Relationships among frozen-thawed sperm characteristics assessed via the routine semen analysis, sperm functional tests and fertility of bulls in an artificial insemination program

Correa, Juan R.; Pace, M. M.; Zavos, Panayiotis M.

doi:10.1016/s0093-691x(97)00296-3

Cited by 145 publications

(111 citation statements)

References 30 publications

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“…An in vitro assay for determining bull fertility, which could replace ®eld fertility trials, would be of great bene®t to AI programmes. Many methods have been used for the assessment of bull sperm viability following cryopreservation including examination of motility (Kjaestad et al, 1993;Correa et al, 1997), ATP measurements (Janskauskas and Rodriguez-Martinez, 1995), membrane integrity (Harrison and Vickers, 1990;Janskauskas and Rodriguez-Martinez, 1995) and measurement of osmotic resistance (Correa and Zavos, 1994;Revell and Mrode, 1994;Rota et al, 2000). The results of such assays do not always correlate with the fertility of a semen sample.…”

Section: Introductionmentioning

confidence: 99%

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Ward

Rizos

Corridan

et al. 2001

Molecular Reproduction Devel

128

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The objectives of this study were: (1) to evaluate the effect of sire on the time from insemination to first cleavage following insemination in vitro and the relationship of this parameter to field fertility and (2) to establish the relationship between the kinetics of cleavage in vitro and oocyte developmental competence for bulls of known field fertility. Frozen semen from six bulls with 150-day non-return rates ranging from 57±78% was used. In experiment 1, after insemination with semen from one of the six bulls, presumptive zygotes were transferred to IVC in droplets of synthetic oviduct fluid. Droplets were examined at 24, 27, 30, 33, 36, 42, and 48 hr after insemination and the number of cleaved oocytes was recorded. Blastocyst yield was recorded on Days 6-, 7-, and 8-post insemination. In experiment 2, culture droplets were examined at 30, 36, and 48 hr after insemination. At each time point, the number of cleaved embryos was recorded and these embryos were transferred into new droplets and were cultured separately for the duration of the experiment. The proportion of embryos developing to the blastocyst stage was recorded for each of the groups for each bull. The best predictor of field fertility was a model containing 33-hpi-cleavage percentage only (r 0.689, P`0.0001). There was also a significant correlation between blastocyst yield and non-return rate, with Day 7 blastocyst yield having the highest correlation (r 0.356), although this was relatively low in comparison. In experiment 2, irrespective of sire, a significantly higher proportion of those early-cleaving oocytes (before 30 hpi) developed to blastocysts than those cleaving later. In most cases, a higher proportion of blastocysts derived from early-cleaving oocytes hatched from the zona pellucida suggesting that such blastocysts are of superior quality to those derived from late-cleaving oocytes. In conclusion these data confirm our earlier observations that earliest cleaving zygotes are more competent in terms of development to the blastocyst stage than those that cleave later. This phenomenon is independent of the sire used. However, we have demonstrated that the kinetics of early embryonic development as measured by the timing of the first cleavage division post insemination vary between different bulls and that these differences can be used to discriminate between bulls of high and low bull field fertility. Mol. Reprod. Dev. 60: 47±55,

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Section: Introductionmentioning

confidence: 99%

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Ward

Rizos

Corridan

et al. 2001

Molecular Reproduction Devel

128

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“…Januskauskas et al (2003) detected significant correlations between field fertility and PMI assessed by PI. Correa et al (1997) demonstrated a positive correlation with PMI and high fertility bulls. Batches 3 and 4 presented similar high percentages of PMI after thawing (Control Group).…”

Section: Resultsmentioning

confidence: 85%

“…In this sense, several researchers have reported a marked variability in field fertility among sires and/or batches (CORREA et al, 1997;WARD et al, 2001;ANDERSSON et al, 2004;OLIVEIRA et al, 2012a). Differences in field fertility could be attributed to variations in sperm-qualitative characteristics (CORREA et al, 1997). Different ejaculates from the same bull may present different qualitative and/or quantitative sperm traits, depending on several factors as age, animal handling, nutrition, season of the year, animal health, and others.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, several studies already demonstrated varied conception rates depending on the semen batch and/ or sire utilized (CORREA et al, 1997;ZHANG et al, 1999;ANDERSSON et al 2004;SÁ FILHO et al, 2009;OLIVEIRA et al, 2013;SANTOS, 2016). According to Sellem et al (2015), predicting in vivo fertility of bull ejaculates based on in vitro semen characteristics remains challenging for the breeding industry.…”

Section: Introductionmentioning

confidence: 91%

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Assessment of different in vitro sperm challenges and in vivo fertility of bovine semen batches

