Relationships Among Metallothionein, Cadmium Accumulation, and Cadmium Tolerance in Three Species of Fish

Wu, Su Mei; Jong, Koa-Jen; Lee, Y. J.

doi:10.1007/s00128-006-0961-7

Cited by 29 publications

(10 citation statements)

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“…Cadmium toxicology has been studied in various aquaculture organisms (Wu et al 2006;Seebaugh et al 2012;Ma et al 2013). and previous studies have demonstrated that Hsps are involved in the defense against cadmium toxicity (Jing et al 2013;Jung and Lee 2013;Mazzei et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Shi

Zhao

et al. 2016

Cell Stress and Chaperones

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Heat shock proteins (Hsps) are a class of highly conserved proteins produced in virtually all living organisms from bacteria to humans. Hsp60 and Hsp10, the most important mitochondrial chaperones, participate in environmental stress responses. In this study, the full-length complementary DNAs (cDNAs) of Hsp60 (PmHsp60) and Hsp10 (PmHsp10) were cloned from Penaeus monodon. Sequence analysis showed that PmHsp60 and PmHsp10 encoded polypeptides of 578 and 102 amino acids, respectively. The expression profiles of PmHsp60 and PmHsp10 were detected in the gills and hepatopancreas of the shrimps under pH challenge, osmotic stress, and heavy metal exposure, and results suggested that PmHsp60 and PmHsp10 were involved in the responses to these stimuli. ATPase and chaperone activity assay indicated that PmHsp60 could slow down protein denaturation and that Hsp60/Hsp10 may be combined to produce a chaperone complex with effective chaperone and ATPase activities. Overall, this study provides useful information to help further understand the functional mechanisms of the environmental stress responses of Hsp60 and Hsp10 in shrimp.

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Section: Discussionmentioning

confidence: 99%

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Shi

Zhao

et al. 2016

Cell Stress and Chaperones

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“…Vertical bars denote 0.95 confidence intervals. native organ, due to the activities of metallothioneins, proteins with the ability to bind to specific metals, such as Cu, Cd and Zn, thereby reducing their toxicity and allowing for the accumulation of high metal concentrations [20,22,23]. In our study, the highest concentrations of most of the analyzed metals were detected in the gills (Zn, Sr, Pb, Ni, Mn, Li, Cr, Ba, and B).…”

Section: Discussionmentioning

confidence: 48%

Concentrations of metals and trace elements in different tissues of nine fish species from the Medjuvrsje reservoir (West Morava River Basin, Serbia)

Đikanović

Skorić

Gačić

2016

Arch biol sci (Beogr)

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Element concentrations in selected fish species from different trophic levels were analyzed. The following fish species were analyzed: common nase (Chondrostoma nasus), roach (Rutilus rutilus), freshwater bream (Abramis brama), barbel (Barbus barbus), Prussian carp (Carassius gibelio), chub (Squalius cephalus), European perch (Perca fluviatilis), wels catfish (Silurus glanis) and northern pike (Esox lucius). Fish were collected from the Međuvršje Reservoir (West Morava River Basin, western Serbia) during 2012, and samples of liver, muscle and gills were analyzed for As,

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“…has been found to cause a timeand dose-dependent increase in Cd 2? contents in various fish species (Wu et al 2006;Firat et al 2009). Accumulation of Cd 2?…”

Section: Discussionmentioning

confidence: 98%

Anti-oxidative functions of mt2 and smtB mRNA expression in the gills and brain of zebrafish (Danio rerio) upon cadmium exposure

Shu

2016

Fish Physiol Biochem

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There were not any past studies about metallothionein isoforms (smtB and mt2) having anti-oxidative functions on zebrafish after Cd exposure. On the other hand, the anti-oxidative enzymatic factors such as superoxide dismutase (sod), glutathione peroxidase (gpx1a), and catalase (cat) are used as references to investigate whether the smtB and mt2 have anti-oxidative responses on the gills and brain of zebrafish after 1-6 h of 0 and 1.78 μM Cd exposure. The anti-oxidative system such as sod, cat, and gpx1a mRNA expressions demonstrated a cascade response upon Cd-induced oxidative stress in the present study. Interestingly, the smtB mRNA expression levels increased by 3.2- to 6.1-fold, and mt2 raised by 4.1- to 11.3-fold in gills at 1 and 3 h after exposure to Cd, respectively. On the other hand, the smtB mRNA levels increased by 10.6- to 58.6-fold, but mt2 mRNA levels increased by 2.3- to 11.1-fold in brain at 1 and 3 h after exposure to Cd, respectively. In addition, both tissues showed increased apoptosis levels at 3 h, and recovery after 6 h of Cd exposure. From the results, we suggest that both mt2 and smtB play a role in anti-oxidation responses within 6 h after exposure to Cd. In conclusion, the smtB mRNA levels have a higher response than mt2 in the brain, but both mRNA expressions appear to have a similar pattern in the gill. We suggest that smtB plays an important role to defend oxidative stress in the brain of adult zebrafish upon acute Cd exposure.

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Relationships Among Metallothionein, Cadmium Accumulation, and Cadmium Tolerance in Three Species of Fish

Cited by 29 publications

References 0 publications

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Concentrations of metals and trace elements in different tissues of nine fish species from the Medjuvrsje reservoir (West Morava River Basin, Serbia)

Anti-oxidative functions of mt2 and smtB mRNA expression in the gills and brain of zebrafish (Danio rerio) upon cadmium exposure

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