any meta-analysis of RD values from different laboratories has been problematic. This issue was addressed in a recent international intercalibration effort ) whereby fluorometrically derived RD estimates were standardized based on the slopes of the RNA and DNA standard curves (Berdalet et al. 2005). This standardization method opens up both the possibility of comparing fluorometrically derived RD among laboratories, and using RD-T-G relationships developed in other laboratories. Buckley (1984), using an UV method for analysis of RD, presented a RD-T-G calibration model based on data from larvae of 8 species of temperate marine fishes (Table 1), and suggested that there was a single relationship among RD-T-G that was independent of species. Although this general model has been used for several species, the conclusion that there is a single RD-T-G relationship in larvae of temperate marine fishes has not been further tested. Moreover, no general model based on the newer fluorometric methods has been published. To examine these and other issues, we propose the following hypotheses: (1) A single RD-T-G relationship can be used for all species of marine fish lar...

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Multi-species larval fish growth model based on temperature and fluorometrically derived RNA/DNA ratios: results from a meta-analysis

Buckley¹,

Caldarone²,

Clemmesen³

2008

Mar. Ecol. Prog. Ser.

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“…Nucleic acid analysis is an acknowledged practical tool for studying recent overall nutritional condition and growth of larvae and young fish, as well as their responses to environmental variability (e.g. Clemmesen et al, 1997;Chícharo, 1997;Buckley et al, 1999;Caldarone et al, 2003). Specifically, RNA/DNA ratio reflects variations in protein synthesis rates: the quantity of DNA in an animal somatic cell is believed to be normally stable, but the quantity of RNA primarily associated with ribosomes is closely related to the rate of protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Influence of starvation on the critical swimming behaviour of the Senegalese sole (<i>Solea senegalensis</i>) and its relationship with RNA/DNA ratios during ontogeny

Faria¹,

Muha²,

Morote³

et al. 2010

Sci. Mar.

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SUMMARY: Food availability can affect larval survival directly through starvation and indirectly through the effects on larval growth rate, swimming performance and vulnerability to predators. In the present study we evaluate the effects of starvation on growth, nutritional condition and swimming behaviour of the Senegalese sole (Solea senegalensis) throughout ontogeny (8 to 14 days after hatching). Biochemical analysis (RNA/DNA ratios) and behavioural experiments (critical swimming speed, U crit ) were conducted on larvae reared under 3 feeding treatments: fed ad libitum, deprived of food for 48 hours and deprived of food for 96 hours. Growth was significantly affected by feeding treatment, while only slight decreases in RNA/ DNA ratio and swimming performance were registered. Late stage larvae of the three feeding treatments had slower critical speeds than the pre-flexion and flexion stages, which is probably related to the benthic lifestyle acquired by the species at the end of the larval period. These physiological and behavioural changes are in accordance with previous results, which show that flatfish larvae are more resistant to starvation than pelagic species and that they become less active later in development.Keywords: Senegalese sole, Solea senegalensis, feeding treatment, starvation, RNA/DNA ratio, critical swimming speed, U crit , ontogeny. RESUMEN: Influencia del ayuno sobre el comportamiento natatorio crítico del lenguado senegalés Solea SenegalenSiS y su relación con el cociente RNA/DNA durante la ontogenia. -La disponibilidad de comida puede afectar la supervivencia larvaria directamente a través del ayuno e indirectamente a través de los efectos sobre la tasa de crecimiento larvario, actividad natatoria y vulnerabilidad a los predadores. En el presente estudio evaluamos los efectos del ayuno sobre el crecimiento, condición nutricional y comportamiento natatorio del lenguado senegalés, Solea senegalensis, durante la ontogenia (8 a 14 días post-eclosión). Se llevaron a cabo análisis bioquímicos (RNA/DNA) y experimentos de comportamiento (velocidad crítica de natación, U crit ) sobre larvas mantenidas bajo 3 condiciones de alimentación: alimentadas (tratamiento ad libitum), ayuno a corto plazo (hasta 48 h sin alimentación) y ayuno a largo plazo (hasta 96 h sin alimentación). El crecimiento varió significativamente según el tratamiento alimentario, mientras que el cociente RNA/DNA y la capacidad natatoria mostraron únicamente una ligera disminución. En los tres tratamientos alimentarios los estadios larvarios más avanzados mostraron velocidades críticas más bajas cuando se compararon con las de los estados de preflexión y flexión, lo que está probablemente relacionado con el estilo de vida bentónica adquirido por la especie al final del periodo larvario. Estos cambios fisiológicos y de comportamiento concuerdan con resultados previos que muestran que las larvas de peces planos son más resistentes al ayuno que las de especies pelágicas y se vuelven menos activas con el desarrollo.Palabras clave: le...

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Recruitment Variability

Houde¹

2016

Fish Reproductive Biology

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Relationship of RNA/DNA ratio and temperature to growth in larvae of Atlantic cod Gadus morhua

Cited by 66 publications

References 39 publications

Multi-species larval fish growth model based on temperature and fluorometrically derived RNA/DNA ratios: results from a meta-analysis

Multi-species larval fish growth model based on temperature and fluorometrically derived RNA/DNA ratios: results from a meta-analysis

Influence of starvation on the critical swimming behaviour of the Senegalese sole (<i>Solea senegalensis</i>) and its relationship with RNA/DNA ratios during ontogeny

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