Oliveira

Ribeiro

Silva

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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The aim of this work was to submit sperm cells to different laboratory challenges and to compare in vitro results with in vivo semen fertility. Four different batches from the same Brangus bull were used in a timed-AI program of 332 Brangus cows. Each batch (B) was submitted to the following procedure: semen sample was thawed at 36°C for 30 seconds (control). Sperm motility parameters, plasma membrane integrity, sperm morphology, and concentration were assessed. Then, an aliquot of thawed sample was incubated in a water bath at 45°C for 40 min (thermal challenge group; TCG) and another aliquot was centrifuged at 500 xg (Percoll gradient 45%/90%) for 15 min (centrifugation challenge group; CCG). Centrifuged semen was also submitted to another thermal challenge, being incubated (water bath) at 45°C for 40 min (centrifugation + thermal challenge group; CTCG). At the end of each challenge (CCG, TCG, and CTCG), the same laboratory tests used for control group were repeated. The following conception rates (CR) were observed for each batch: B1 = 48.9% (44/90); B2 = 44.2% (23/52); B3 = 55.5% (40/72); B4 = 43.2% (51/118); (p < 0.10). In the lab, B3 presented higher (p ≤ 0.05) progressive motility (PM) than B4 after thawing (control group) and after all sperm challenges (TCG, CCG, and CTCG). However, despite B3 and B4 having demonstrated a similar percentage of plasma membrane integrity (PMI) to the control group (B3 = 66.7 ± 1.3 and B4 = 65.2 ± 3.3), B3 demonstrated higher (P ≤ 0.05) percentage of PMI (37.2 ± 2.5) than B4 (26.7 ± 3.3) after passing through the most stressing in vitro challenge (CTCG). The semen batch presenting the highest resistance to in vitro challenges was the one that presented a trend for higher in vivo fertility, suggesting that submitting semen samples to laboratory challenges may be an interesting alternative for selecting batches with greater field fertility. Keywords: Conception rate. In vitro sperm resistance. Timed-AI. ResumoO objetivo deste estudo foi estressar células espermáticas em diferentes desafios laboratoriais e comparar os resultados in vitro com a fertilidade in vivo do sêmen. Quatro partidas de um mesmo touro Brangus foram utilizadas em um programa de IATF de 332 vacas Brangus. Cada partida foi submetida ao seguinte procedimento: a amostra de sêmen foi descongelada a 36°C por 30 segundos (grupo controle). Foram avaliados parâmetros de motilidade espermática (CASA), integridade da membrana plasmática (PMI), morfologia e concentração espermática. Em seguida, uma alíquota da amostra descongelada foi incubada em banho-maria a 45°C durante 40 minutos (grupo de desafio térmico, TCG) e outra alíquota foi centrifugada a 500 xg (gradiente de Percoll 45%/90%) durante 15 min (grupo desafio de centrifugação, CCG). Uma aliquota do sêmen centrifugado foi ainda submetida ao desafio térmico, sendo incubado a 45°C durante 40 min (grupo de desafio térmico + centrifugação, CTCG). No final de cada desafio (CCG, TCG e CTCG), os mesmos testes laboratoriais utilizados para o grupo de controle f...

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“…O teste de resistência osmótica foi considerado como um indicador de fertilidade em diversas espécies: humanos (JEYENDRAN et al, 1984;VAN DER VEN et al, 1986;CHAN, et al, 1991;HOSSAIN et al, 1998), eqüinos (NIE et al, 2001;MELO;HENRY, 1999;NEILD et al, 1999), caninos (KUMI-DIAKA, 1993), suínos (PÉREZ-LLANO et al, 2001) e touros (CORREA et al, 1997;ROTA et al, 2000;TARTAGLIONE;RITTA, 2004). Consiste em um método fácil, acessível e barato (CORREA; ZAVOS, 1994;TARTAGLIONE;RITTA, 2004).…”

Section: I T E R a T U R A | 33unclassified

Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo

Gallego¹

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A verdade é que, quando sua mente estava completamente alheia, ele tinha o pensamento mais estranho que qualquer lunático no mundo jamais tivera, pois lhe parecia razoável e necessário, tanto por uma questão de honra quanto para servir a nação, se tornar um cavaleiro errante e viajar pelo mundo em sua armadura e seu cavalo para buscar aventuras e envolver-se em tudo que ele havia lido sobre como os cavaleiros errantes se envolviam, aproveitando as oportunidades, colocando-se em perigo e corrigindo todo tipo de mal.-Miguel de Cervantes, Don Quixote. DEDICO.A Dios.A mis Papas Ramiro y Alba Lucia, su amor es la base de todas mis logros, Dios me bendijo al poder tenerlos a mi lado.A mi hermanita María del Pilar, eres la otra faz de una obra de arte, tu alegría y emotividad me llenan de felicidad. No te imaginas como te admiro.A mis abuelitas Ruth y Mary, Las abuelitas más dulces y tiernas que puedan existir, siempre las llevo en mi corazón.A mis Abuelos Libardo y Jorge (In memoriam), Sus vidas son una inspiración para mi. Es un honor llevar el apellido Mejia Gallego. Los Extraño A mi novia Dunia, Porque nuestros sueños se hagan realidad. LOS AMO !!!!! Agradecimientos.A mis tias Angela, Nohora y Liliana: Siempre han creido en mis capacidades, ello me ha llenado de confianza para llegar a donde estoy ahora, de todas he aprendido cosas importantes en la vida.Las llevo y las llevare en mi corazón siempre.A mis tios

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Relationships among frozen-thawed sperm characteristics assessed via the routine semen analysis, sperm functional tests and fertility of bulls in an artificial insemination program

Cited by 145 publications

References 30 publications

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Assessment of different in vitro sperm challenges and in vivo fertility of bovine semen batches

Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo

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