Relationship of the cycle threshold values of SARS-CoV-2 polymerase chain reaction and total severity score of computerized tomography in patients with COVID 19

Yağcı, Ayşegül; Sarınoğlu, Rabia Can; Bilgin, Hüseyin; Yanılmaz, Özgür; Sayın, Elvan; Deniz, Guneser; Güncü, Mehmet Mucahit; Doyuk, Zahide; Can, Barış; Kuzan, Beyza Nur; Aslan, Bülent; Korten, Volkan; Çimşit, Çağatay

doi:10.1016/j.ijid.2020.09.1449

Cited by 54 publications

(42 citation statements)

References 16 publications

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“…For other viral illnesses, the initial viral load has been associated with disease severity [ 18 , 19 ], however, a consensus has not been reached regarding COVID-19. To the best of our knowledge, 11 studies have shown that nasopharyngeal SARS-CoV-2 Ct are not associated with, or predict, COVID-19 severity [ 13 , 14 , 15 , 20 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. However, only six of them analyzed samples with >100 cases, the majority included only hospitalized patients, and the follow-up was relatively short.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic SARS-CoV-2 Cycle Threshold Value Predicts Disease Severity, Survival, and Six-Month Sequelae in COVID-19 Symptomatic Patients

Trunfio

Venuti

Alladio

et al. 2021

Viruses

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To date, there is no severe acute respiratory syndrome coronavirus 2- (SARS-CoV-2)-specific prognostic biomarker available. We assessed whether SARS-CoV-2 cycle threshold (Ct) value at diagnosis could predict novel CoronaVirus Disease 2019 (COVID-19) severity, clinical manifestations, and six-month sequelae. Hospitalized and outpatient cases were randomly sampled from the diagnoses of March 2020 and data collected at 6 months by interview and from the regional database for COVID-19 emergency. Patients were stratified according to their RNA-dependent-RNA-polymerase Ct in the nasopharyngeal swab at diagnosis as follows: Group A ≤20.0, 20.0< group B ≤28.0, and Group C >28.0. Disease severity was classified according to a composite scale evaluating hospital admission, worst oxygen support required, and survival. Two hundred patients were included, 27.5% in Groups A and B both, 45.0% in Group C; 90% of patients were symptomatic and 63.7% were hospitalized. The median time from COVID-19 onset to swab collection was five days. Lethality, disease severity, type, and number of signs and symptoms, as well as six-month sequelae distributed inversely among the groups with respect to SARS-CoV-2 Ct. After controlling for confounding, SARS-CoV-2 Ct at diagnosis was still associated with COVID-19-related death (p = 0.023), disease severity (p = 0.023), number of signs and symptoms (p < 0.01), and presence of six-month sequelae (p < 0.01). Early quantification of SARS-CoV-2 may be a useful predictive marker to inform differential strategies of clinical management and resource allocation.

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Section: Discussionmentioning

confidence: 99%

Diagnostic SARS-CoV-2 Cycle Threshold Value Predicts Disease Severity, Survival, and Six-Month Sequelae in COVID-19 Symptomatic Patients

Trunfio

Venuti

Alladio

et al. 2021

Viruses

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“…Taking into account that Ct values may indirectly reflect the viral load in a given patient, our results suggest that older SARS-CoV-2-infected individuals have higher viremia at diagnosis ( Tom and Mina, 2020 ), which may at least partly explain more severe course of the disease and worse prognosis ( Karahasan Yagci et al, 2020 ). A relationship between lower Ct values and higher clinical severity has already been found years ago in patients with various upper respiratory tract infections, caused by adenovirus, human metapneumovirus, and parainfluenza virus ( Fuller et al, 2013 ).…”

Section: Resultsmentioning

confidence: 96%

Aging correlates with lower threshold cycle values for RdRP/RdRP+S genes during molecular detection of SARS-CoV-2

Mikuła-Pietrasik

Książek

2021

Experimental Gerontology

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RT-qPCR is the most reliable molecular method for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we analyzed results of RT-qPCR obtained for 3044 patients diagnosed as SARS-CoV-2-positive using four different molecular tests utilizing five RNA sequences. The analysis showed that patients' age inversely correlates with threshold cycle ( Ct ) values for RdRP gene (LightMix® Modular Wuhan CoV RdRP-gene by Roche Diagnostics) and RdRP+S genes (MutaPLEX® Coronavirus RT-PCR kit by Immundiagnostic). At the same time, there was no correlation between age and Ct values for E , N , and ORF1ab genes. When patients were grouped by age, mean Ct values for RdRP gene in older patients were significantly lower compared with younger individuals. Collectively, our report indicates that older SARS-CoV-2-infected individuals exhibit higher viremia at diagnosis than younger patients, which may reflect impaired functioning of their immune response and predispose to more severe disease and worse prognosis.

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“…Here, the key is to have access to patient oro- or nasopharyngeal samples in viral transport medium (VTM) that display low Ct values in the quantitative real time RT-PCR assays. The association of the viral load estimated by qRT-PCR with COVID severity is controversial [21, 22], and qRT-PCR-generated Ct values of viral genes are not true indicators of the viral load owing to a large number of variables in sample collection and processing and hence could be deceptive at times. Therefore it is important to try several VTM samples that display Ct values below 30.…”

Section: Resultsmentioning

confidence: 99%

Inactivation of SARS-CoV-2 by β-propiolactone Causes Aggregation of Viral Particles and Loss of Antigenic Potential

Gupta

Parthasarathy

Sah

et al. 2021

Preprint

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Inactivated viral preparations are important resources in vaccine and antisera industry. Of the many vaccines that are being developed against COVID-19, inactivated whole-virus vaccines are also considered effective. β-propiolactone (BPL) is a widely used chemical inactivator of several viruses. Here, we analyze various concentrations of BPL to effectively inactivate SARS-CoV-2 and their effects on the biochemical properties of the virion particles. BPL at 1:2000 (v/v) concentrations effectively inactivated SARS-CoV-2. However, higher BPL concentrations resulted in the loss of both protein content as well as the antigenic integrity of the structural proteins. Higher concentrations also caused substantial aggregation of the virion particles possibly causing undesirable outcomes including a potential immune escape by infectious virions, and a loss in antigenic potential. We also identify that the viral RNA content in the culture supernatants can be a direct indicator of their antigenic content. Our findings may have important implications in the vaccine and antisera industry during COVID-19 pandemic.

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Relationship of the cycle threshold values of SARS-CoV-2 polymerase chain reaction and total severity score of computerized tomography in patients with COVID 19

Cited by 54 publications

References 16 publications

Diagnostic SARS-CoV-2 Cycle Threshold Value Predicts Disease Severity, Survival, and Six-Month Sequelae in COVID-19 Symptomatic Patients

Diagnostic SARS-CoV-2 Cycle Threshold Value Predicts Disease Severity, Survival, and Six-Month Sequelae in COVID-19 Symptomatic Patients

Aging correlates with lower threshold cycle values for RdRP/RdRP+S genes during molecular detection of SARS-CoV-2

Inactivation of SARS-CoV-2 by β-propiolactone Causes Aggregation of Viral Particles and Loss of Antigenic Potential

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